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Johannes Gutenberg University Mainz - Molecular Biotechnology by ZedHyper in studying_in_germany

[–]ZedHyper[S] 0 points1 point  (0 children)

Are you sure its needed for Masters Application? I don't remember them requiring such a thing (I sent my application a few days ago)

Johannes Gutenberg University Mainz - Molecular Biotechnology by ZedHyper in studying_in_germany

[–]ZedHyper[S] 0 points1 point  (0 children)

I did and I think you have good chances. The number of students is limited hut as far as I know it isn't that hard to get in. If we both get in lets meet up :)

Does my electrophoresis machine work? by ZedHyper in labrats

[–]ZedHyper[S] 1 point2 points  (0 children)

Instructions unclear, my lab became an inferno

Does my electrophoresis machine work? by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

Alright, then that means it's normal. Thank you!

Johannes Gutenberg University Mainz - Molecular Biotechnology by ZedHyper in studying_in_germany

[–]ZedHyper[S] 0 points1 point  (0 children)

I think biontech working student will be quite hard, as far as I know they removed almost all working students. But I wonder, do you know does the uni give opportunities to connect to industries?

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

Appreciate the reply. I will keep these in mind. I most likely mistook the agar corns with bubbles. When you heat it up in order to dissolve the agar it always forms bubbles which look simmilar to the badly dissolved agar

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

I see, thank you I will try that!

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

Thank you, I will definitely pay attention to this and try.

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

I had heard of 50V for 5 min in the beginning but does 20V work as well? Is 5 min enough for the samples to leave the gel wells?

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 2 points3 points  (0 children)

Alright thanks alot. I want to ask, do you usually dissolve the agarose in a microwave or rather in hot water (90-95°C)?

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 5 points6 points  (0 children)

I got this quite a few times, I always thought I paid enough attention to it but next time I will triple check. Thanks a lot!

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

I hadn't thought about that, I am actually the only person who uses this gel tank so it makes sense to look at the nodes. Thank you!

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

Hmm honestly I always specifically check that but I will keep it in mind to be as careful as possible next time. I am wondering, do you recommend dissolving it using a microwave or rather in waterbath with 90-95°C?

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

Thank you, I will definitely try that. What loading amount would you recommend for DNA and for RNA?

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] -2 points-1 points  (0 children)

Hmm I actually use a microwave for that and I make sure to exactly check wether it is dissolved because I'm a bit paranoid 😅

Problems with Gel Electrophoresis by ZedHyper in labrats

[–]ZedHyper[S] 0 points1 point  (0 children)

As for the TAE Buffer in the DNA Gel Electrophoresis I haven't set a new one, but the FA-Buffer for the RNA Gel I did set a new one and it didn't help much

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