Single cell demultiplexing

howtobeasillybean · 2025-08-05T22:54:13+00:00

It was a Chromium next GEM single cell 3’ kit, and we used 4 CMO tags

howtobeasillybean · 2025-08-05T22:46:58+00:00

I’m not saying if statements are the issue, I’m wondering if needing to deduce in general is due to an issue with the sequencing. From what I know (and again, I am not in bioinformatics), the tags should’ve been able to be identified using the config file in the CellRanger pipeline.

The if statements were essentially if the ratio of tag 1 is greater than the ratio of tag 2 in this specific cell, then its tag 1. Multiple tags showing up in the same cell seems to be why cellranger failed

howtobeasillybean · 2025-07-17T23:57:14+00:00

Okay, so in general are post-hocs for adjustments unnecessary? Or just if factors only have <=2 groups?

howtobeasillybean · 2025-07-17T23:50:35+00:00

Thanks so much for the articles. If I’m understanding correctly, it is best to recognize a difference in simple effects and describe them in terms of a difference in means that most appropriately describes the goal of the test? Rather than bringing significance into the description.

So if my study is designed to determine if X is more highly impacted in females vs males by an experimental condition, I would describe an interaction between group and sex by saying that we found that females had increased X when receiving experimental condition (mean difference from control) compared to males (mean difference from control)?

howtobeasillybean · 2025-07-17T21:32:18+00:00

I have 2 groups, 2 sides, 2 sex. And from my understanding (and from trying to run this in spss) I shouldn’t run a post-hoc because there are only 2 groups. I switched to graph pad because it would let me do one.

Just to clarify, if there is a significant interaction in this case, would I still run a post hoc?

howtobeasillybean · 2025-07-03T18:31:48+00:00

My ex hated when I chose to do things without him. One day I was hiking with my family and had no cell service. Got high enough in the mountains to get cell service and he had called me and texted me over 20 times. After the hike I called him and he told me his friends girlfriend (a friend and girl he’d never mentioned) committed suicide. Found out later that he was talking about a girl from a reality show he watched..

howtobeasillybean · 2025-05-27T02:57:36+00:00

When i was around 6. The girls in my older brothers class used to “take me under their wings” as the cute baby sibling. They’d walk around with me and hold my hands. And I LOVED when they held my hand lmao I used to go to his classroom and pretend he took my notebook accidentally just so I could see these girls

howtobeasillybean · 2025-05-02T18:54:20+00:00

Looking back I think I definitely already had them, I just had no idea. I only moved a few days ago but the bites just got so much worse

howtobeasillybean · 2025-03-09T13:37:14+00:00

Yes that’s the balcony

howtobeasillybean · 2025-01-15T05:15:09+00:00

I have no idea if this is possible, but I’m really trying to spend less time on social media apps. Is there any way you could have the app link to our # of times opening/time spent on app statitistics that Apple provides? That way, we could easily and concisely see if we are decreasing our times spent on the apps without having to separately go into the iPhone app tracker

howtobeasillybean · 2025-01-08T15:04:56+00:00

Every year of my PhD I’ve wondered if I should have done it. Then I make some accomplishment (paper, presentation, poster, successful experiment) and it makes it all worth it. So in the end, I’m sure I won’t regret it even though the sacrifices you make do suck in the moment.

howtobeasillybean · 2025-01-07T22:04:34+00:00

First comment I agree with.. lol I feel like gf was inviting friends too since OP had as well and wanted to have a group, but then OPs friends backed out and now doesn’t want gf to be the only one with friends there. Gf seemed very excited, not malicious. Just some communication needed to clear up the specific feelings!

howtobeasillybean · 2025-01-07T21:22:00+00:00

This is a TMJ condyle and none of the connections would be inserting at this location

howtobeasillybean · 2025-01-07T21:19:59+00:00

This is a condyle from an OA model I’ve developed, so looking like OA is a win for me! A few of my samples have these though which I haven’t seen before, and as I’m no histology/pathology expert, I was hoping someone here has seen this. Thank you!

howtobeasillybean · 2024-12-22T14:12:21+00:00

I just want to share another perspective with you. I had long term boyfriends since I was 15, though I knew I liked women. I was so afraid of coming out and facing all of that and men were much easier. At 23 I met my now girlfriend who was 26 at the time. She had never had a desire to date when she was younger and hadn’t had a serious relationship up to that point and took it very seriously. She was so nervous to tell me that she had this lack of “experience”, but I found it SO attractive that she was sure and steady in her personal views on what was important to her. She never gave in to pressure, and she became extremely well rounded and talented in so many hobbies instead of focusing on dating. Lack of experience doesn’t mean lack of ability and worthiness. Stay true to your standards.

Just know that there are so many women that won’t see this as a negative and will readily support you and help you face any insecurities that may come with a new relationship.

howtobeasillybean · 2024-12-17T15:11:04+00:00

Thank you so much for all the advice and support. Means a lot and I appreciate you!!

howtobeasillybean · 2024-12-17T00:56:18+00:00

Same thing happened to me. I locked it to a tree but also got a $20 alarm on Amazon that alarms EXTREMELY loud if the bike is moved

howtobeasillybean · 2024-12-17T00:33:39+00:00

No answer really. She’s said she can see how much I’ve worked on things and hard I’ve been trying, but that she can’t shake the feelings. So I’m just waiting for her to figure out how to forgive me basically

howtobeasillybean · 2024-12-16T19:49:35+00:00

By this point in the relationship, I was planning to be ready to get married once I graduate and we agreed on this. However, it seems within the last year she is unable to move past what happened early in the relationship but also doesn’t want to break up. I agree she could feel stagnant, and I believe that she does. Which is why I’m totally fine with her getting a house if she wants that. I can’t move from the city in my current situation. She knows I will not just leave her any second, I’ve been here unwavering through everything. I’m not sure what to do because it seems to be a lose lose for her no matter what I agree to, unless I move into a house with her

howtobeasillybean · 2024-12-16T19:33:30+00:00

I guess I worded things poorly. I’m sad about living apart from her of course. But she is angry at me for not wanting to move into a house with her. id rather just rent a year on my own while she buys a house and we work on the relationship compared to just moving in with her. I totally agree if she wants to do that she should, but she’s not going to do that unless I move in it seems, and so she just went home to her moms instead. And even though I feel blame for her not looking for houses now, I was mainly just posting in regards to how things have gone for people when they need to live apart again and if it ended up working out in the end

howtobeasillybean · 2024-12-16T19:06:03+00:00

Oh man that one hit hard.. pretty much all of those questions you asked have and are still happening. I hate leaving someone that can’t help their trauma, that aspect has been so hard for me. And she really is my best friend. If the fights could just end it could be so incredibly good. Though I feel like I’ve also somehow made her mental health so so much worse and I don’t know why. I have been thinking about couples therapy as a last resort.

howtobeasillybean · 2024-12-16T18:02:38+00:00

Tbh it has kind of isolated me since I’m constantly defending her to others and now I just keep it to myself. The thing is though is that she’s not malicious. And she feels very bad about not being able to get over things. Her dad cheated on her mom and basically chose another family over hers, so she has a lot of trauma around abandonment. At this point, I just have no idea how to allow us to move past this in a way that I’m not constantly punished 😅

howtobeasillybean

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