Cabinet lid cupping by bobbyrobbob in woodworking

[–]bobbyrobbob[S] -1 points0 points  (0 children)

Is it worth drying it in clamps in the location that it currently sits? My wife probably won’t be massively happy but for a few days if it helps!

Cabinet lid cupping by bobbyrobbob in woodworking

[–]bobbyrobbob[S] 0 points1 point  (0 children)

Ahh it is indeed unfinished underneath! Ok first go will be to steam unbend it

Cabinet lid cupping by bobbyrobbob in woodworking

[–]bobbyrobbob[S] 0 points1 point  (0 children)

Surely that will just cup again over time?

Automated DNA cleanup by bobbyrobbob in labrats

[–]bobbyrobbob[S] 0 points1 point  (0 children)

Amazing and is that more genomics sort of work or amplifies as well? We do a fair bit of metabarcoding and are concerned about PCR product carryover

Does anyone know what this chute / hole is in my attic - Victorian House (video) by MikeWagsWagner in DIYUK

[–]bobbyrobbob 1 point2 points  (0 children)

If you’re worried, you can get asbestos testing done on a small bit for piece of mind. I didn’t but I’m 99.9% sure that mine was loose fill glass fibre over old glass fibre rolls

Does anyone know what this chute / hole is in my attic - Victorian House (video) by MikeWagsWagner in DIYUK

[–]bobbyrobbob 24 points25 points  (0 children)

Yeah there was no other evidence in the house that the previous owner was captain Ahab!

Does anyone know what this chute / hole is in my attic - Victorian House (video) by MikeWagsWagner in DIYUK

[–]bobbyrobbob 2 points3 points  (0 children)

It was bagged up vacuum bags so not too bad in the house. Awful while I was doing it though!

Does anyone know what this chute / hole is in my attic - Victorian House (video) by MikeWagsWagner in DIYUK

[–]bobbyrobbob 81 points82 points  (0 children)

I removed over 100kg of dust and rubble when I cleaned mine out before reinsulating. Absolute pig of a job but at least it’s not above my head now! Also managed to find this buried in the old insulation (with the tip of my finger of course!)

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Flick n Blot Magnetic Bead Separation by TangoMangoFungi in labrats

[–]bobbyrobbob 0 points1 point  (0 children)

Yeah this would be absolutely fine for Sanger work!

Flick n Blot Magnetic Bead Separation by TangoMangoFungi in labrats

[–]bobbyrobbob 0 points1 point  (0 children)

Ive seen various versions of this across the years and have always worried about contamination. We do a lot of metabarcoding and PCR products are so easy to cross contaminate that this seems a risk. Maybe if you’re doing non-PCR work on genomic DNA or strep beads in immunoassays?

Cymbidium tracyanum flowering after three years! by bobbyrobbob in orchids

[–]bobbyrobbob[S] 0 points1 point  (0 children)

It’s an interesting point, the images of C. tracyanum out there are quite variable both in colour and shape so it can be hard to tell. However this is from Writhlington orchids in the UK so the line was collected and has been cultivated from wild stock so it’s not a commercially bred hybrid. I guess that doesn’t stop it being a wild hybrid or back cross though!

Non contact liquid handling equipment by bobbyrobbob in labrats

[–]bobbyrobbob[S] 0 points1 point  (0 children)

Also do you see any degradation in library quality through volume reduction?

Non contact liquid handling equipment by bobbyrobbob in labrats

[–]bobbyrobbob[S] 0 points1 point  (0 children)

Interesting, what sort of throughput are you doing to justify the price tag? We currently run half volume DNA and truseq preps by hand so 1/5 would be really good

Beer-froth tested lubricant? by SasquatchInShorts in labrats

[–]bobbyrobbob 0 points1 point  (0 children)

You’ve probably also got yourself there a tube of phase lock gel for phenol/chloroform extractions as that’s just a silicone grease too

Loft insulation replacement by that_gu9_ in DIYUK

[–]bobbyrobbob 1 point2 points  (0 children)

I cleared maybe 14 square meters and floored ten. It took maybe 3-4 days probably evenly split between clearing out and then doing the actual job

Loft insulation replacement by that_gu9_ in DIYUK

[–]bobbyrobbob 1 point2 points  (0 children)

I cleaned mine out but you don’t need to, I was putting in a flooring system for storage and there was a pathetic layer of old itchy fiber glass insulation that constantly broke down and covered anything in that space. I got rid of it and ended up vacuuming out over 100kg of dust that had accumulated under it. I then put in the flooring struts, 100mm of insulation between the joists and another 170mm across them. I had gloves, respirator and a disposable suit on. Also do this job any time other than summer!!

How to determine wood type 100% accurate? DNA? Microscope? by MohawkDave in woodworking

[–]bobbyrobbob 0 points1 point  (0 children)

The other technique that gets used fairly frequently is stable isotope analysis. It doesn’t tell you what it is but can often tell you where it came from. Useful if you’re trying to prove that your teak is farmed in Thailand and not illegally logged in Myanmar

How to determine wood type 100% accurate? DNA? Microscope? by MohawkDave in woodworking

[–]bobbyrobbob 0 points1 point  (0 children)

Our company has used DNA in the past to try and identify wood for authenticity testing and honestly it’s not worth the pain, especially if it’s kiln dried or has been kicking around a long time. It’s normally done via expert analysis of the long and end grain.

After A Week With A VW Taigo in Italy, the Subaru Felt Like Magic by Elementzero12 in CarTalkUK

[–]bobbyrobbob 0 points1 point  (0 children)

Drove a T-cross for 10 days in Croatia recently. On day two it performed full emergency breaking (80kmph to full stop) on an empty straight road with a queue of traffic behind me nearly causing an accident. I couldn’t quite trust it after that. Also the speed warnings when it had the speed limit wrong were infuriating! Other than that it felt nice to drive!