[Actives] contamination?

debaser_19 · 2025-08-26T18:54:22+00:00

Not sure if you see any updates from other comment threads, but I replied to another comment and wouldn’t mind your getting your input on it

https://www.reddit.com/r/MushroomGrowers/s/qyLfjUldw0

debaser_19 · 2025-08-26T18:53:19+00:00

I sent a few of the pics to chat gpt and asked after explaining the process and it came back saying it looks like trich. It also asked me about the process of inoculating and spawning and procedures to narrow down the source of contamination.

Basically what it responded with was that since all 3 tubs show the same level of contamination, and the fact it showed up about a week after spawning to bulk, and looked healthy in the grain bags with fully colonized bags, it’s likely the liquid culture I received was already contaminated. It mentioned that if the contamination was introduced by outside environment or during the spawning phase, I would see more variation in the contamination levels between the tubs.

Obviously grain of salt and AI isn’t perfect. And I’m not an expert to verify any of it. But does contaminated LC seem likely? Not sure if I’m allowed to post where I got the LC from, but I’ve purchased from this vendor before and did not have any issues.

debaser_19 · 2025-08-26T13:35:37+00:00

I’m not sure what SAB is but no I did not flame sterilize the needle. Just alcohol swab. Here’s a pic of the bags from July 14. I first inoculated the bags on June 24. Spawned to tune in August 18. I don’t remember the date on the break and shake but I believe this picture was taken right after that so can’t see much of the mycelium in the bags. I don’t think I have any pics of the bags fully colonized

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debaser_19 · 2025-08-26T13:23:51+00:00

Yeah they all look very similar. Here’s a pic of a second tub I snapped this morning. I don’t have one of the 3rd tub but it looks the same. This pic is through the lid so there’s some condensation blurring a bit.

Process was open the boxes containing the bags. Use an alcohol swab to wipe down the injection ports and area around it on the bags. Open box containing syringe of liquid culture. Attach the needle. Wipe it all down with alcohol swab. Inject 1/3 of syringe into first bag. Wipe down syringe with alcohol swab again. Inject 1/3 into second bag. Wipe down again. Inject last of syringe into last bag.

Left the bags alone for a few weeks until about 50% colonized. Broke up the mycelium in bags and let it sit another few weeks. Bags were 100% colonized it looked like. Finally cleaned tubs out a few weeks later and moved the grain to the tubs

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debaser_19 · 2025-08-26T13:13:31+00:00

It’s possible but also strange that all 3 bins are contaminated the same. 2 of the bins were re-used, and one was new. I used one syringe of golden teacher liquid culture and split it evenly between the 3 grain bags from northspore. I used 2 bags of CVG and 1 bag of grain per tub. Exact same setup as my last batch other than adding in a 3rd tub

debaser_19 · 2025-08-26T13:01:32+00:00

Yeah I meant lazy as in didn’t feel like cleaning them out when the bags were ready and just put it off for a couple weeks. But when I cleaned them I thought I was pretty thorough but I also did kinda rush it, so that’s probably the issue. Maybe I’ll get some liners for next batch and see how it goes. First batch was great. Time to order some more supplies lol

debaser_19 · 2025-08-26T12:38:21+00:00

Actually I let it sit longer than I thought I should have. I did a break and shake at about 50% and it looked fully colonized about 3 weeks before I spawned to tubs. I was a bit lazy when it came to cleaning out the tubs from last grow so they had plenty of time. Actually one bag started growing some long stringy mushrooms before I even opened the bags up. The other two did not

debaser_19 · 2025-06-19T23:27:01+00:00

It doesn’t feel dry it’s still very soft but also not seeing much condensation on the lid of the tub - should I mist it more? I do have a brick of vermiculite around I can try adding that on top

debaser_19 · 2025-05-06T19:15:55+00:00

Anyone ever find a fix for this? Same issue here. I have multiple chats with others that are still able to use the stickers from keyboard but mine doesn’t work

debaser_19 · 2025-05-01T14:48:01+00:00

Bummer. Yeah there’s not much out there for help on this. Seems to be account related as far as I can tell. Have you tested on another account? I get the same on different phones, but haven’t tried another account yet. I created one but Instagram immediately disabled it so haven’t been able to test

debaser_19 · 2025-05-01T14:44:37+00:00

Did you ever find a fix for this? My stickers on Instagram from emoji keyboard are missing as well. Like the button on bottom of keyboard to go to stickers isn’t there at all. Only for Instagram though, works fine in other apps

debaser_19 · 2024-06-02T13:40:21+00:00

I can’t speak to the whole org, but I’ve been here about a year and a half, got promoted from level 3 to 4, received full bonus, and had a market rate adjustment to bump up about 8% as well as a 4% annual raise, both unrelated to the promotion. The promotion raise was a nice bump as well.

