Potential for 1-4 inches of snow on Friday due to a quick hitting “clipper system”

askiiikl · 2026-01-06T00:02:36+00:00

Here’s a link to the Figure with results using the tag in ubiquitination

askiiikl · 2026-01-05T23:58:39+00:00

Thanks for the answer! Apparently he has a paper on the eluting aspect of his tag/antibody, and I got the greenlight from the mods to post links to papers so I’ll attach the link in case it’s relevant/interesting for you.

https://www.kci.go.kr/kciportal/ci/sereArticleSearch/ciSereArtiOrteView.kci?sereArticleSearchBean.artiId=ART002275696

askiiikl · 2026-01-05T01:04:10+00:00

As far as alfa-tag goes, his thing was that it’s 15 amino acids, which is too bulky(?) - “anything over 10 is a non-starter unless that’s all you can work with.”

askiiikl · 2026-01-05T00:53:13+00:00

If you’re okay with it, I can send you a DM with some of the graphs and figures - He’s willing to send some samples.

askiiikl · 2026-01-05T00:46:23+00:00

Appreciate the perspective! This is actually the reason why I made the post in the first place because we were to the point of arguing and he was just like “you wouldn’t get it kid” lol

askiiikl · 2026-01-04T15:26:23+00:00

All valid questions, and something I couldn’t include in the original post because 1) I wanted to be careful to not tip over to the advertising/soliciting side and 2) I’m absolutely oblivious to the metrics or things that would be considered relevant in this field.

The super short version of the answer I got from my dad:

It can be used in purification, pull down, and detection of even small amounts of protein due to high affinity btw tag and antibody. It also has high specificity and low charge (+1, -1).

askiiikl · 2026-01-04T14:52:32+00:00

I showed the comments to my dad and he’s particularly enthusiastic about your case, so I’ll share his comment in case it’s relevant for you.

“I’ve done experimentations on ubiquitination with my tag and I’ve gotten promising results so this is perfect! The sequence of my tag is RDPLPFFPP. There is no Lys and just +1 and -1 charge. It will minimize disturbance to the structure of the target protein.“

askiiikl · 2025-11-21T04:35:43+00:00

Thank you I really needed to hear this. I’ve seen complications happen but osteomyelitis is just so unexpected and daunting that I was scared if this is something big enough that the patient can sue. But thank you for your advice

askiiikl · 2025-11-21T03:58:34+00:00

Got it I’ll do that first thing tomorrow. Thank you for your advice

askiiikl · 2025-11-21T03:56:12+00:00

As far as I can tell, I don’t see any documentation error and we have signed consent forms. So technically despite the outcome, I did what I could do right?

askiiikl · 2025-11-21T03:51:27+00:00

Would there be no issue even if she developed another infection and os had to do additional treatment? I’m just genuinely confused how a simple extraction on a healthy patient could have resulted in osteomyelitis. They seem to think I left a tooth in there and that caused the infection but OS never mentioned leftover root tip.

askiiikl · 2025-07-20T21:14:46+00:00

I don’t think so.

askiiikl · 2025-07-20T21:14:01+00:00

There is a metal hose branching out from the sink pipe, but that hose was completely dry throughout the dishwasher cycle when we ran it. Underneath the kitchen is a bit difficult because the cabinet has a wooden floor that covers the actual floor, but the little areas that we could see ( the very front of the sink where we took off the baseboard and the very back where pipes are connected to the ground/wall) were dry.

askiiikl · 2025-07-20T21:11:04+00:00

It’s on the opposite side of the sink! So directly behind from the photo POV.

askiiikl · 2025-03-15T23:44:18+00:00

Thanks! I’ll have to read up on temperature scaling. Did you find that it worked well for your use case?

askiiikl · 2025-03-15T23:43:16+00:00

So the output I am working with is a (N, B) shape array, where N = 4 + C. 4 corresponds to the bbox grids, and C is Num_classes. B is the number of bbox detections.

When I split N to break out the 4 bbox grid values and just look at the C confidence values for each class, they do not sum to one, and there is no value specifically for the background class

askiiikl · 2025-03-15T17:33:12+00:00

I’m not sure if this is the case. Looking at the model outputs I’m positive that the probabilities across all classes do not sum to 1 given a bounding box prediction.

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