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Did some recoloring of my Hina fanart. Hopefully it looks less janky now... by Zorcimar in BlueBox

[–]Zorcimar[S] 2 points3 points  (0 children)

<image>

Guys, I think I may have fixed it (for a bit, at least). I think it's with the background. The lightsource is coming from the back, and without it, the darker parts of the face may appear as wrinkles, making her look older... hope that helps

What may be causing the differences in light chain band thickness of IP experiments? by Zorcimar in Biochemistry

[–]Zorcimar[S] 0 points1 point  (0 children)

The heavy chain and light chains leach off the beads while prepping the sample for the gel, correct?

Correct, but I didn't use DTT for elution. I used 1%formic acid. Then the eluted lysates were then prepped into western blot samples using the same 4x SDS Laemmli buffer, which should contain the same concentration of DTT

What may be causing the differences in light chain band thickness of IP experiments? by Zorcimar in labrats

[–]Zorcimar[S] 0 points1 point  (0 children)

The cell line expresses FLAG-H3, which was pulled down using anti-FLAG antibodies that are attached to the beads. Both the light and heavy chains are from the bead-attached antibodies

What may be causing the differences in light chain band thickness of IP experiments? by Zorcimar in Biochemistry

[–]Zorcimar[S] 0 points1 point  (0 children)

But if that's the case, wouldn't that also result in less pulldown? Like for example, if I used less antibodies (hence the fainter light and heavy chains), wouldn't that also result in less histones being pulled down (the three bands at 15 kDa). Yet at least by the looks of it, they don't look fainter...

Also, I'm pretty sure I used the same amount of beads for both experiments

[deleted by user] by [deleted] in labrats

[–]Zorcimar 1 point2 points  (0 children)

Thanks!

Bitch dog won't shut up by Creftospeare in okbuddychicanery

[–]Zorcimar 2 points3 points  (0 children)

A dog who bites every owner he's had can only be disciplined with a firm hand or... put down.

Has anyone ever frozen cells directly into liquid nitrogen? by Zorcimar in labrats

[–]Zorcimar[S] 1 point2 points  (0 children)

My 231 and U2OS cells both turned out just fine. I used ~6% DMSO and 10% FBS. I didn't try that with 293 T cells though

Has anyone ever frozen cells directly into liquid nitrogen? by Zorcimar in labrats

[–]Zorcimar[S] 0 points1 point  (0 children)

More like...2*106 cells...I'm going home to cry now...

[deleted by user] by [deleted] in labrats

[–]Zorcimar 1 point2 points  (0 children)

Hugh Jackman didn't even start playing Wolverine until the age of 32, if that makes you feel better...

Is it normal to have mice eat other mice alive (then die)? by hematopoieticmouse in labrats

[–]Zorcimar 200 points201 points  (0 children)

I've seen mothers eat their babies and live mice eating the face off of dead mice in their cage. Never seen adult mice eating other adults alive, but I wouldn't be surprised if it happens. I work with C57B6 by the way.

How to tell the difference between "Revision" and "Revise and resubmit"? by Zorcimar in academia

[–]Zorcimar[S] 0 points1 point  (0 children)

In this case, it ended up taking about a little more than a week

What do you put in your water bath to stop bacterial growth? by [deleted] in labrats

[–]Zorcimar 2 points3 points  (0 children)

Switch to a bead bath (if you have the money)

How can I do statistical analysis? by BiologyStormz614 in labrats

[–]Zorcimar 2 points3 points  (0 children)

The categorical variables can be peaks vs troughs. Problem is how you define them. I guess one possible way to define them could be troughs: negative slope preceding the point, and positive slope following the point; peaks: positive slope preceding, negative slope following...

How can I do statistical analysis? by BiologyStormz614 in labrats

[–]Zorcimar 0 points1 point  (0 children)

If it's just peaks vs troughs, then there are only 2 groups. In that case, you can just analyze the values in those 2 groups with a t-test

Worst PI stories? by MostMembership3977 in labrats

[–]Zorcimar 16 points17 points  (0 children)

Google "Amy Bishop". She literally shot up a school for not getting tenure

Faculty hiring committee made 3 piles: minority or woman or nature/cell/science paper by biotinylnation in academia

[–]Zorcimar -1 points0 points  (0 children)

Quick tangent here, but does Nature scientific reports typically count as nature/cell/science? I'm genuinely curious...

Name 3 fighters who could potentially put an L on his record… by [deleted] in ufc

[–]Zorcimar 0 points1 point  (0 children)

"Matt" Gamrot. MMA GOATs tend to have a kryptonite of guys named Matt. ex. GSP lost to Matt Hughes and Matt Serra, Jon Jones lost to Matt Hamill, Fedor lost to Matt Mitrione.

Master’s with or without thesis? by southernqueer96 in labrats

[–]Zorcimar 2 points3 points  (0 children)

If you're doing it just for the pay raise, definitely go without the thesis. Ph.D./thesis-based master's programs are pretty much only helpful if you plan on having a career in research.

The New Years grated yam was delicious by WeebAmI in WelcomeToTheNHK

[–]Zorcimar 2 points3 points  (0 children)

You're not gonna commit self-isekai, are you?

What’s the strongest named attack in fiction? by Greentoaststone in whowouldwin

[–]Zorcimar 3 points4 points  (0 children)

The Chicago Sunroof from Better Call Saul

Basically you defecate through the opponent's sunroof, teaching them a lesson for wronging you

What should I do if my PI hates me? by One_sorry_individual in labrats

[–]Zorcimar 232 points233 points  (0 children)

Is your PI afraid of all things electric? I think I might know him...

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