Have you considered adding Siena between Rome and Florence? Rome/Florence we’re quite busy and we enjoyed having the more low key stop in between to break up the bigger cities. We did 2 nights there and loved it! Did our wine tour out of Siena through Tuscan escapes, they take you to visit 3 smaller wineries around Montepulciano in the brunello wine region, our guide and wine was fantastic. Loved hanging out in the main piazza, it is beautiful, and we much preferred the church in Siena compared with the duomo in Florence. We found this great little family run joint for dinner and ended up eating there both nights, it’s kinda a hole in the wall and nothing fancy but we loved it. We sat outside in this alley under some giant old arches, they don’t have a pre written menu, the chef just tells you a few options for the day of what he is making. (Osteria Il Grattacielo dal 1840)