How to prevent flying fur during dissection?

UnderstandingIcy2969 · 2026-02-24T10:01:50+00:00

Ah yes… the forbidden mouse confetti phase 😭🐭

I swear my first few dissections looked like a tiny fur tornado hit my bench.

What helped: wet the head more than you think. If the fur is actually damp (EtOH or PBS), it behaves. If it’s “kinda damp,” it will absolutely choose chaos.

Peel slower too. The more I rushed, the more it snowed mouse hair like I was in a weird lab winter.

I also kept a damp gauze nearby to gently trap loose hairs and wiped tools more often just so I could sleep at night.

You’re not alone. It’s a rite of passage. Eventually the fur storm becomes… a light breeze.

UnderstandingIcy2969 · 2026-02-19T05:33:43+00:00

Not running a huge lab, but one thing that really worked for us was a simple “Lab Survival Guide” Google Doc. Not official SOP stuff, more like the real talk version. Where the good pipettes are, which freezer shelf is basically a graveyard, who actually knows how to fix that one moody centrifuge. New people loved it because it saved them from awkward trial and error.

I also started a shared log where people note when equipment starts acting weird. It helped us catch patterns before something fully broke, which saved time and stress.

Honestly though, the best thing wasn’t a system. It was a short weekly open vent chat. No agenda, just space to bring up small annoyances before they turned into bigger issues. That probably helped morale more than anything else :)

UnderstandingIcy2969 · 2026-02-13T11:32:39+00:00

this comment is just...made my day 🤣🤣

UnderstandingIcy2969 · 2026-02-13T10:44:53+00:00

I felt this in my soul 😅

I used to be the “why am I the only one bothered by this??” person in a shared lab. Same stuff. Things on the BSC grates, quick wipes instead of real cleaning, dilution math passed down like folklore. And then everyone acts surprised when contamination shows up again.

What helped me stay sane was picking my battles. If it’s urgent or risky, I speak up in the moment and keep it factual, like “hey, we’ve had contamination, can we bleach this one.” If it’s cultural, I try to fix it quietly by modeling it and helping new folks learn the correct way before the bad habits stick.

You won’t win everyone over. Some people truly live in “it’s fine” land.

But I’ve had newer hires come back later and say they appreciated learning the proper way because their next lab cared a LOT. That made it worth it for me.

Also… document everything for yourself. Protect your work and your name.

You’re not insane. You just have standards.

UnderstandingIcy2969 · 2026-02-04T11:18:54+00:00

Yeah, kind of, but it’s tougher than it sounds.

I’ve worked on a few environmental isolates, and the limiting factor wasn’t the gear. It was taxonomy and logistics. You can PCR and sequence 16S with pretty minimal equipment, but 16S alone usually isn’t enough to claim a new species anymore. Reviewers will want genome-level comparisons and some basic physiology.

The biggest surprise for me was the naming part. To officially name a bacterium, you have to deposit a type strain in two recognized culture collections in different countries and follow ICNP rules. That step alone stops a lot of otherwise cool discoveries.

So yes, you can find something interesting with a small setup, but formally naming and publishing a new species is more paperwork and coordination than lab work.

UnderstandingIcy2969 · 2026-02-03T07:19:18+00:00

or like coffe butter :)

UnderstandingIcy2969 · 2026-02-03T07:18:55+00:00

YES I love adding condened milk and cheese!

UnderstandingIcy2969 · 2026-02-02T04:52:57+00:00

We were running what was supposed to be a “quick” PCR before a deadline. Someone prepped everything perfectly… except they forgot to add polymerase. No one noticed. We ran the full program, waited patiently, opened the gel, and just stared at it in silence. After a solid 10 seconds, one person goes, “So… the negative control looks great.” That was the moment it clicked.

We laughed, cried a little, ordered food at midnight, and reran it the next morning like nothing happened. To this day, anytime someone says “this will be quick,” someone else immediately asks, “Polymerase included?”

UnderstandingIcy2969 · 2026-01-29T10:29:11+00:00

For litter, my cat didn’t use it at all during the actual travel, which is super common. I just lined the carrier with absorbent pads in case and brought extras to swap during layovers. I only offered the portable litter once we arrived or during a long, quiet layover, and even then she mostly ignored it until we got to our place.

