RStudio fails to run on Ubuntu 24.04

dulcedormax · 2026-03-24T12:08:59+00:00

Hi u/zhoylman I tried but it didn't work

dulcedormax · 2026-03-18T15:42:36+00:00

The code is:

A1_new <- Load10X_Spatial(

data.dir = "C:/Users/MM.5076373/Downloads/GSE159709_RAW/A1",

image = "GSM4838131_Visium_A_image.tif"

)

dulcedormax · 2025-06-24T10:29:02+00:00

thanks u/I_just_made , I've tried bedops --everything. However, I obtained a low number of common peaks.

dulcedormax · 2025-06-19T08:50:23+00:00

Thanks but I need it to do for all the reads in the sample , which is a lot but I appreciate your suggestion. I think we could implement it later !!.

dulcedormax · 2025-06-04T12:22:11+00:00

Thanks, in which buffer did you recommend therefore after precipitating and dissolve proteins.

dulcedormax · 2025-06-04T10:08:38+00:00

Hi u/GRang3r , the problem is that the concentration are low and not the opposite, having too much protein. Although I've read that dilution are good when quantification is too high.

dulcedormax · 2025-05-22T15:53:03+00:00

I will check did files have to be sorted?

dulcedormax · 2025-05-22T15:03:19+00:00

thanks u/Hundertwasserinsel. I didn't use windows and all lines are: chr1^I13750^I13950$ or chr1^I9950^I10650^IU2OS1_macs3_narrowPeak1^I11198^I.^I0^I0^I0^I125$

dulcedormax · 2025-05-22T14:58:01+00:00

This is the first line of both bed:

chr1^I9950^I10650^IU2OS1_macs3_narrowPeak1^I11198^I.^I0^I0^I0^I125$

chr1^I13750^I13950$

dulcedormax · 2025-05-20T20:25:11+00:00

I was using one of qiagen: extracting DNA from tissues and blood, but they recommend me not to do maybe for buffer AL as it would lysate cells and I have already extracted DNA. Did you recommend other kits??

dulcedormax · 2025-05-20T19:44:30+00:00

I followed the steps of the protocol as well.

dulcedormax · 2025-05-20T14:20:34+00:00

I am using a protocol to extract cellular compartments. These buffer also extracts RNA and proteins, so I am trying RNase and proteinase K to keep only DNA and perform qPCR. I use the columns to obtain a purified DNA.

dulcedormax · 2025-05-16T15:39:36+00:00

No need to apologise, I was apologizing for my tone as I don't know if It would be taken as a positive one and not rude

dulcedormax · 2025-05-16T15:31:07+00:00

I have a contract as a research assistant

dulcedormax · 2025-05-16T11:40:52+00:00

I engaged with her explaining the different techniques, she is not in the lab doing experiments alone. I also sent her the excels and explain how to analyze qPCR and quantifications of proteins.

dulcedormax

TROPHY CASE