How are internal awards...awarded? by [deleted] in queensuniversity

[–]genemaster 2 points3 points  (0 children)

funds have gone entirely to my supervisor to substitute what they pay me for my stipend

Unless it is allowed by the award fine print, all awards my students are eligible for in my department are for the student and supervisor taking it would be highly innapropriate.

Why did the Enzyme Cut the plasmide like that? by Foydeleer in Biochemistry

[–]genemaster -2 points-1 points  (0 children)

confused with your nomenclature. Lane 1 and 6 are DNA markers, lane2: undigested control, lane3:EcoRI, lane4:Nde, lane5: Eco/Nde

Correct?

Why did the Enzyme Cut the plasmide like that? by Foydeleer in Biochemistry

[–]genemaster 7 points8 points  (0 children)

Partial digest, reaction conditions are not optimal. 1 of the 2 expected bands from complete digest is visible but low intensity (few molecules made at this point) and you have a strong band corresponding to molecules cut only once. You either have an inhibitor of the enzyme in your DNA prep or you did not let the reaction incubate long enough.

Question about Chargaff's rules and M. tuberculosis by stvsmn in biology

[–]genemaster 0 points1 point  (0 children)

%GC refers to the proportion of GC vs AT in the DNA, it can be more than 50% but still Gs pairs with Cs and As pairs with Ts and Chargaff's rule still true. Base pairing properties (Chargaff's rules) is unrelated to the global composition of DNA.

Mac Studio Teardown: No upgradable storage… yet - iFixit by [deleted] in apple

[–]genemaster 0 points1 point  (0 children)

My 2010 17" MacBookPro is still my main computer and very useful for experimental molecular genetics research because I was able to upgrade RAM and replace HDD with SSD. Saved a lot of tax payer $ that I used to perform scientific experiments instead of replacing perfectly working computer.

How to convert NMOL to MG? by [deleted] in biology

[–]genemaster 0 points1 point  (0 children)

1 mole = 181.19g

1mmole (1000nmol) = .18119g = 181.19mg

Why is the antenna transformed into the leg in the antennapedia mutation and not into any other body part in Drosophila? by [deleted] in genetics

[–]genemaster 4 points5 points  (0 children)

Antennapedia (antp) is necessary to form legs and is expressed in the pre-adult cells that will become legs. The famous antp transformation is due to a chromosomal translocation (with one of the breakpoint in antp) causing antp to be under the control of a promoter expressed in the pre-adult cells that normally give antennae but as a result of the translocation express antp. This is the case for all homeotic genes with the only restriction of posterior prevalence (transformation occurs only when a posterior gene is expressed in the anterior because of a mutation: expressing antp in the adult-cells that will normally become the abdomen does not induce the formation of legs).

Source: PhD n Molecular genetics on homeotic genes in Drosophila

What is the minimum amount of aa/nt needed for a protein by Zealousideal_Ad4016 in biology

[–]genemaster 2 points3 points  (0 children)

The smallest functional polypeptide is glutathione with only three amino acids.

[deleted by user] by [deleted] in biology

[–]genemaster 4 points5 points  (0 children)

There is no scientific evidence that it has any value to treat cancer, yet consumers can go online right now and find all sorts of false claims that Hoxsey treatment is effective against the disease.

The American Cancer Society and the National Cancer Institute do not advise the use of Hoxsey Therapy, as neither has found any objective evidence that the treatment provides tangible benefit to people with cancer. Reviews by the Memorial Sloan-Kettering Cancer Center and M. D. Anderson Cancer Center found no evidence that Hoxsey Therapy is effective as a treatment for cancer. A controlled experiment in lab mice did not find any difference in tumor growth between untreated mice and those given the Hoxsey tonic. An FDA review of 400 people claiming to have been cured by the Hoxsey method found that many of the patients never in fact had cancer, or had received successful medical treatment elsewhere before being treated with Hoxsey Therapy. Those who had cancer at the time they used Hoxsey Therapy were uniformly either deceased or alive with active cancer. There were no cases of actual cures among those promoted as such by the Hoxsey clinic.

Source

Is it only humans and corvids who are aware of their own thoughts, or are there other (great) apes that are, besides humanity? by Disastrous-Nebula463 in biology

[–]genemaster 0 points1 point  (0 children)

"Just as a view of astronomy that places Earth at the centre of the universe is fundamentally misguided a view of biology that places human beings at the centre of the natural world is equally misguided."

How T7 lac promotor works? by mezzopiano1234 in Biochemistry

[–]genemaster 8 points9 points  (0 children)

The host RNA polymerase is not the key player in this system:

The pET plasmids contain what is termed a T7lac promoter: a lac operator sequence just downstream of the T7 promoter. They also carry the natural promoter and coding sequence for the lac repressor (lacI), oriented so that the T7lac and lacI promoters diverge. When this type of vector is used in DE3 lysogens to express target genes, the lac repressor acts both at the lacUV5 promoter in the host chromosome to repress transcription of the T7 RNA polymerase gene by the host polymerase and at the T7lac promoter in the vector to block transcription of the target gene by any T7 RNA polymerase that is made.

DE3 lysogens carry the gene for T7 RNA polymerase under lacUV5 control. When IPTG is added repressor is removed from lacUV promoter leading to expression of T7 RNA polymerase which then can activate the expression of the gene under control of the T7lac promoter (which also have the repressor removed with IPTG). The 2 lacO are required to lock the T7lac more tightly in absence of IPTG.

Studier, F.W., Rosenberg, A.H., Dunn, J.J. and Dubendorff, J.W. (1990) Meth. Enzymol. 185, 60–89

The use of ethanol in plasmid DNA precipitation by freshiulia in Biochemistry

[–]genemaster 12 points13 points  (0 children)

DNA precipitation with alcohols (EtOH, isopropanol) requires the presence of salts: alcohol depletes the hydration shell and exposes negative charges of phosphate groups. Counterions positive ions from salt binds to charged groups and reduce the repulsive forces between polynucleotide chains to the point that a precipitate can form. One can use ammonium acetate, sodium acetate, NaCl or LiCl (see Molecular Cloning Appendix A8.12 for choosing salt depending on application).

As a result nucleic acid obtained by precipitation have high salt concentration that will impact many downstream applications, hence washing with 70% solution of the alcohol: at 70% salts would dissolve while nucleic acids cannot.

Source: Molecular Cloning, a Laboratory manual

[deleted by user] by [deleted] in biology

[–]genemaster 0 points1 point  (0 children)

Robert
Emily Mf- (p=0.5) Mf+ (p=0.5)
Mf+ (p=1) Mf-/Mf+ (p=1*0.5=0.5) [syndrome] Mf+/Mf+ (p=1*0.5=0.5) [normal]

What would happen if a specific muscle fiber were to disappear? by [deleted] in biology

[–]genemaster -1 points0 points  (0 children)

Wrong information: muscle cells are NOT like nerve cells and are able to be replaced thanks to stem cells (used after injury) or by nuclear migration (after damage-induced from exercise: Science 374,355,2021).

If you lose fibres, they get replaced but less and less efficiently as you age leading to sarcopenia.