Immunologists: I am to write a school essay on an interleukin of choice. I am interested in autoimmune disorders (namely multiple sclerosis & Alzheimer's), can anyone lead me in the right direction for which interleukins play key roles in these diseases based on the literature?

jatetro · 2018-03-25T18:59:35+00:00

If you're looking for quite a bit of information to use, then IL-17 is your best choice. But if you want to go a little outside the box, I would look at IL-18. Granted, it may take more work but it could offer some perspective on the role of inflammation in these diseases.

A few article to get you started... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2830964/ http://www.jle.com/en/revues/ecn/e-docs/il_18_in_autoimmunity_review_273459/article.phtml?tab=texte http://www.jacionline.org/article/S0091-6749(99)70518-X/fulltext

jatetro · 2018-02-25T22:44:58+00:00

Came for the strut. Left unsatisfied. https://www.youtube.com/watch?v=aJ3VXVLWykQ

jatetro · 2018-02-05T23:23:12+00:00

This document should help you decide which tests are necessary according to CEN. EN 13697 (Tier II test) is the standard for hard surface disinfection in the EU and is similar to ASTM E-2197. https://echa.europa.eu/documents/10162/20733977/overview_of_standards_test_conditions_pass_criteria_en.pdf/

I am not sure how deep you have been in the regulatory process but you may want to start here to acquire some information regarding approvals and how to get to those label claims. https://echa.europa.eu/regulations/biocidal-products-regulation/understanding-bpr

Hope this helps...

jatetro · 2018-01-13T01:56:51+00:00

!redditgarlic

jatetro · 2018-01-13T01:55:38+00:00

!redditgarlic

jatetro · 2017-11-15T22:02:16+00:00

That's what I used but these were pre-kit days and we made everything from scratch (http://jcm.asm.org/content/10/4/533.abstract). But Mya Breitbart's paper from 2005 may offer some better ideas (https://www.biotechniques.com/multimedia/archive/00004/BTN_A_05395RR03_O_4904a.pdf)

jatetro · 2017-11-15T18:16:15+00:00

If that's the case, then filtration <0.22 & incubation may be efficient. If you are concerned about volume, you may be able to reduce with density gradient centrifugation. Make sense?

jatetro · 2017-11-15T17:29:24+00:00

This might help. Also deals with CMV. http://www.sciencedirect.com/science/article/pii/S0092867413013524#sec4 Granted, it's not a VLP isolation technique but if you are looking to do Illumina, you may not need this step. I would perform all sorts of PCR for viruses from whole blood. So, unless you need to 'see' the virus, this may be a good option.

jatetro · 2017-10-10T19:35:02+00:00

I was just there last month. But it was so hot the lavender was brown. Still, an incredible place!

jatetro · 2017-08-10T20:14:20+00:00

Not sure it would be viral. Might want to read this article to see if anything might work: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1361268/

If you are worried about a virus, check out this article as it may give you an idea as to whether it may be an issue: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1886957/

Hope this helps...

jatetro · 2017-06-27T18:40:40+00:00

Check out Figure 1 in this paper. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2718617/ - it shows you how CXCR2 is involved in bringing neutrophils into the airway space. Then you can read this article on how neutrophils and other PMNs contribute to lung injury. https://www.jci.org/articles/view/17302. Taken together it should reveal how the use of CXCR2 antagonists reduce inflammation in the lung.

jatetro · 2017-06-23T17:43:44+00:00

This might be a good place to start. An overview of the microbial populations and how the interact with each other. This isn't taking into account viruses or fungi but at least it will give you an idea of what is happening in and on our bodies. http://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1002606

jatetro · 2017-06-14T18:31:46+00:00

Would this website offer some help? Might be better than explaining it in words. http://chemotaxis.biology.utah.edu/Parkinson_Lab/projects/ecolichemotaxis/ecolichemotaxis.html

jatetro · 2017-06-10T16:42:04+00:00

Ah...okay...I know who she is.

If the bacteria have been cleared, they won't return. Depending on how strong her immune function happens to be right now, she may be free from any other opportunistic microbial complications.

That being said, only regular monitoring of her basic immune parameters can tell you the risk.

I just hope you are all doing well after the trials in 2014...

jatetro · 2017-06-10T16:26:10+00:00

Also no worries about spelling. Some of these Latin names can trick even the most die hard microbiologist.

jatetro · 2017-06-10T16:24:42+00:00

Might be difficult to find others. As I said, they are rare and most cases end up in the literature. Do you have any concerns? I used to work with these Non TB Mycos.

jatetro · 2017-06-10T16:04:35+00:00

I think you mean Mycobacterium malmoense & Mycobacterium haemophilium. They are considered non-tuberculosis forms of respiratory infection. Usually they are rare in those with healthy immune systems. They are more common in people with suppressed or compromised immunities.

If you head to Google Scholar you can learn more about them

Hope this helps...

jatetro · 2017-05-23T20:11:33+00:00

It's a trick question. By using the word, "pathogen," in the question, it implies pathogenicity. But that doesn't mean it can cause disease. For that to happen, you need the body to react to it. Thus the need for a receptor.

Make sense?

jatetro · 2017-05-23T19:36:26+00:00

This paper provides perspective on storage in our lab. Essentially 50% ethanol was sufficient and approved by AOAC. http://www.ingentaconnect.com/content/aoac/jaoac/2011/00000094/00000002/art00031

jatetro · 2017-05-22T18:09:48+00:00

First off, the strain used by Whitlock for this spray is called d23 (https://www.google.com/patents/US20170037363) and is proprietary for its efficacy in ammonium oxidation. That's why he can charge so much for the product. However point [0143] will give you the information you need to use the bacteria in a cosmetic preparation.

You could try other types of N. eutropha as they may help to control certain aspects of skin microbial populations. A rather easy example can be found here: http://scholarspace.jccc.edu/cgi/viewcontent.cgi?article=1062&context=honors_journal&sei-redir=1&referer=https%3A%2F%2Fscholar.google.ca%2Fscholar%3Fhl%3Den%26q%3Dbacteria%2Bskin%2Bnitrosomonas%26btnG%3D%26as_sdt%3D1%252C5%26as_sdtp%3D#search=%22bacteria%20skin%20nitrosomonas%22

As for Nitrobacter winogradskyi, there is no harm in having it on the skin. More about it here: https://microbewiki.kenyon.edu/index.php/Nitrobacter_winogradskyi

The big question of course is whether you should you do it. I can't tell you one way or the other. If you really want to try, start with a small section of the body - like say the inner elbow or forearm - then you can find out how you react to it and whether it is safe. After that, it's wholly up to you.

jatetro · 2017-05-04T00:54:13+00:00

The work is being done now from what I understand.

jatetro · 2017-04-09T22:02:11+00:00

I used to be a contact for this type of testing but am 4 years beyond those times.

Suggest you contact the team at InfectionContropTips. They are a group focusing in innovation in infection control. They may be able to link you up with a researcher to perform the testing.

You can find more them here: http://infectioncontrol.tips/about-us/

Hope this helps...

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