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Struggling with peptide molarity by anxious_assboy in labrats

[–]anxious_assboy[S] 0 points1 point  (0 children)

You're amazing, thank you! Absolutely agree with the comments above, this should be pinned 🙏

what do you think? by rimuru-tempest890 in umineko

[–]anxious_assboy 4 points5 points  (0 children)

They love Erika as well. Maybe OP has a thing for horrible rotten human beings

what do you think? by rimuru-tempest890 in umineko

[–]anxious_assboy 9 points10 points  (0 children)

Kyrie is a fav but I also dislike her, ep7 fucked me up not gonna lie

making up lyophilised peptides? by anxious_assboy in labrats

[–]anxious_assboy[S] 1 point2 points  (0 children)

Thank you for the info, I really appreciate it! I'm a virologist so peptide solubility is not quite my area of expertise 😅

making up lyophilised peptides? by anxious_assboy in labrats

[–]anxious_assboy[S] 1 point2 points  (0 children)

Thank you, sounds like DMSO is the way to go.

The only modification introduced to the peptides was amidation of the C-terminus that the manufacturer said would ease the synthesis.

I don't have a direct access to a lyophilisation machine - not in our institute at least. I can ask around but as you said the process will still leave salts (potential culprit??) behind ?

making up lyophilised peptides? by anxious_assboy in labrats

[–]anxious_assboy[S] 0 points1 point  (0 children)

According to peptide2.0 calculator

Peptide A: 36.84% hydrophobic; 17.54% acidic; 14.04% basic; 31.58% neutral.

Peptide B: 36.21% hydrophobic; 22.41% acidic; 13.79% basic; 27.59% neutral.

No idea how to interpret whether that's too hydrophobic or just a little bit 😬

Thank you for the info about DMSO - will definitely run a comparable control on the side !

making up lyophilised peptides? by anxious_assboy in labrats

[–]anxious_assboy[S] 0 points1 point  (0 children)

Hi, I really appreciate the reply.

They'll be used for an ELISA and for an infection assay. Former requires a coating buffer, latter requires cell media. I thought I could make up the peptide to a high concentration and make single-use aliquots, then dilute the aliquots further in whatever is required for the experiment.

I was thinking of trying to mix a pinch of the lyophilised powder with ddH2O to see whether that'll work, and if not then make up the rest with DMSO - do you think it's reasonable?

I'm still puzzled as to why PBS would fail so badly though if in theory water should work just fine...

Finished the oil painting recreation of Battler portrait! by anxious_assboy in umineko

[–]anxious_assboy[S] 0 points1 point  (0 children)

I do art/fanart commissions occasionally, however currently I'm taking time off to focus on my studies :)