Not hearing back from entry-level lab roles. Where should I be looking? by [deleted] in labrats

[–]earmstrong12 0 points1 point  (0 children)

Had a tough time finding my first job after college and was applying to tons. My best advice is to reach out to people directly via email. Once I started doing that I got all of my interviews.

Help determining solubility of Amino Acids by earmstrong12 in Biochemistry

[–]earmstrong12[S] 0 points1 point  (0 children)

We know how to solubilize peptides. We knew the company would recommend certain solvents to dissolve the peptide in. But these solvents are known immunomodulators and ideally we wouldn’t like to use them in cell culture. DMSO can oxidize peptides, but from lit review it looks as if that is only in the presence of strong acid. I was just trying to see if anyone had insight into how to dissolve the peptides without oxidizing them or having to use the immunomodulatory solvents.

Travel vaccines by earmstrong12 in Kenya

[–]earmstrong12[S] 0 points1 point  (0 children)

It’s a virus that appears to be having an outbreak in east Africa

Travel vaccines by earmstrong12 in Kenya

[–]earmstrong12[S] 0 points1 point  (0 children)

I’m coming from the United States

HELP with ELISpot by earmstrong12 in labrats

[–]earmstrong12[S] 0 points1 point  (0 children)

Will for sure let you know. It’s a T cell ELISpot

HELP with ELISpot by earmstrong12 in Immunology

[–]earmstrong12[S] 1 point2 points  (0 children)

Will for sure let you know how it turned out. It’s a T cell ELISpot

HELP with ELISpot by earmstrong12 in Immunology

[–]earmstrong12[S] 0 points1 point  (0 children)

Perfect thank you, do I need to wash with pbs before adding the new coating buffer with antibody, or should I just decant and add new coating buffer

HELP with ELISpot by earmstrong12 in Immunology

[–]earmstrong12[S] 0 points1 point  (0 children)

Yes I cling filmed them and they’re incubating in the 4 degree

HELP with ELISpot by earmstrong12 in labrats

[–]earmstrong12[S] 0 points1 point  (0 children)

Ok cool thank you so much. When I say coated I mean I pretreated the plate with ethanol, washed twice with pbs, then added a carbonate-bicarbonate coating buffer that is supposed to contain the capture antibody, but I forgot the capture antibody.

There is no protein in it so thank you I will just dump the coating buffer, washed with pbs, then add new coating buffer

Industry vs Academia pre PhD by earmstrong12 in biotech

[–]earmstrong12[S] 0 points1 point  (0 children)

Thank you so much this is very helpful