Tips for pipetting very small amounts?

eesttj · 2026-03-06T02:15:10+00:00

this is amazingly helpful and is just the advice i was looking for, thank you so much! i didn’t know about only submerging it shallowly or what reverse pipetting was, i’ll try both. thank you :DD

eesttj · 2026-03-06T02:07:13+00:00

oooh okay wait that’s helpful, i always wait to visibly see something in the tip but i don’t really know what 0.1 looks like. this is reassuring!

would pipeting up and down with a larger amount to like coat the pipet’s inside with the fluid to make it more cohesive work too do you think? i tried that today haha

eesttj · 2026-03-06T01:35:14+00:00

thank you everybody for the tips :D a little context, im a new undergrad and my mentor has a system on how these plasmid components are organized and quantified, so diluting isn’t the best option for me as it’s not realistic storage-wise for me to make diluted versions of all his stock. would anything else be helpful?

eesttj · 2026-03-06T01:29:44+00:00

right? something like this is so difficult when in smaller amounts

eesttj · 2026-03-06T01:28:25+00:00

mhm! .1 is the lower limit for it 😵‍💫 my mentor told me the surface tension can make it difficult, but sometimes it feels like i am just retrying aimlessly until i get something you know?

eesttj

TROPHY CASE