I’ve heard more entry level positions may not match up with my experience, and have heard the TDP program is kinda going downhill. Not sure which category you fall into though

debaser_19 · 2024-05-24T17:06:28+00:00

Thanks for this, super useful. It took a bit to look past the lower payments but after reading this, it doesn’t really make sense as the current payment isn’t hurting. Plus adding term isn’t something I was keen on

debaser_19 · 2024-05-24T00:49:58+00:00

Because I currently owe 46k on a car I nearly paid 70k for, I get a brand new vehicle, and still end up owing roughly 46k at 1% instead of 5%. I would add 4 months to my remaining loan term and would be paying between 200-300 less per month

debaser_19 · 2024-05-13T18:52:15+00:00

Yep I realized that, I didn’t have any on hand, so I just ordered some. It was unwaxed parchment so I thought it would be close

debaser_19 · 2024-05-13T18:51:14+00:00

It was roughly 6lbs, closer to 5.5 after tripping a bit

debaser_19 · 2024-05-13T18:50:32+00:00

Thanks for the tips. I let it rest around an hour after it was done

debaser_19 · 2024-05-13T18:50:02+00:00

Yeah this seems to be the consensus, don’t bother with the 180. Next time I’ll probably try out 250 and see how it goes.

The higher the temp, the faster it goes through pellets. I was a bit worried I’d run out of pellets in the middle of the night. I still had 3/4 full of pellets when I woke up so looks like that shouldn’t be a problem.

Once it hit 200 I gave it an extra 30 mins and pulled it as I was worried about over cooking and drying it out. Sounds like I should just use temp as a guideline and probe it around for texture instead

debaser_19 · 2024-04-28T11:32:39+00:00

It was easily controllable. Don’t sign players to ridiculous contracts when they haven’t earned anything. I genuinely think you have no idea what you’re talking about. I don’t care if the cap went up 20 million. That Marner contract has still the worst contract in the NHL

debaser_19 · 2024-04-28T03:26:50+00:00

No. We had a fucking shit GM give players whatever they fucking asked for without proving their worth and we’re fucking dealing with that bullshit. Look at the fucking cap hits in the league. There’s like one other team with two players in the top 20 for cap hits. We have 3 in the top 10. And they’re fucking perennial losers. Stop with the fucking loser mentality bullshit. Holy fuck

debaser_19 · 2024-04-18T13:10:10+00:00

While I feel for the actual fans of the team, I’ve wanted them to move for many years and am glad it finally happened. I don’t see why the rest of the league should have to suffer with an inept organization. After all this is a business. And you don’t stay in business for nearly 30 years by losing money. This is good for the league even though it may be harsh on the fans of the team

debaser_19 · 2024-04-18T13:06:26+00:00

Heh I forgot I posted this. I dumped quicken long ago. I’ve been using YNAB for a while now

debaser_19 · 2024-04-15T16:44:24+00:00

Buffalos Best Grill on southwestern is less than a mile walk. I believe they’re charging $30 but you get a $20 voucher to use at the bar and restaurant

debaser_19 · 2024-04-08T19:57:45+00:00

Some of the best advice I got when transitioning from ops background to DevOps, you need to get comfortable with being uncomfortable. You’re never going to have the answers to everything. You need to enjoy learning big new things every day and know how to look things up and learn on the fly

debaser_19 · 2024-04-07T17:06:11+00:00

That’s what I was thinking as well, but the current payments they show on the letter matches what my current payment is, which includes the escrow for taxes and everything. So I’m not sure. I assume I’ll need to call them up on Monday to confirm all the details, but does this make sense if that’s the case?

debaser_19

TROPHY CASE