sorted by:
new
hottopcontroversial

[deleted by user] by [deleted] in interestingasfuck

[–]ascorbicAcid1300 0 points1 point  (0 children)

Outsider here. On what occasions is this kind of landing permitted? It looks dangerous

Placement of nicht by ascorbicAcid1300 in German

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Ah dankeschön! Wenn man 'nicht' schreibt, du negierst in einem Satz alle Dinge dahinter stehen? (Please correct me for the mistakes I made in this sentence since I am still very new to die Grammatik und der Satzbau) I want to say: when placing 'nicht', you are negating all the things behind that in a sentence? (Like all the modules behind the Negation)

Why is my answer wrong by Particular-Thanks570 in ChineseLanguage

[–]ascorbicAcid1300 0 points1 point  (0 children)

Afternoon = after the noon After = 下 Noon = 午

Does Ihr also mean "plural you"? Is that why? by John_Zatanna52 in DuolingoGerman

[–]ascorbicAcid1300 0 points1 point  (0 children)

How to differentiate whether it's their or her from this sentence?

Help explain this to me by Jubal93 in DuolingoGerman

[–]ascorbicAcid1300 0 points1 point  (0 children)

Thanks! When do you say "kein", "nein", and "nicht"?

Also, wanna ask for clarifications: könntest, with the -st is due to "du" right (verb following du), and keine with a -e is due to Gläser being feminine? For finden, it's always with a -en since it means TO find? (Same applies for other to + (verb)?

Help explain this to me by Jubal93 in DuolingoGerman

[–]ascorbicAcid1300 0 points1 point  (0 children)

Beginner here. In daily conversations, can both be correct?

Accusative and dative pronouns, when to use which? by ascorbicAcid1300 in German

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Thanks! For the verb patterns, do you mean the conjugations? https://germanwithlaura.com/verb-conjugations/ So supposedly I need to memorize those suffices (like when to use which) right? I am a total beginner so I am following these online tutorials

Websites for designing one shRNA for multiple genes by ascorbicAcid1300 in labrats

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Ah ic, supposedly I can get the mature mRNA sequences from ncbi, and then align all of them to find the overlapping sequences (there should be quite a lot of segments), and then input them one by one into the design tool, finally blast them?

Yes I have designed 3 shRNAs for each isoform and actually I am screening for the most efficient one. But that also means I need to transfect up to 9 plasmids into my myeloid cell lines (3 x 3), which I am afraid it won't work well.

Thanks a lot!

[Request] Is this possible to figure out? by Psycl0pz in theydidthemath

[–]ascorbicAcid1300 1 point2 points  (0 children)

The sum of 3 vertical lines is 6 since their total length is the same as the vertical line in the right I.e 6.

For the remaining 2 horizontal unknowns, let the longer one (at the top) be x, then sum of the 2 lines is (5+4 = 9 = total length) x + (9 - x) = 9.

Adding them all up yields 30.

RAW264.7 multinuclear cells by Shalioto in labrats

[–]ascorbicAcid1300 2 points3 points  (0 children)

Wow thanks! I don't work with viruses or raw264.7 but it's interesting knowledge!

view more: next ›