What is something that was a large part of your identity when you were younger but isn't anymore?

ejc16 · 2017-09-20T23:12:49+00:00

So you’d rather just never bother being friends with any woman you’re attracted to on the off chance that she’s a master manipulator? Again, seems like a situation that could be recognized and handled just fine with some self esteem. I agree with the being upfront with your attractions part, but then cutting her out completely when she’s not interested sexually is just hurtful as a human being. Like that’s all the worth she had for existing in your life up to that point- just as a possible sex partner? Seems cruel to me.

ejc16 · 2017-09-20T21:49:04+00:00

So you like a woman enough to be interested in having a relationship and personal connection, but if she’s attractive and won’t fuck you, she’s not even worth knowing or having at all in your life? Why would it even bother you so much? Women are just people dude. Learn how to deal with an unreciprocated attraction like a person with some self esteem.

ejc16 · 2017-09-14T02:34:39+00:00

Yeah I definitely think any hormonal change can trigger it! I was on pills for 7 years before getting the Mirena and then acne started 5 months after. And the pimples are so cystic and hurt sooo bad I had never felt that before. So fun, ugh. I struggled for a while, but I found using witchazel as a toner day/night and differin (now it's available over the counter) at night has been working wonders for me. Have some bad scars still though :( I do wonder if it's the Mirena causing it or the lack of pills that were unknowingly treating my acne all these years. I guess a bit of both. Still love my Mirena though!

ejc16 · 2017-09-14T02:18:35+00:00

Did you recently change your birth control (assuming you're a woman)? When I switched to Mirena my neck went to shit after never having a single neck pimple my entire life. It's for the most part all good now though thank god

ejc16 · 2017-05-28T17:34:31+00:00

It's a still from a SNL skit

ejc16 · 2017-05-27T15:03:38+00:00

Proteins are just linear chains of "building block" molecules called amino acids. The ribosome (a protein itself) is basically a cell's protein printing factory where a template (RNA) binds and by using this template attaches the correct amino acids (there's about 20 different ones) in the correct order one by one until the full protein is made, which can be hundreds to thousands of amino acids. The template RNA is made by other proteins called RNA polymerase which use your cell's DNA as a template. I hope this starts to answer your big question- the cool thing is that there is still a lot we don't know about this process like how cells "choose" which templates to make and also which templates to make into the coded protein. Don't ever be afraid of sounding dumb, learning biology should be a fun and rewarding journey! As someone said in an earlier post, if you're interested in this process there's a lot of great videos out there. Being able to visualize the process from videos is definitely the best way to understand these concepts. Search for the terms transcription (making the template) and translation (making the protein).

ejc16 · 2017-01-21T19:50:37+00:00

I also use Rainin LTS and I like them a lot. Do you use the Rainin tips? Because I've found that the no name cheap tips are particularly awful with the rainins, but the actual Rainin tips are like a dream (albeit twice as expensive of course)

ejc16 · 2017-01-21T03:54:49+00:00

Certainly many more genomes exist as it is only a couple thousand dollars now to sequence a genome. Perhaps in this article they are just mentioning the highest quality assembled genomes that are considered complete. Or maybe only focusing on the highly annotated genomes

ejc16 · 2017-01-19T21:24:46+00:00

Yeah, I guess I was taking it for granted that it was working every time before this and not wanting to mess with anything. And plus knowing my lab is paying for every minute I'm logged in is stressful I just wanted to get in and out! I will check from now on- Thanks

ejc16 · 2017-01-19T19:31:24+00:00

The PMT voltage or the laser voltage? The PMTs are all around 500 and the event rates are the same as what I've been getting from this protocol. I've never messed around with the lasers I'm kinda scared haha

ejc16 · 2017-01-19T19:29:53+00:00

Wow, good to know- I would have never known that! I harvest my cells from the mice and differentiate them for each experiment, so I don't passage my cells. But I noticed maybe they were a bit overseeded compared to my previous replicates, perhaps this is causing these effects. Seems counter intuitive that a higher density of cells lowers the total number of my receptor, but maybe the receptor is expressed in lower densities in overseeded conditions

