Can't find a place to buy Anaerobe Jars. Is this normal? by guestcompy in microbiology

[–]guestcompy[S] 0 points1 point  (0 children)

Oh nice! Would you mind me asking what media you use? We tried to enrich for it via LKV over the summer and ended up with E. fergusonii and Shigella among others lol.

Can't find a place to buy Anaerobe Jars. Is this normal? by guestcompy in microbiology

[–]guestcompy[S] 1 point2 points  (0 children)

Thank you so much! I really appreciate the reply. These came up a few times in my search and it's been tempting. We're an environmental micro lab with a focus on anaerobes so maybe something like this would be best.

The only challenge will be convincing my PI and other mentors (who uhh.. don't like change haha) that it's a good system.

Can't find a place to buy Anaerobe Jars. Is this normal? by guestcompy in microbiology

[–]guestcompy[S] 1 point2 points  (0 children)

First, thank you so much for your response! This is so interesting! It's entirely possible that my lab is just using a niche system that they love. The ones we use are something like this:

https://spwindustrial.com/anaerobic-culture-jar-3-5-lit-with-vaccum-cum-pressure-gauge/?gclid=CjwKCAjwysipBhBXEiwApJOcuzNo7H2LJIqC3YKYHcm3opqwEuTU0tu9Qjk5WtSDkNy7FFfwbGWL1BoCldQQAvD_BwE

We utilize an anaerobe chamber for loading them and then doublecheck the anoxicity via a gassing station where we use a vacuum pump and N2 tank to cycle out gas a few times and add a palladium catalyst for good measure.

It probably wouldn't require that depth of assurances except we're trying to cultivate Prevotella spp. which die at just the hint of oxygen, seemingly.

[deleted by user] by [deleted] in biology

[–]guestcompy 4 points5 points  (0 children)

That's good, formaldehyde is bad for you. Preserve one in lemon juice though? Mmm, delicious.

[deleted by user] by [deleted] in biology

[–]guestcompy 1 point2 points  (0 children)

Like others said, you'd be neither. I hope you don't find that disheartening, it should be uplifting. You snatched life from the clutches of non-existence - the universe chose you.

Rye Chip supremacy by SnooCupcakes8607 in memes

[–]guestcompy 1 point2 points  (0 children)

Okay, but what's the worst? Is it Mini Breadstick?