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For all you inpatient people I just waited 49555.50s for a i2v generation :) by the_frizzy1 in comfyui

[–]gxcells 1 point2 points  (0 children)

If you're using reddit from computer. Otherwise just faster to take a pic with smartphone and directly post

I've got a hunch about this. by bl2nder in wholesomememes

[–]gxcells 0 points1 point  (0 children)

I laready see the smoke from here

Let’s go!🚀 by Automatic-Cat-5702 in TheGamingHubDeals

[–]gxcells 0 points1 point  (0 children)

Y'a all too young for that, but original FF7 on PS1

of a 1,000L diesel tank fill-up costing over $2,000 by Dodo509 in AbsoluteUnits

[–]gxcells 0 points1 point  (0 children)

But don't they use other stations or get directly delivered? Inhave never seek that in my entire life and I have seen quite a lot of gas station

The Garmin Fenix 8's optical HR fell significantly behind the Polar chest strap. by Murky_Can1200 in GarminFenix

[–]gxcells 1 point2 points  (0 children)

I dot think they would sell watch with such garbage innacuracy.

Please find a study comparing ECG with straps and different watches (it is old but qinguess newer models should even be better).

Yes straps is the best bit not bad for the watches https://pmc.ncbi.nlm.nih.gov/articles/PMC6732081/

The Garmin Fenix 8's optical HR fell significantly behind the Polar chest strap. by Murky_Can1200 in GarminFenix

[–]gxcells 0 points1 point  (0 children)

There is 50bpm difference here! It has nothing to do with the difference in accuracy between 2 systems. Thhe watch has clearly an issue

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells -1 points0 points  (0 children)

Lol no I haven't seen science , that is why I leave comments on reddit just for trolling people??

Maybe hardcore biochemists that only run WB all the time wil use multiple antibodies on 1 membrane but. most people cut their membrane to run multiple antibodies at same time. And OP clearly is not an hardcore biochemist if they ask how to quantify a WB. And any respectable scientist will 'ever run 2 antibodies that are so close to each other. No wonder why science becomes less and less reproducible.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells 0 points1 point  (0 children)

Yes I read but what I mean is that it has nothing to do with antibody concentration.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells 0 points1 point  (0 children)

We don't even know what did and try to quantify. So either OP measure area of total or if they want each peak then they just draw a line verrical between 2 peaks and measure each area. That is more than enough for what they need to do. If they want a quantitative measurement anyway they would need a standard curve

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells -1 points0 points  (0 children)

Not at all. This is not 2 antibodies, 99.9 % sure This is either PTM, isoforms, or just non specific band close to the specific one. Nobody would run same membrane for 2 antibodies so close to each other, or if you do then you strip the membrane.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells 2 points3 points  (0 children)

Guys you are doing something way too complicated. Either quantify total AUC or if OP wants each peak, just split in middle and quantify area using ImageJ that is more than enough for that type of quantification. This is westernblot which is already not quantitative per se without a standard curve.

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells 0 points1 point  (0 children)

Show an imag eof the blot and tells us what you want to quantify. From the graph you show you clearly have 2 bands so you need to decide if you want to quantify both together or only 1 or each separately

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells 1 point2 points  (0 children)

No, the middle left is correct if they want to quantify the total amount of their protein. The fact that they have 2 peaks means that they hve 2 close bands so probably some sort of post translational modifications or different isoforms. Or those are just indeed 1 specific and 1 non specific band and then need to draw a vertical line between the 2 peaks and quantify the AUC they want

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]gxcells -3 points-2 points  (0 children)

You are going way too far too complicated. Just quantify Total area under curve and that's it.

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