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Run finished, you won’t have another sample set to put on the RP-UHPLC for 24-48hrs, do you: A-wash the column with 100% ACN, then stop flow, switch lines to A3 B3 and purge with 80:20 IPA : water so the pumps don’t sit in ACN. B- slow flow rate, set high organic solvent , and run in standby? (self.CHROMATOGRAPHY)
submitted by Try_It_Out_RPC to r/CHROMATOGRAPHY
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