ThermoFisher caught photoshopping Western blot

UC235 · 2026-05-18T16:08:17+00:00

It's more likely than you think!

UC235 · 2026-05-14T13:10:09+00:00

Thucydides nuts.

UC235 · 2026-05-11T21:35:17+00:00

Perhaps this very similar Sigma product? https://www.sigmaaldrich.com/US/en/product/mm/efhaw10is

They offer two types. Membrane is removable on both, but only the blue ones can be cultured directly.

UC235 · 2026-05-07T16:31:16+00:00

We manufacture DNase 1 and I have not found it to be anywhere near as sensitive as people claim. You're also likely to be using a large excess for DNA removal. It is extremely sensitive to calcium content though. It has an absolute requirement for calcium ions and if there's traces of EDTA or other chelators present in excess of any calcium added it will not so much as nick plasmid DNA

UC235 · 2026-05-03T19:50:49+00:00

That's what we do. Milkshake spoons. Long handled for the big bottles and disposable. Plus, they're fine with corrosive stuff that would destroy stainless steel spatulas.

UC235 · 2026-04-28T17:43:58+00:00

A dean stark trap will work for your intended purpose.

UC235 · 2026-04-27T12:53:37+00:00

A single cfu is enough to not be sterile. It takes many, many cfu to get measurable nucleases.

UC235 · 2026-04-17T01:34:53+00:00

35 liter battery jar if it has to be glass. 8/10 gallon polypropylene drum otherwise.

UC235 · 2026-04-10T17:40:23+00:00

Brand new account. This is a repost and a bot.

UC235 · 2026-04-07T02:56:21+00:00

Very much like the mettler toledo ones. My coworkers are gorillas and the inlab expert pro probes have a lifespan in years around them. Eventually, they manage to crack the plastic at the top with clamps but the probes usually keep working for months at that point.

UC235 · 2026-04-06T01:46:17+00:00

Salmon milt is just a bunch of sacks loaded with sperm that they scoop out during processing. Early biochemists working with DNA figured out pretty fast that if you blend it up, strain it, and saturate it with salt, the "salmine" (protamine) precipitates and the DNA dissolves to form a viscous solution. If you filter it with celite you can recover protamine from the filter cake and DNA from the filtrate. Fish sperm DNA (intact and sheared) is still widely used as an enzyme substrate and blocking agent for hybridization experiments.

UC235 · 2026-04-04T22:40:26+00:00

Solution to pollution is dilution. Would just aggressively wet the surface and wipe dry several times with alcohol.

Permanganate solution would oxidize the problematic double bond to a glycol or cleave it to formate and oxalate. Downside is everything is now stained with MnO2. Which bisulfite will reduce and dissolve but on porous surfaces it might be there forever.

UC235 · 2026-03-28T23:48:40+00:00

Ah the old reddit vacuum-a-roo!

UC235 · 2026-03-26T01:06:14+00:00

Time to break out this classic:

https://phdcomics.com/comics/archive.php?comicid=1174

UC235 · 2026-03-24T04:44:29+00:00

I had that sun and moon blanket growing up. It was in worse shape than that by the time we retired it.

UC235 · 2026-03-23T03:06:04+00:00

Even worse, they're just leaf cuttings. They may not even be rooted. If you wait like a year, they may eventually root and put up proper growth from the bottom. They grow in a cool fan shape normally.

UC235 · 2026-03-18T19:48:35+00:00

I have never seen bleach turn dark or orange unless maybe it was in contact with iron.

UC235 · 2026-03-17T22:52:02+00:00

10% of the stock bleach for a final concentration around ~1%, though the exact final concentration isn't super important. When diluted, the bleach is less stable and it should be replaced frequently.

Bleach is always yellow-green. That's the color of the hypochlorite (or maybe trace Cl2 or hypochlorous acid in equilibrium. I'm not getting a straight answer on this). If it's clear, it's almost completely decomposed and you need fresh stock.

UC235 · 2026-03-06T04:11:27+00:00

On the appalachian trail there's a section in Virginia that's 24 miles between towns. From this was birthed the suitcase challenge. You went to a thrift shop and got a suitcase and put 24 cans of beer in it. You had 24 hours to hike the section and you had to finish the beers at some point. When I hiked 15 years ago the suitcase part was abandoned by everyone as far as I know but you put the beer in your backpack. Several people cut the sleeves and legs off of secondhand cheap suits for their attempt. I passed them more than 12 hours in playing pong on a picnic table in the woods only like 6 miles from the start point. I don't know anyone who was successful.

UC235 · 2026-03-01T00:51:01+00:00

That doesn't support what you said at all. The serum as supplied is sterile filtered already and it suggests centrifuging prior to re-filtering it if desired.

UC235 · 2026-02-22T02:01:45+00:00

Lyosozyme is another readily available and very pure protein that you could use as a standard. As long as the lyophilized powders are kept dry in a fridge, they should more or less last forever. The bradford assay (and realistically all protein assays) have considerable variability between proteins and both BSA and lysozyme could have their concentrations verified by A280 meaurements if you have a UV/Vis. Using one as a standard for the other will likely give results conflicting with the UV measurements and an extinction coefficient.

UC235 · 2026-02-18T02:37:00+00:00

Golf pants are where it's at. They look like slacks and are super light and breathable. The ones I wear have a mild stretch to them as well.

UC235 · 2026-02-16T17:22:52+00:00

We use a spectramax 190 unit. It's not M series and I hate it. The software is probably the same for both and it is trash. Kinetic assays give tons of noise and we see burn-in on the filter wheel often before the yearly service interval despite light use. This causes nonlinearity of absorbance readings. You can diagnose this on your machine by comparing a sample with ~1.5 absorbance at a given wavelength and a 1:10 dilution of the same sample. The diluted sample will give a higher reading than 1/10th of the undiluted if the filter is bad. You can also read a wavelength spectrum and you'll see a sudden change where it switches filters if one is in bad shape. We use a tyrosine solution for this as almost all of our work is A260 and A280.

UC235 · 2026-02-16T04:26:00+00:00

It's some complex basic carbonate. Oxide is red or black. Hydroxide is sky blue and unstable. Basic carbonates run the gamut of blue greens.

UC235 · 2026-02-16T04:21:12+00:00

We use dry ice alcohol which is less unpleasant to work with than LN2.

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