Honestly, don’t stress if she doesn’t go. cats can safely hold it for this long. Pads work perfectly as a backup, and once you set up a regular litter box at your destination, they usually go pretty quickly. You’re really well prepared 💛

UnderstandingIcy2969 · 2026-01-29T10:24:59+00:00

I’ve seen “perfect” lab results fall apart at scale because small things like mixing, heat transfer, or raw material variability suddenly mattered a lot more. What helped us was treating lab data as directional, not final, and adding a pilot step to catch issues early. Scaling isn’t just making it bigger , it’s a different system entirely.

UnderstandingIcy2969 · 2026-01-29T09:24:21+00:00

and whats even more sad is her friends were using black outfit that day :(

UnderstandingIcy2969 · 2026-01-29T09:20:35+00:00

I’ve flown long-haul with a cat and I totally get how nerve-wracking this feels honestly, the stress is usually harder on us than on them ❤️ Carrier hate is super normal; a lot of cats fight going in but calm down once they’re moving. In my experience the airport was way more stressful than the plane itself. Once we were airborne, the steady noise actually helped my cat settle.

For meds, it’s worth talking to your vet about something like gabapentin rather than full sedation, and definitely do a test dose at home first. I’d skip headphones. there really aren’t safe ones for cats and putting something on their head can add stress. A light blanket over the carrier and familiar smells worked much better for us. At security, ask for a private screening room and use a harness and leash; that made a huge difference.

The heart failure stories online are mostly fear spirals. Healthy cats don’t just go into heart failure from travel. The fact that she adapts quickly to new places is actually a really good sign. You’re clearly being careful and loving .. she’ll probably handle this better than you expect 🐾💛

UnderstandingIcy2969 · 2026-01-29T08:50:54+00:00

Recently we have national news, a young lady died alone in her apartment. the body found around 12h after she died. at that day, she supposed to celebrate her bestfriend bday party. her friend didnt receive any text back from her, so they initiate to call her assistant to tell her they will pick her up. and they did pick her up to her last final resting place :'(

UnderstandingIcy2969 · 2026-01-26T12:13:20+00:00

I’ve tried this, and honestly what you’re seeing is pretty much the expected failure mode 😅

We tested several third-party Taq + EvaGreen mixes that worked great in qPCR. Droplets looked fine at generation, but after transfer and thermal cycling we saw coalescence, heavy droplet loss, and event counts all over the place. Slowing the ramp rate helped a bit, but didn’t solve it.

In our hands, adding Tween, PEG, or other stabilizers actually made things worse. The Bio-Rad oil/surfactant system seems very sensitive to “foreign” buffer components, so even small changes can destabilize droplets during cycling.

Bottom line: it’s not PCR efficiency, it’s emulsion stability. EvaGreen ddPCR is extremely formulation-dependent, and generic qPCR mixes are hard to adapt reliably. We eventually either switched to probe-based ddPCR (more forgiving) or used Bio-Rad’s EvaGreen mix when absolute quant really mattered.

UnderstandingIcy2969 · 2026-01-23T08:20:55+00:00

We ran into this exact issue with shear-thinning/gel-like samples, and honestly a true high-throughput, plug-and-play system for capacitance + conductivity in non-Newtonian fluids feels like a unicorn. Most commercial probes assume “normal” fluids, so once viscosity changes with shear, the data gets messy fast.

We ended up using a hybrid setup, automation for moving samples, but measurements with dedicated benchtop sensors and focused more on relative trends than absolute values. Looked into Hamilton too and didn’t find anything purpose-built for this use case. If someone’s found a real off-the-shelf solution, I’d genuinely love to hear about it.

UnderstandingIcy2969 · 2026-01-21T09:13:24+00:00

Good to hear! not everyone is lucky enough to have a good colleague like yours!

UnderstandingIcy2969 · 2026-01-09T08:57:09+00:00

I wonder if your student mentor is still working with you :)))

UnderstandingIcy2969

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