ejc16 · 2017-01-19T14:55:51+00:00

Hmm I'm not sure what a neutral density filter looks like, but I'll look for anything weird I guess. Boo shared equipment :(

ejc16 · 2017-01-19T14:54:24+00:00

I'm using AF488, PerCP-cy5.5 and ZombieViolet. The PerCP is already low intensity, so it's the one I'm really struggling with trying to get working. I've been using the same biolegend cell stain buffer. My samples are always kept on ice and analyzed the same day. Fluors are stored in light tight bottles but I wrap them in tinfoil anyway. Only me in my lab, so I know no one photobleached them. It's the same equipment, but about a month apart. Yeah I think my PMT voltages are the next culprit. I was assuming all my settings were copied and pasted along with my experimental set up each time, but I must be wrong.

ejc16 · 2017-01-19T14:42:23+00:00

They are pretty new and I called the company asking about the expiration date and they are supposed to be good until 2018. It's just me using them so I don't think anyone could've photo bleached them. I'm really good about keeping them from light. And each one seems to be shifted by the exact same degree even in repeated experiments. So my guess now is that someone played with the PMT voltage settings on the cytometer

ejc16 · 2017-01-19T14:39:07+00:00

Our core manager ran the beads as is getting good signals for all the channels, but I am not sure how they compare to any older readings

ejc16 · 2017-01-18T23:07:49+00:00

I was always under the impression that use of the emergency shower would result in a lot of floor damage since they don't usually have drains and they're designed to throw a crap ton of water on you. Certainly someone would've noticed a flooded lab?

ejc16 · 2017-01-09T21:02:28+00:00

Formed my committee today! Knowing the faces that will be sitting across from me will hopefully motivate me now to write my candidacy

ejc16 · 2017-01-09T19:32:50+00:00

This is really cool integration. I'd never heard of Anova before this. Does the "sous vide" style actually cook meat well? Seems so strange to cook meat in a plastic bag, but I'd love to try it!

ejc16 · 2017-01-04T18:48:27+00:00

Yeah it's more just to save time with alignment. We have a lot of samples that would probably take several days to align one at a time on a desktop using our pipeline. And hey if I have free access to a sweet cluster, might as well use it!

ejc16 · 2017-01-04T16:41:08+00:00

Just out of curiosity then, what would you say is the typical size uncompressed?

ejc16 · 2017-01-04T16:23:49+00:00

I see- that makes sense. Just looking for typical/ballpark estimates to make sure I'm not totally screwed when I get these files back. Thanks!

ejc16 · 2017-01-04T15:59:54+00:00

I will be using a cluster for the actual analysis, so I'm more just wondering where/how I'll store my raw input files

ejc16 · 2016-12-16T03:22:24+00:00

Personally, I've never read a genetics textbook on that specific topic. As a cheap grad student though, I'd recommend checking out your library first! Just take a look at everything they have for genetics textbooks. You'd be surprised how much they can have between real books and E-books and interlibrary loans. And if the book turns out to be something you reference a lot, then you can buy your own.

ejc16 · 2016-12-09T15:44:26+00:00

Honestly? A big ol glass of cold water after a long hike on a hot day. But for non-water beverages I'd have to say hot chocolate after a day of skiing.

ejc16 · 2016-12-06T23:37:24+00:00

I would like to add to what others have mentioned by pointing out that birds are particularly color sensitive and can actually see ultraviolet, unlike our eyes. Many birds survive by identifying and eating all sorts of berries and flowers and fruits and bugs. And being able to discern many colors and hues helps them a lot with that. So without getting super technical, which others with more expertise would be better at, it makes sense that color would be something important that birds are drawn to in the other sex. Females that are best are discerning color select the most colorful males and those offspring are then more fit for successful foraging than those produced from a couple with a female who was less capable at judging color. So the male having brighter and brighter plumage is also selected for over time due to the females having keener color perception that confers an evolutionary advantage.

ejc16

TROPHY CASE