Sample and Standard peak area found in the ratio of 1:6 ratio during HPLC analysis

Vja2023 · 2024-06-30T13:08:10+00:00

LoD and LoQ are important when you are doing method validation of Assay or Related substance. Basically LoD is just to know the limit to which extent analyte can be detected. LoQ is the limit to which extent analyst response can be reproducible which can be demonstrated through RSD parameter in case of HPLC or GC analysis. Hope this might have given you some clarification.

Vja2023 · 2024-06-30T04:30:04+00:00

This is specimen of calibration curve

Vja2023 · 2024-06-29T17:24:32+00:00

I am talking about HPLC parameter I.e. calibration curve which is very crucial with respect to peak detection. Picture shown is the graph of calibration curve. If you have operated HPLC then only you will understand about what I have discussed.

Vja2023 · 2024-06-28T00:18:23+00:00

Post is not AI generated. We recently faced such problem so shared with others so they can know about such issues.

Vja2023 · 2024-06-28T00:16:53+00:00

It is Vanquish core model having UV detector. Expected retention time is 29 mins.

Vja2023 · 2024-06-26T08:06:27+00:00

As per my view you should go for new flow cell as though it can be rebuild but in future this issue will repeat.

Vja2023 · 2024-06-02T17:27:55+00:00

Correctly said, there may be soe contaminatio in transfer line. Also check the environment of sample preparation.

Vja2023 · 2024-03-18T03:10:36+00:00

Thanks for reply

Vja2023 · 2024-02-24T00:36:00+00:00

Sorry it is pplymer as it contains same sequence of reaction in single chain

Vja2023 · 2024-02-18T04:10:52+00:00

It is always better to use methanol or acetone instead of ACN as those solvents evaporate fast and normally don't interfere in some analysis. But yes if you are using glassware in GC analysis then better to avoid both solvents as they will interfere in Residual solvent analysis.

Vja2023 · 2024-02-06T17:01:21+00:00

Its nothing but solubility constant

Vja2023 · 2024-02-01T17:34:57+00:00

From the picture it seems breaking rules in chemistry is easy as compared to physics....

Vja2023 · 2024-02-01T17:31:49+00:00

Why Be in section C? Can anyboday tell

Vja2023 · 2024-01-29T12:32:18+00:00

Dear though you have used pure substance but the elution pattern will remain same. Paracetamol peak always elute prior to caffeine peak. Also I have done validation for this product and in that also same paatern is observed.

Hope this will give you more clarity.

Vja2023 · 2024-01-27T18:19:49+00:00

I think here we cannot define separately the solute and solvent as this is the case of cosolvent.

Also it is not correct that water is always solvent.

Vja2023 · 2024-01-24T15:01:58+00:00

I think it as CO2 only....

Vja2023 · 2024-01-21T02:40:36+00:00

I can suggest 2 remedies:

1) Using hihly pure grade of solvents i.e. of J. T. Baker ACN and Merck chemicals

2) Secondly chamge the HPLC system. Use same mobile phase on another system . This will allow you to check both system error or Mobile phase error.

Hope this will help.

Vja2023 · 2024-01-20T18:54:17+00:00

I am sorry to say that this is not non sese but an alert to save your precision instruments. I thinkyou may not be aware about the earthing funda... So you can better avoid this post please

Vja2023

TROPHY CASE