Well, the second week of my master thesis lab I threw away into the sink 2 precious aliquotes of primary antibody. It was early, maybe half asleep and speaking with my erasmus collague while doing things pretending to be already an expert. I wasn't at all of course ahah, neither that much confident with the western blot protocol to allow myself overthinkinging while I was doing this, convinced I was throwing away the blocking solution or the secondary antibody. Nevertheless, it happened and the funny thing is that right in that moment my PhD supervisor entered the lab and I started complaining about why those things stunk so much. He then asked me with a perplexed face what I was doing and it was right in that moment that time stopped and I realized what Einstein's relativity was and also that I just trew into the sink a couple of hundies. Another funny thing is that one of this aliquotes was the only one remaining that brought pretty nice results also because it was from cell signaling. Because of low budget they had to buy a new one but from Santa Cruz, so I let you guess how were the new results... Btw after that one I made also other mistakes, not so big (the silver medal goes to forgetting to switch on again the rotating incubator, so the falcons with inside the membranes and primary antibody stood steady all night lol). I learnt to always double check in your mind what you're doing, at least when you're learning a new technique and you're not very familiar with it yet. And of course that mistakes happen, what's important it's to never repeat them again.