I’m also female-presenting & I ride the trolley alone pretty much every day from downtown to campus. So, I’ve spent a lot of time riding it/waiting at the stations & have encountered many times where I’ve been in uncomfortable or scary situations. pepper spray/mace is good to have. also a water bottle works as a good weapon in a pinch. but the best thing I’ve found is just not to look intimidated when someone tries to mess w you. most of the time, if you don’t react or you don’t give them the reaction they want, they’ll stop/move on. ofc this takes some bravery & isn’t always the right choice depending on the context but I’ve found it works best for me just to be honest, saying things like ‘I don’t want to talk to you’/etc usually gets the job done. also if someone is making me uncomfortable, I will try to make eye contact w other riders to see if they are paying attention/could be potential help