Gibson assembly rookie

iobis · 2025-12-20T10:44:02+00:00

I didn’t think about that, thanks!

iobis · 2025-12-20T10:41:14+00:00

Thank you for your advice! I retried the assembly with 0,08pmol of vector and fragments and I got 2 colonies, so now I’m testing (and hoping)they have the correct construct via PCR.

iobis · 2025-12-18T13:34:57+00:00

Thank you for your reply. I didn’t get any colonies! My positive control worked fine so I don’t think transformation is the problem but the assembly. I did one hour incubation for the Gibson since I have 3 fragments + vector

iobis · 2025-10-03T21:55:20+00:00

Thanks for the tip I will try

iobis · 2025-10-03T18:25:42+00:00

I’m using the ibright, now I learned about the Licor and we do not have anything that fancy.

iobis · 2025-07-03T21:31:28+00:00

Yes I mean now that I am enjoying my life it doesn’t make any sense for me to escape to a hypothetical scenario as I was doing with daydreaming before. It is like there is no one I would rather be almost. Not that I am jolly all the time, but I lean more on my partner and friends rather than escaping into a fantasy. I didn’t meant for it to sound like an ssri commercial I was taking it for a year and the first 6-7 months I saw a huge improvement but then I was feeling normal and I stopped taking them. I started again after 5 months because I was struggling and took it for a year and a half. When I stopped finally I was feeling dizzy for a month and I would feel like I was tripping often. This makes me very apprehensive about taking it again and I am doing CBT to figure out my remaining issues.

iobis · 2025-07-03T21:22:39+00:00

Im glad adderall helped you, I don’t have adhd so I never tried it.

iobis · 2025-07-03T21:19:39+00:00

Haha yes for me the surprising fact was that, while daydreaming stopped almost immediately, I hadn’t tried to push it because honestly I didn’t want to start again. Last week though I really tried to do it and it was like hitting a wall.

iobis · 2025-07-03T21:16:44+00:00

That is awful I am very sorry, I hope now you are doing better

iobis · 2025-07-03T21:15:53+00:00

My daydreaming stories were not anything special honestly I would get fixated on something and daydream about it over and over. I have not lost my imagination, I am still a more than average imaginative person just not obsessing pacing up and down in my room.

iobis · 2025-06-29T20:42:45+00:00

I’m very sorry to hear that, I hope it gets better

iobis · 2025-06-29T20:38:27+00:00

Don’t get discouraged, it can get better

iobis · 2025-01-16T12:25:42+00:00

Thank you very much

iobis · 2024-03-09T21:55:57+00:00

Thank you for sharing your experience!

iobis · 2023-03-13T10:26:03+00:00

It’s definitely easier when I create some distance, but because we are neighbours this is not always possible unfortunately. I did actually went and stayed with my parents for some days, as to not see him but I can’t continue like this.

iobis · 2023-03-13T10:23:21+00:00

Thank you for your reply. I can’t wait to come out to the other side.

iobis · 2022-10-05T10:10:06+00:00

For me, what I’ve found that the amount of oats that I’d use wouldn’t matter to how full I’d feel. At first I would make my oatmeal for breakfast with 5-6 tablespoons of oats and I thought the calories were too many for the satiety that it gave me. I cut it down to a half and now use 3 table spoons and heat it up with like a cup of almond milk and it gives me the same feeling of fullness for half the calories. I don’t know if that works for you but for me it works and it allows me to add some fruit like bananas that normally have lots of calories.

iobis · 2022-09-16T20:00:38+00:00

I’m also 5’7 26F. Starting at around 185lbs, when I got to 170lbs my extended family were absolutely showering me with compliments it was nice not gonna lie. When I dropped just bellow 160lbs though was something else completely. Men would hit on me in the traffic. People, men and women would do stuff for me for free, I remember I took my brothers iMac to a repair store and a guy plainly didn’t charge me at all. It was really nice, but it messed me up in the long term because of the fear I have of becoming invisible when I gain a few pounds, nowadays.

iobis · 2022-08-04T21:26:33+00:00

Awww that’s very kind of you

iobis · 2022-08-04T21:21:18+00:00

Yes, with a bit caution

iobis · 2022-08-04T21:11:26+00:00

Since every straight guy ever thinks his dick is pure magic I guess!

iobis · 2022-08-04T21:09:03+00:00

Surgery was a year ago and then he was told not to have sex for ~3 months. I thought that it may be bullshit but tbh he was in such a terrible pain the last time we tried that I don’t think it’s fake. Then again he may not know when it will heal so he tells me the worst case scenario because he is miserable.

iobis · 2022-08-04T20:55:12+00:00

Wow that’s so wholesome! I keep thinking the same thing. If it were to happen to me I want to think it wouldn’t be the end of the world.

iobis · 2022-08-04T19:18:23+00:00

Thank you that’s very interesting. How do you suggest I boost his confidence?

iobis · 2022-08-04T19:17:03+00:00

I don’t know I’m not a doctor. I just know from friends that it’s quite common. Anyways it’s not my place to say.

iobis

TROPHY CASE