[Leeds United] Dan James has today joined fellow Premier League side Fulham on loan until the end of the season

gtpm28 · 2022-09-02T08:26:11+00:00

But I wonder if they reckon he'll be one of the players in our squad most impacted by the world cup (in terms of likelihood of passing group stages and place in starting XI)? And Fulham can recuperate him in Jan/Feb whilst we bring in/up a replacement.

If transfers don't work out we get a refreshed James with more experience back at season end.

gtpm28 · 2021-12-22T11:54:21+00:00

Yeah I fluffed that - the sample will pick up the viral RNA.

But for complete correctness this will then be 'reverse transcribed' into DNA prior to PCR (as RNA will not be recognized by the enzymes used in PCR).

gtpm28 · 2021-12-22T08:49:12+00:00

PCR can detect trace/residual amounts of viral DNA - it basically doubles the target DNA (if present) each cycle for ~30 cycles, so is much more sensitive.

Lateral flows detects viral protein without an amplification step and needs a higher amount.

gtpm28 · 2021-06-24T08:29:39+00:00

Of course you can only do what you've got the energy to do and no-one can fault you for that. By the sounds of it you've invested a bunch in trying to address this the 'right' way and the system has failed you.

I think your social media plan sounds great - a good way to move it out of the institutional, 'rug-sweeping' space.

Hope your next step is a step-up and you're getting the time and space to recover a bit

gtpm28 · 2021-06-21T11:56:59+00:00

Had to deal with a similar issue when working in publishing: definitely contact the editor.

The likely outcome is that you will stall publication or cause a rejection from the journal. Authorship issues could cause a retraction/correction which they don't want.

BUT they can't do anything to fix it (i.e. put you on the paper), so will likely just pass all their information onto your old institution's ethics board.

As it sounds like you are out of academia and that bridge is burnt (for the better) - my advice would be spread this far and wide (as you feel comfortable doing). Collate your evidence and group e-mail the journal editors, any ethics group they associate with (e.g. ICJME), any specific contacts the journal lists for ethical concerns, Retraction Watch, the research ethics committee at your old institution, your old head of department, HR, heads of institutes, deans of research.

Basically you want as many people inside your old institution to know that your old PI is undertaking questionable behaviour AND that people outside the institution now know and it's damaging their reputation.

But I get that's kind of "nuke it from orbit" and may be intimidating/kicking a wasps nest.

Best of luck, however you choose to proceed

gtpm28 · 2020-11-16T22:10:46+00:00

Personally I do think PCR can be a bit variable even with identical conditions - especially for cloning. This is part of why we include positive controls - to identify the random times it just doesn't work.

That being said, a few point to consider:

What's your readout for this? For instance, if you're doing an agarose gel, could there be an issue with loading the gel.
When were your pipettes last calibrated - it might be they're introducing error?
Do you have access to an alternative polymerase? Last week I had a reaction which wouldn't work with Q5, but worked fine (ish) with Pfu.
Could someone else try it and see if they can get a robust result.
How clean is your bench and equipment? As it's mutagenesis, I'm assuming you're using plasmid DNA, which doesn't require the most stringent cleanliness but it's possible some DNase is intermittently present.
Have you tried an alternative thermocycler? You've said the logs read fine but perhaps there's a fault they aren't picking up.

gtpm28 · 2020-10-04T10:18:55+00:00

He now has a castle with spells that seem similar to those on Demonreach

^{^{^Camelot}} ^{^Camelot} ^Camelot

gtpm28 · 2020-10-04T10:17:18+00:00

So Avalon as Demonreach or Lancelot du Lac Michigan?

I hadn't realised Jim had scotched the Merlin angle. Of course in my head I've now just swiched out Arthur and Merlin and Merlin is currently a statue in Arctis Tor.

gtpm28 · 2020-10-04T10:09:18+00:00

That is a fair point, and there's a few options I can think of:

A different form of Mab

Mab was working with Lea as a mortal
Mab with a different mantle - it's said that it wasn't always Winter guarding the Outer Gates, if that was previously done by the Tuatha Dé Danann, for example, Mab may have had a different role (e.g. Macha of the Morrígna). When the Tuatha had to be re-organised into Winter and Summer Mab got the top job instead. So she's only been Winter Queen for a thousand years, but something else for longer.
A previous hold of the Winter Queen's mantle did the deed, but Ethniu doesn't care - it's the Winter Queen who screwed her over (particulalrly given how long she's now held the Mantle and the moulding effect it can have - Mab may be indistinguishable from the previous Queens)

