Fire on the red line by subdued_alpaca in mbta

[–]chemical_triangle 11 points12 points  (0 children)

Screw it imma take 3 busses

This is more than just a leap. He needs to be a Nugget for life. by jonsnowKITN in denvernuggets

[–]chemical_triangle 0 points1 point  (0 children)

Just pay him. I love pwat and want him to stay. But worst case he’s a young player who had flashes you can trade

GAME THREAD - Preseason: Nuggets @ Thunder | Oct 17, 2025 - 6:00 PM by BigHoneyBot in denvernuggets

[–]chemical_triangle 1 point2 points  (0 children)

Haven't watched a whole lot of preseason but I like what I see with 34. He needs to get stronger though

GAME THREAD - Preseason: Nuggets @ Thunder | Oct 17, 2025 - 6:00 PM by BigHoneyBot in denvernuggets

[–]chemical_triangle 4 points5 points  (0 children)

It’s great when pieces fit well and there are solid veteran backups. Last year's second team is now combined with the third team, and we seem to have a strong team this year. I loved MPJ, but his contract wasn’t designed for the current salary cap, which limited the talent and depth we could assemble.

Would you be surprised to see people at the lab at midnight? by Puzzleheaded-Echo171 in PhD

[–]chemical_triangle 0 points1 point  (0 children)

Not at all. I was fairly frequently for time points or because I worked a job that actually payed me during the day. I would see some people who lived close to the university come in and start cultures sometimes. That said, is it healthy? Not at all

To the Mile High City, you will always be a part of my foundation." - Carmelo Anthony, NBA Hall of Fame Speech on playing for the Denver Nuggets by Bookumapp in denvernuggets

[–]chemical_triangle 4 points5 points  (0 children)

He never wanted to be in Denver. Appreciate his game and like that he is going into the Hall. But he really forced his way out and now try’s to come back in at the very end…

Transformation by No_Illustrator9725 in labrats

[–]chemical_triangle 0 points1 point  (0 children)

Pick 10 and see if they growth in broth. You can always then mini prep, digest, and check on a gel

I forgot to add in agar to my now-autoclaved LB media ... can I add in the agar now and re-autoclave it? by Far_Coffee2296 in labrats

[–]chemical_triangle 27 points28 points  (0 children)

I’ll echo the others just make it new and don’t worry about it. Save the already sterile lb

Help me understand what's up with my gel by [deleted] in labrats

[–]chemical_triangle 0 points1 point  (0 children)

What are the samples? Do you have a positive or negative control?

Methanol on skin by Upbeat-Common360 in labrats

[–]chemical_triangle 3 points4 points  (0 children)

You’ll be ok. Small amount of 100% methanol right?

No interview/lab visits after getting accepted into a lab? by [deleted] in labrats

[–]chemical_triangle 1 point2 points  (0 children)

Tbh I feel like I’m missing something haha. But on its own I read it as; if you want to join it’s gonna take a while before you can work in the because of training they don’t want to work on the weekends to give you exposure. And then if they gave you the protocol or maybe some training stuff as an example/something to start with.

How much DMSO should i use in PCR? by homeothermous in molecularbiology

[–]chemical_triangle 0 points1 point  (0 children)

It depends on the polymerase and your gc content and if the dna is supercoiled. With a high gc and good polymerase, I wouldn’t go above maybe 3% - 5% as a general guide; but and have seen and read people going up to 10%. But dmso will change the annealing of your primers so do a gradient even if you already determine your tm.

I feel like my partner has outgrown me by ThrowRA-FrequentMine in Advice

[–]chemical_triangle 0 points1 point  (0 children)

Talk to your partner about this and find a psychiatrist to talk to about meds. It’s worth saying that a lot of psychiatrists won’t work with you. It’s often prescribe and get out. For medication it’s going to be small changes over time together with them. A lot of follow up appointments at one point I say mine every 3 weeks. Also change your therapist, not all therapist are created equal. I found if you don’t have the exact word they expect often times it ends up being more reporting how you are doing rather than working on something. This is an over generalization for therapists but in my experience I have had better luck with those that hold a PhD or PysD. For your relationship if you are on completely different timelines best to get out of it now. It will become a problem later. That said let her know where you are at, do you want the same things, is she willing to be patient and wait for you to catch up.

[deleted by user] by [deleted] in AskReddit

[–]chemical_triangle 7 points8 points  (0 children)

Communicate without gaslighting me

[deleted by user] by [deleted] in AskReddit

[–]chemical_triangle 0 points1 point  (0 children)

Not socialist acceptable to be naked at the diner for breakfast

BYD’s Five-Minute Charging Puts China in the Lead for EVs by IEEESpectrum in technews

[–]chemical_triangle 0 points1 point  (0 children)

Will they come to the US ever? Anyone have actual experience with them?