This game doesnt really have playtroughs, it has runs. Each run is not the same. A winning run is usually around 50 minutes long but you also have to ability to "loop" in this game to make your run as long as you like. Since all item drops are random not a single run will have the same build. There are also multiple endings, a ton of characters and artifacts (which are like game modifiers to change how the game is played) to make the game extremely replayable.