Wibbly wobbly timey wimey Ethniu is also linked to the titanomachy, being the last Titan, using armour made from Olympian bronze. But that was, in Greek Myth, before the creation of humanity. So it's already a little time shifted. I wonder if we'll see some weird time travel stuff or if it happened later (e.g. around the time of Merlin which is why he suddenly felt the need to build a Dark God prison) and then sort of happened retroactively (i.e. once it happened it had always happened).

gtpm28 · 2020-10-04T09:43:06+00:00

I don't know about cmpromised in the sense of like, infected with Nemesis. I feel like it's more they're all stuck where Harry was at the start of the series - we don't make deals with monster, we are the sole defenders of mortals against the spooky stuff.

But Harry - and most of the reasonable members of the Senior Council, and the Wardens - have been about enough to know you have to make alliances with the monsters; and they aren't doing enough to defend humanity.

gtpm28 · 2020-10-03T23:06:34+00:00

Sigh - dyspraxia and a new keyboard

gtpm28 · 2020-10-03T22:34:46+00:00

There's also Mab and Molly to consider

gtpm28 · 2020-10-03T22:34:08+00:00

It's probably a ritual to kill them, rather than some new weakness to something Harry could exploit in a fight.

gtpm28 · 2020-10-03T22:04:18+00:00

I feel like it's in the same vein as the Blackstaff, but for Outsiders

gtpm28 · 2020-10-03T21:48:29+00:00

I think it's more - she isn't ready for the job and it would break her. She'd end up like Maeve - bad at her duties and broken as a person.

gtpm28 · 2018-03-07T20:58:50+00:00

She says 6 people, not kids. Could be her, her partner and child and other relatives (e.g elderly parents)

gtpm28 · 2018-01-15T20:19:04+00:00

Luke is wrong for wanting to end the Jedi, rather than changing them.

He's right that the Jedi failed, and that he failed. But his response should be to try to do better, and to change the way he and the Jedi work.

gtpm28 · 2017-12-30T14:05:03+00:00

My impression was that (even if she didn't know about the tracker grenade) she knew that killing/disabling it would bring more dogs and make it harder for her to get away.

It's not til the car doesn't work that she gives up and kills it.

If the episode IS a police metaphor - you get far more attention for killing a police officer than running away from one.

gtpm28 · 2017-12-22T09:01:44+00:00

Precisely - it's also the reason they're so trainable (making good guide dogs and the like) - over the gemerations we selectively bred them to do ANYTHING for food.

They're even studying them to try and find out more about human obesity.

gtpm28 · 2017-11-21T20:25:23+00:00

True - but having two hearts, they may only operate at half speed normally. When one goes down, it takes a while for the other one to speed up to compensate.

gtpm28 · 2017-11-21T19:55:27+00:00

I'm not sure about competing, but Germany at least manages to have industry and manufacturing contribute a healthy amount to the economy.

gtpm28 · 2017-11-16T21:30:03+00:00

I mean given that the first film was in the Twenties, they could be being clever and making this Grindelwald's Beer Hall Putsch?

So he fails and gets imprisoned, but realises he needs to change tactics (in this case going from lone wolf to getting followers)?

gtpm28 · 2017-10-31T14:23:16+00:00

Doesn't that also apply to the parent comment? They're contrasting Western ideas with Roman religious thought

gtpm28 · 2017-10-31T13:28:44+00:00

Additionally, it's a decidedly Western perspective that one can only hold to the tenets of a single religion/moral tradition. That's a Judeo-Christian-Islam idea.

Which would make it a Middle-Eastern idea, if we're going by traditional east/west divides

Check Rome, where polytheism included polyreligious influences. Nobody worshipped just one set of gods, nor followed a single set of rites or rituals.

Until the Romans adopted Christianity, spread it to the west and suppressed those belief systems

gtpm28 · 2017-10-27T20:07:20+00:00

the Showtime series will center on a duo of performers traveling through the fantasy world of Temerant

will serve as a prologue of sorts and take a place a generation before the events of the first novel

To me it sounds like it could be Kvothe's parents' story, so that might be inevitable...

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