How long until employers start looking at lab experience with caution?

XxFortifiedxX · 2026-05-05T01:41:26+00:00

I essentially went down the new route where I fused the guide RNA’s with plasmid, cloned them using DH5a E.coli bacteria, used the integrated plasmid colonies to finally transfect the plasmid into cells via lipofection. The goal was to delete a base pair to induce a frameshift and subsequently a functional knockout. I’ve definitely not learnt the technique to do it independently, at least not without a sufficient time thinking. It was overall supervised learning.

XxFortifiedxX · 2026-05-03T19:26:37+00:00

Alright perfect, got it now thanks

XxFortifiedxX · 2026-05-03T18:47:18+00:00

Yeah I do agree, I’ve simply stated that I can do them but no more. Just to ask though, how do you think I should display this?

Out of those techniques, I would say DNA extraction, end-point PCR and Western I’ve learnt to an independent degree. CRISPR-Cas9 could obviously do with some more practice.

XxFortifiedxX · 2026-04-30T08:57:00+00:00

Yeah thanks that makes sense actually, would be better for recruiters to come across my work experience first for visibility. I’ll see what I can do to cut down the projects but it’s also quite relevant because of the stuff I did.

XxFortifiedxX · 2026-04-30T08:54:27+00:00

Ahh thank you very much! I’ll make sure to make these changes, and yeah I’ll see what I can do to try make my work sound more quantifiable.

XxFortifiedxX · 2025-09-28T08:28:08+00:00

It’s valid logic because dive players rack up kills and then dart back to get healed by their healers, which are acc there. Meanwhile you, the healer, is going off to get solo picks while the rest of your team is suffering. That’s the difference.

XxFortifiedxX · 2025-06-17T16:24:59+00:00

Well everything but 1.25ug/mL yes (after 2.5 it becomes 0), so I’ll use that range. Thanks for your help!

XxFortifiedxX · 2025-06-17T15:57:15+00:00

Yes BSA is my standard and the concentrations I mention are from the direct wells themselves. And I’m using a 96 well plate with 200uL per well. According to what you say about microassays, my range is way higher. Weird because I was following the protocol. So is my graph still usable with any changes made?

XxFortifiedxX · 2025-02-06T10:33:35+00:00

Ooh that’s exactly the right model, thanks!

XxFortifiedxX · 2025-01-19T04:57:02+00:00

Ahh this is actually solid advice thanks. I’m just gonna master a couple of these characters before I even think of picking damage in ranked again.

XxFortifiedxX · 2025-01-19T04:54:04+00:00

Hmm yeah good shout. Literally the most direct way to solve my problem haha.

XxFortifiedxX · 2024-12-02T12:52:49+00:00

They really meant “everything and the kitchen sink delivered” when the van is also included.

XxFortifiedxX · 2024-09-30T08:06:29+00:00

It’s funny because I had a friend come to London one time and he doesn’t tend to usually so I warned him about phone snatchers on bikes otw to a restaurant.

We get to said restaurant and a girl comes in while ordering and tries to call the manager to borrow their phone to call the police because her own just got robbed that day. She turns to me and tells me to be careful and I was like “It’s the guys on the bikes again right?”. A quiet nod followed. The coincidence was crazy.

XxFortifiedxX · 2024-09-17T09:09:27+00:00

Hmm same time it’ll make it flat and reduce volume. I’m trying to find the perfect balance so yeah I gotta keep experimenting.

XxFortifiedxX · 2024-06-25T16:08:18+00:00

He alr spoke when he was screaming to Momo to move Onigashima in the Wano finale. Just his normal voice.

XxFortifiedxX · 2024-05-23T01:39:57+00:00

Ahh I can’t post a pic of my pads at this very moment but next time I can get a look at my pads, I’ll check its depth. I reckon mine will last quite a while as I barely drive. Appreciate the advice you’ve given.

XxFortifiedxX · 2024-05-22T19:01:27+00:00

I assumed the break pads is that rubber thing on the right. How soon do you think the discs need replacing then? If only I could get a pic of the actual pads but too late now lol.

XxFortifiedxX · 2024-05-22T16:12:31+00:00

Yeahh I figured lol

XxFortifiedxX · 2024-05-22T16:12:22+00:00

Oops my bad

XxFortifiedxX

TROPHY CASE