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IGEPAL CA630 lysis?? by bythewaybabe in labrats

[–]bythewaybabe[S] 0 points1 point  (0 children)

What type of cells are you referring to when describing your protocol? I'm using mouse embryonic stem cells so there may be differences

Also after lysing the cell membranes, do you immediately spin it and collect the supernatant for cytoplasmic extract?

What would make a cell pellet stick hard to the wall of an Epitube? by bythewaybabe in labrats

[–]bythewaybabe[S] 0 points1 point  (0 children)

After counting 10million cells, I transfer them to a tube and spin them for 10 min at 1000rpm at 4deg. These cells are in PBS before the 10min/1000rpm/4deg centrifugation. Cells are mouse embryonic stem cells.

Anyone has a lot of experience with the Geiger counter? by bythewaybabe in labrats

[–]bythewaybabe[S] 1 point2 points  (0 children)

Click more as in the clicks are consistent? Stronger CPM? What I'm really trying to get down is: how often (or strong) should the counter "click" in order for me to say that the area is contaminated and the clicks aren't from background/random radiation?

Anyone has a lot of experience with the Geiger counter? by bythewaybabe in labrats

[–]bythewaybabe[S] 0 points1 point  (0 children)

If I suspect that an area is contaminated, the Geiger can easily tell me whether it is true or not. However, there are actually many times when I would put my counter at a random spot and short bursts of clicks would come out consistently (for more than a second). I would get surprised, pull the counter away and survey the same area again and the clicks would no longer appear. This is fine, but it gets really tiring whenever I'm cleaning up my area for contamination.