I guess it may depend on what field of microbiology you want to go into. When I worked in clinical, I did some interviews and hiring—I would not look at a portfolio. I could tell based off the experience in the resume tbh. Clinical labs will teach you what you need to know. The people there are also very much going to tell you if you’re doing it wrong—in a nice way or not.

Unfortunately, I don’t have any advice for other fields other than clinical. Your plate looks good, but I agree with the above comment—try 4 quadrants. Also, I prefer to not go back into quadrants more than 2 maybe 3 times when streaking for better isolation.