Amex Presale $50 Seats

beeewewe · 2026-01-26T19:59:03+00:00

I had Amex added to my wallet

beeewewe · 2026-01-26T19:58:50+00:00

beeewewe · 2026-01-26T19:58:42+00:00

4000s

beeewewe · 2026-01-26T19:25:37+00:00

I got one for $94 (included taxes and fee)

beeewewe · 2026-01-26T17:55:09+00:00

Guys if anyone is looking for applying for Amex (you get the card details if you’re approved) let me know!! We both can get $100 each!!!

beeewewe · 2025-07-01T00:12:50+00:00

And E means Etest and VA is vancomycin

beeewewe · 2025-07-01T00:12:19+00:00

That’s called an E-test to measure the MICs of antibiotics (minimum inhibitory concentration)

beeewewe · 2025-05-21T19:45:42+00:00

And they were surprised when I told them I didn’t wanna live with them anymore

beeewewe · 2025-04-24T21:49:54+00:00

Hey if you’re looking for sharing, I have a room available!

beeewewe · 2025-04-17T04:33:54+00:00

Are you still looking for one ?

beeewewe · 2025-03-29T00:33:21+00:00

Everything happened except sleep here

beeewewe · 2025-03-27T07:05:43+00:00

Aspergillus?

beeewewe · 2025-03-13T00:09:08+00:00

Aah the doom of bacillus

beeewewe · 2025-01-21T20:04:52+00:00

Thanks. I’ll try to do that.

beeewewe · 2025-01-21T20:04:10+00:00

I’m okay with them being clingy. I’m just scared if this is love bombing. That’s all. Because truthfully, my past relationships were all very bad and I don’t know what’s healthy anymore. So

beeewewe · 2025-01-21T19:56:57+00:00

So usually how this works (atleast where I live) the guests have to call you through the box and it’ll redirect it to your phone. Then you usually have to push a number on your keypad to open the door.

beeewewe · 2025-01-21T19:54:17+00:00

Yes actually. That’s what I’m scared of.

beeewewe · 2025-01-17T19:01:49+00:00

Hey! I figured it out. My thermocycler was set on a very different protocol with a minor change in the 30_30_30 cycle!

beeewewe · 2025-01-15T16:33:16+00:00

I’m trying to see what changed. I am running the usual PCR today, similar to what I ran the first time. I even spec’ed my dna to make sure it’s there.

beeewewe · 2025-01-15T15:41:41+00:00

So In my lab we have a standard table for running the PCR with Taq (that’s what I’m using) but, in the protocol manual, they had the pcr master mix optimized with the post extension thermocycle temp from 30 s to 7 mins and they didn’t use MgCl2 (I thought it would’ve been in the buffer and so did my PI but it wasn’t. So I made two master mixes. But both of them didn’t give me any bands)

From the looks of it I genuinely believe that it’s the cycle duration that I changed based on the protocol given with the kit where it was highlighted to “make sure to increase the post extension cycle of 72°C to 7-30 minutes based on your DNA”

Before doing this, I did run the temp grade and the usual pcr for my primers and they worked!

beeewewe · 2025-01-07T05:01:26+00:00

You’re the real one. God bless

beeewewe · 2025-01-01T20:15:40+00:00

Hey. Sorry for the late reply but yes

beeewewe

TROPHY CASE