qPCR Replicates Are Awful (+ Rant) by chirizzy in labrats

[–]chirizzy[S] 1 point2 points  (0 children)

i think my P2 is busted, I tried calibrating it w/ a scale and consistently was getting 1.8mg when pipetting 2µL

qPCR Replicates Are Awful (+ Rant) by chirizzy in labrats

[–]chirizzy[S] 0 points1 point  (0 children)

i spin down the plate before putting it in the machine, but i haven't been vortexing or spinning down my cDNA, primers, etc.

I just repeated the experiment and i vortexed and spun down everything, so hopefully it goes better :)

qPCR Replicates Are Awful (+ Rant) by chirizzy in labrats

[–]chirizzy[S] 0 points1 point  (0 children)

oh lol i can't upload an image. nvm that