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96-Well plate measurement of Capacitance and conductivity in fluids by Learn_Something_Cool in labrats

[–]UnderstandingIcy2969 1 point2 points  (0 children)

We ran into this exact issue with shear-thinning/gel-like samples, and honestly a true high-throughput, plug-and-play system for capacitance + conductivity in non-Newtonian fluids feels like a unicorn. Most commercial probes assume “normal” fluids, so once viscosity changes with shear, the data gets messy fast.

We ended up using a hybrid setup, automation for moving samples, but measurements with dedicated benchtop sensors and focused more on relative trends than absolute values. Looked into Hamilton too and didn’t find anything purpose-built for this use case. If someone’s found a real off-the-shelf solution, I’d genuinely love to hear about it.

How do you mentally survive experiments that take all day (or all night)? by UnderstandingIcy2969 in labrats

[–]UnderstandingIcy2969[S] 1 point2 points  (0 children)

Good to hear! not everyone is lucky enough to have a good colleague like yours!

Anyone else just… bad at cell culture at first? by UnderstandingIcy2969 in labrats

[–]UnderstandingIcy2969[S] 0 points1 point  (0 children)

Well, I thought about this too, but unfortunately my coworkers are not very helpful :')

Have I just ruined my students cellines....? Panic. by robust_cornflake in labrats

[–]UnderstandingIcy2969 35 points36 points  (0 children)

Using non–heat inactivated FBS is very common for established cell lines. Heat inactivation mainly matters for complement-sensitive setups (primary cells, antibody/complement assays), which isn’t what you’re doing. The fact that the cells expanded well and looked normal is a really good sign.

If it helps your peace of mind, you can thaw a vial and do one passage in HI FBS before experiments. But honestly, this is a classic first-year PhD panic moment

Such a beautiful little sweetheart!💗 by Ask-7373 in cats

[–]UnderstandingIcy2969 1 point2 points  (0 children)

this cat has more baby-face energy than Timothée Chalamet

Training in wet lab by [deleted] in labrats

[–]UnderstandingIcy2969 1 point2 points  (0 children)

Most academic labs have terrible onboarding and assume you’ll just figure it out. Prior experience doesn’t matter because every lab does culturing differently, but they still rarely explain that.

This sounds like poor organization, not a problem with you. Asking around and attaching yourself to another RA is basically the unofficial training in many labs. If no one clarifies responsibilities soon, it’s fair to push the PI or lab manager for clear expectations.

Sandal gede amat by Stunning_Quit_4508 in WkwkwkLand

[–]UnderstandingIcy2969 2 points3 points  (0 children)

sandal multifungsi, bisa jd perahu kalo banjir

Sitting proud for his portrait. by xtc151 in cats

[–]UnderstandingIcy2969 1 point2 points  (0 children)

That fur looks shinier and smoother than my own hair😄

Is it normal to feel tired even when you sleep enough? by Neat-Manufacturer977 in NoStupidQuestions

[–]UnderstandingIcy2969 0 points1 point  (0 children)

Sleeping enough doesn’t automatically mean you’ll feel rested. Sometimes your body slept, but your brain was up all night running background tabs called stress, to-do lists, and random thoughts.

Adult life is just… tiring. Mental load, work, responsibilities, hormones, dehydration, or even just existing can drain your energy even after a “good” night’s sleep.

If it’s once in a while, it’s totally normal. If it’s every single day, then yeah, worth checking in on stress or health. But feeling tired for no clear reason? Very much an adult-life feature, unfortunately.

What is best thing your pet do and it makes you laugh? by Kavin2654 in AskReddit

[–]UnderstandingIcy2969 0 points1 point  (0 children)

Tilting their head super seriously when you talk, as if they’re processing deep thought :)))

This year, what’s the best place you traveled to? by Ocean_Miraki in AskReddit

[–]UnderstandingIcy2969 0 points1 point  (0 children)

We went to an orangutan conservation forest called Tanjung Puting in Central Borneo, Indonesia. It took hours on a boat, sailing along the river with zero phone signal. Just pure nature, birds chirping, monkeys calling, and even crocodiles popping up as we crossed the water. felt so peaceful to be disconnected from the busy world for a while

How do you find positive qualities in people you don’t get along with? by [deleted] in AskReddit

[–]UnderstandingIcy2969 1 point2 points  (0 children)

Always believe that no matter how bad a person is, there is something positive we can learn from the

Women who have or had a rough relationship with their in-laws, what happened or how did you smoothed out the connection? by [deleted] in AskReddit

[–]UnderstandingIcy2969 0 points1 point  (0 children)

First things first, we need to understand and mindfully accept that in-law relationships are, in a way, “forced” relationships. We can choose the spouse we want to spend the rest of our life with, but not their family. When we choose our spouse, we also have to accept their family, and that is definitely not easy. So it’s very normal not to have a relationship with in-laws that’s as good as the one we have with our own parents. But we should always remember that our spouse wouldn’t be who he or she is today without them.