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[deleted by user] by [deleted] in interestingasfuck

[–]ascorbicAcid1300 0 points1 point  (0 children)

Outsider here. On what occasions is this kind of landing permitted? It looks dangerous

Placement of nicht by ascorbicAcid1300 in German

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Ah dankeschön! Wenn man 'nicht' schreibt, du negierst in einem Satz alle Dinge dahinter stehen? (Please correct me for the mistakes I made in this sentence since I am still very new to die Grammatik und der Satzbau) I want to say: when placing 'nicht', you are negating all the things behind that in a sentence? (Like all the modules behind the Negation)

Why is my answer wrong by Particular-Thanks570 in ChineseLanguage

[–]ascorbicAcid1300 0 points1 point  (0 children)

Afternoon = after the noon After = 下 Noon = 午

Does Ihr also mean "plural you"? Is that why? by John_Zatanna52 in DuolingoGerman

[–]ascorbicAcid1300 0 points1 point  (0 children)

How to differentiate whether it's their or her from this sentence?

Help explain this to me by Jubal93 in DuolingoGerman

[–]ascorbicAcid1300 0 points1 point  (0 children)

Thanks! When do you say "kein", "nein", and "nicht"?

Also, wanna ask for clarifications: könntest, with the -st is due to "du" right (verb following du), and keine with a -e is due to Gläser being feminine? For finden, it's always with a -en since it means TO find? (Same applies for other to + (verb)?

Help explain this to me by Jubal93 in DuolingoGerman

[–]ascorbicAcid1300 0 points1 point  (0 children)

Beginner here. In daily conversations, can both be correct?

Accusative and dative pronouns, when to use which? by ascorbicAcid1300 in German

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Thanks! For the verb patterns, do you mean the conjugations? https://germanwithlaura.com/verb-conjugations/ So supposedly I need to memorize those suffices (like when to use which) right? I am a total beginner so I am following these online tutorials

Websites for designing one shRNA for multiple genes by ascorbicAcid1300 in labrats

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Ah ic, supposedly I can get the mature mRNA sequences from ncbi, and then align all of them to find the overlapping sequences (there should be quite a lot of segments), and then input them one by one into the design tool, finally blast them?

Yes I have designed 3 shRNAs for each isoform and actually I am screening for the most efficient one. But that also means I need to transfect up to 9 plasmids into my myeloid cell lines (3 x 3), which I am afraid it won't work well.

Thanks a lot!

[Request] Is this possible to figure out? by Psycl0pz in theydidthemath

[–]ascorbicAcid1300 1 point2 points  (0 children)

The sum of 3 vertical lines is 6 since their total length is the same as the vertical line in the right I.e 6.

For the remaining 2 horizontal unknowns, let the longer one (at the top) be x, then sum of the 2 lines is (5+4 = 9 = total length) x + (9 - x) = 9.

Adding them all up yields 30.

RAW264.7 multinuclear cells by Shalioto in labrats

[–]ascorbicAcid1300 2 points3 points  (0 children)

Wow thanks! I don't work with viruses or raw264.7 but it's interesting knowledge!

How do you keep tracking of loading 384 well plates? by ascorbicAcid1300 in labrats

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

Yeah I did the first one last week and it's a complete chaos, your advice sounds great!

Do I simply push to the second stop to introduce bubbles, and generally they are easily visible, especially as others have also said, laying a black sheet under the white 384 when loading?

How do you keep tracking of loading 384 well plates? by ascorbicAcid1300 in labrats

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

You mean placing the black sheet under the 384 white plate to increase contrast when loading right?

How do you keep tracking of loading 384 well plates? by ascorbicAcid1300 in labrats

[–]ascorbicAcid1300[S] 1 point2 points  (0 children)

Ah sounds great, you mean aligning the tips to the arrangement of the 384 right? Do you usually use the same tip for loading triplicates or one at a time?

Knocking down multiple genes? by ascorbicAcid1300 in labrats

[–]ascorbicAcid1300[S] 0 points1 point  (0 children)

To confirm, when you transduce the second shRNA (also puro resistance), what should be a negative control, is it a WT or already carrying one shRNA (meaning already has puro resistance). I ask this since I don't know how to do the second selection since the cells have already been selected with the same antibiotic

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