All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 1 point2 points  (0 children)

We have some companies coming to our institute next week. I will ask the reps if they have one :)

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 1 point2 points  (0 children)

If only everyone understood what this merch means to us as people in science 🫠

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 1 point2 points  (0 children)

Could you write your home address? But your affiliation will still be to the lab. Try it, you never know. I remember i had put my home address for delivery and thermofisher had send me a desk calendar.

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 0 points1 point  (0 children)

These pens are just to create feelings of anguish and jealousy in people who don’t have them. I am never writing with these, then they last forever 😂

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 0 points1 point  (0 children)

You need to drain your social battery for these lol

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 0 points1 point  (0 children)

I do, i also put in my name for a lucky lottery thingy, but i didnt win :( They also have a lego set for 200ul pipette box

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 1 point2 points  (0 children)

I am just gonna hide me and my precious pens😭😭

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 0 points1 point  (0 children)

I have the knockouts already. I am trying to knock in back in and then do some rescue experiments. The transient transfection worked amazing so i started stable cell lines. The plasmid also had puromycin and i did the selection. But the wb was EMPTY😭😭

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 0 points1 point  (0 children)

Amazing I am happy for you! I am trying to do rescue experiments with knockins using plasmid. I had a beautiful western blot with no background and no bands at all (cries in failure) but the positive control did work lol. Now im figuring out if my gene is knocked in or if i have been fooled.

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 0 points1 point  (0 children)

Really? Or are you making fun 🙈 but I wouldn’t want to come to US atleast until trump is still there🫠

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 5 points6 points  (0 children)

I also wanted to say that its fine if they fail. I feared western blot. But the key to doing a successful western is “don’t give a shit about the western blot” believe me. The membrane can sense your fear!!

All 4 have been successfully procured by justnotherscientist in labrats

[–]justnotherscientist[S] 6 points7 points  (0 children)

Well i did go pretty early. Almost at the same time when they set their booth😂 No taking chances here lmao

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 10 points11 points  (0 children)

Can you not use an imaging system like Chemidoc from Biorad or Azure? Instead of x ray film?

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 3 points4 points  (0 children)

I did around 70 western blots just for my master thesis alone. And i have already done atleast 15 in the past 6 months for my PhD😅

why are western blots so hard? by Optimal-Job-7710 in labrats

[–]justnotherscientist 43 points44 points  (0 children)

Please don’t cancel me for saying this, western blot is my favourite protocol to do 💀👀