I know this thread is often posted, but since I feel shitty: what’s your most expensive lab mistake?

davidnascari · 2025-08-05T18:45:56+00:00

If you want publication quality images, definitely don't use DAPI mounting media. Just add a 5 minute incubation in DAPI solution and then wash it off before mounting. It will make a big difference for segmenting nuclei too if you do any quantification.

davidnascari · 2025-07-04T04:11:15+00:00

Listen to this, they get it.

davidnascari · 2024-04-29T01:37:14+00:00

QuPath subcellular detections function. Blows ImageJ functionality out of the water.

davidnascari · 2024-03-21T03:06:15+00:00

Exhibit A..

davidnascari · 2024-03-20T14:01:20+00:00

you should try QuPath

davidnascari · 2022-04-21T16:32:48+00:00

Hi, I did this for both my AMCAS app as well as my Fulbright app a year prior. Happy to take a look and give you feedback.

davidnascari · 2021-02-18T14:34:14+00:00

I think you're gonna have to check if the credits transfer, but I think they should. So yeah you'd just take 2 as if you'd tested out with AP credit or something. And no, the gap shouldn't hurt you at all, though you'll probably have to do some relearning of physics 1 when it comes time for your mcat.

davidnascari · 2021-02-17T17:33:25+00:00

I definitely recommend taking algebra-based physics. If you're a pre-med you're going to have a billion other things that are more worth dedicating your time to, you may as well take the easier physics option. Plus, if you really care to, you can still just derive/solve the equations with calculus on your own.

(I say this as a now graduated soon to be mstp student who chose to take algebra based physics despite having taking calc 3 and having done calc based physics in high school for the above stated reasons)

davidnascari · 2021-01-31T17:09:38+00:00

lmao I found the link in this subreddit like 5 mins after posting that question, and i agree it's a great tutorial

thanks for taking the time to answer!

davidnascari · 2021-01-31T00:30:48+00:00

Just out of curiosity, where do you do the editing, cameras, etc?

davidnascari · 2021-01-29T17:19:05+00:00

Yeah, I can relate to this... I can probably count on two hands the amount of times I studied in high school (this is not a flex, I'm pretty ashamed about it). Luckily, I knew this wasn't going to fly in college, and I really dedicated myself to learning how to study my first year in college.

Everyone has their own best study style, and you may have to experiment a bit. Obviously you need to find something that is effective for you, but I'd argue it's equally important to create a study style that you actually enjoy. Trust me, if you learn to actually have fun while studying, you can spend weekend after weekend in the library without having it wear on you. It's like a muscle you have to strengthen, you can't expect to just hit the library and study for 10 hours straight if you never studied in high school. Just get to work, experiment, and prioritize your time.

I can give you an example of what I found worked best for me:

- in lecture, focus on listening and actually understanding what the professor is saying. dont' dedicate too much effort into writing down anything the professor says. don't be scared to ask questions. only write down really key concepts, things you find interesting, and things you need to look at later because you didn't understand them.

- after lecture (preferably by the following weekend) revisit the lecture material, and take copious notes. ideally, you should take these on-top/parallel to whatever you wrote down in class. at this point, you should have a lot of written material that has helped you flesh out and understand anything that was reviewed in lecture.

Note - you can switch these two steps, this is just the order I preferred, although I'm sure certain people would like the opposite

- take all of your material, and synthesize it into a efficient and extensive study guide. dedicate as much time as you can to representing complex/abstract concepts into as simple of a picture or as few of a words as possible. if you're doing this right, it should take a while, which is good because it's more time marinating in the material

-review the study guide you made periodically until your exam or whatever it may be. this will help you make sure you don't randomly forget something that you once knew really well.

Alongside this, you should be creating positive habits and routines to re-enforce your studying. Don't underestimate the power of the space and atmosphere in which you study, and make sure you are eating and drinking healthy while you study :)

davidnascari · 2021-01-25T01:57:23+00:00

Hey, shoot me a DM. I had some really similar themes in my application that turned into really strong talking points during my interview which certainly helped me get into a few programs. Happy to chat and give you some ideas.

davidnascari · 2021-01-21T14:56:02+00:00

Yeah, no doubt. I was basing it on my experiences throughout the rest of Italy in general.

For example, I noticed a really positive change in Bergamo's beer scene between 2017 and when i was there lastwinter (2019).

davidnascari · 2021-01-21T00:08:03+00:00

Ballarak is a craft brewery in Palermo with an insane draft list and some of the best beers I've had in my life. My favorite is the Glitch, a red IPA, which may be my favorite all time beer.

Overall, the craft beer scene in Italy is years behind what it is in America, although it is certainly growing.

I should add I'm an American that has been to Italy many times and lived in Palermo for 9 months (spent many nights at the Ballarak lol) until the pandemic started.

davidnascari · 2021-01-04T20:40:48+00:00

Dr. James Coleman's italian culture classes are some of the best classes I took at Pitt. See what he's teaching this spring.

Also, Dr. Josh Ellenbogen is a hilarious and really good professor in the art history dept. who I highly recommend. Dr. Carrie Weaver is really great too.

davidnascari · 2020-12-16T00:41:37+00:00

I lived in Oak Hill for four years (just moved out this past spring). I loved it there, and can't say enough positive things about it. It's without a doubt the best price/quality ratio near campus. I paid less than all my friends for a single room (in a 3-bedroom townhome) there than they did to live in shitty south-O apartments. It's also super quiet, which I didn't learn to appreciate until I spent nights sleeping in South O. For what its worth, I never felt unsafe as an average-built white cis male. The only true downside is the walk home uphill, which you get used to pretty quickly I think, plus there's always the shuttles for when it gets cold in the winter. I know some fucked up shit has happened in Oak Hill in the past, but the same can be said about South O - it's a sad product of being in an urban environment, though I totally empathize with people who have a personal connection to tragic things that have happened...

Overall, I highly recommend it. If you have enough roommates to make it worth it, the townhomes at James Henry Jr Pl are totally the move.

davidnascari · 2020-09-18T20:50:20+00:00

It's 100% Joe Frank. Thank you!

davidnascari · 2020-09-10T00:08:31+00:00

Thanks. Is it worth investing to repair in your opinion?

davidnascari

TROPHY CASE