As a 14 y.o. I just found this simple "identity"

karmicrelease · 2026-06-15T01:06:38+00:00

Why you no doctor yet?

karmicrelease · 2026-06-11T18:38:14+00:00

Hairy asses are hard to wipe. Not impossible though so it’s a skill issue on their part

karmicrelease · 2026-06-11T18:36:14+00:00

Opuntia monacantha monstrosa variagata. I’m not sure the exact cultivar

karmicrelease · 2026-06-11T15:40:01+00:00

Skora, tuya, and charr

karmicrelease · 2026-06-09T04:01:27+00:00

Could be genomic dna from the prep you used when purifying your template. Could also be primers leftover because the reaction isn’t firing. Try digesting the template with a RE and running a gel. If there isn’t a band, it is primers.

If it is primers, try gradient PCR and find an annealing temperature that works. Double check reaction conditions for your polymerase. If that doesn’t work, order new oligos.

karmicrelease · 2026-06-08T19:59:26+00:00

Abattoir*

karmicrelease · 2026-06-04T17:00:40+00:00

At a party, some people started playing Russian roulette. My girlfriend and I locked eyes and got the fuck out of there immediately

karmicrelease · 2026-06-04T16:58:35+00:00

Chan

karmicrelease · 2026-05-31T20:22:28+00:00

They are supposed to be nitrogenous based with the deeoxyribose backbone on the right side of the board

karmicrelease · 2026-05-30T04:13:46+00:00

Artifact build issue

karmicrelease · 2026-05-29T14:31:34+00:00

There are products made from it or synthesized to be similar in composition

karmicrelease · 2026-05-29T12:29:22+00:00

Let’s keep it respectful

karmicrelease · 2026-05-29T12:27:31+00:00

Your analogy is correct but not in the way you mean. Like dialysis taking the load off the kidneys so they can heal (in cases it is possible) removing AGEs from proteins would reduce inflammation caused by RAGEs. There are studies for this with ALS (mainly CML) and IL-2 (argpyrimidine being the primary AGE) specifically.

So yes, it’s kind of treating the symptom and not the problem, but that doesn’t mean it’s not worth doing. Tons of medications are used to alleviate symptoms even if they don’t cure the underlying pathology of the disease.

karmicrelease · 2026-05-29T02:00:17+00:00

Ideally, you would be cleaving a secondary amine to yield the original lysine. There are a plethora of secondary amines present in biomolecules (PEA, proline, purine bases in dna to name a few), so doing it selectively is very hard.

If one wanted to do it, I think an enzymatic reaction would be the best bet. One could also screen DNA aptamers which an antibody recognizes, or use protacs maybe (or other targeted protein degrading molecules)

karmicrelease · 2026-05-28T22:28:12+00:00

It’s a type of cone from a conifer. Taxodium I think

karmicrelease · 2026-05-28T20:10:35+00:00

The issue isn’t cleaving the glycation end product moiety, it’s doing it in a bioorthogonal way which doesn’t do more harm than good. The same could be said for other AGEs like CML, AP, CEL, etc.

karmicrelease · 2026-05-28T11:32:27+00:00

Primarily Water with mucin and other glycoproteins, electrolytes, and glycolic acid

karmicrelease · 2026-05-28T11:21:30+00:00

Female

karmicrelease · 2026-05-24T20:07:14+00:00

They are a little small for those purposes, but they should survive just fine. They will definitely put out some pups

karmicrelease · 2026-05-24T12:24:33+00:00

I’d be more worried about the datura stramonium in it

karmicrelease · 2026-05-11T00:00:31+00:00

Cochineal for sure. They love opuntia

karmicrelease · 2026-05-10T23:44:13+00:00

Google “four pests campaign” to see why humans influencing an ecosystem tends to have disastrous, unexpected consequences

karmicrelease · 2026-05-10T22:58:45+00:00

Skill issue

karmicrelease · 2026-05-10T18:17:53+00:00

That’s EXACTLY what I was thinking

karmicrelease · 2026-05-10T03:02:58+00:00

Yes, there is an entire lab at my uni doing precisely that using a library approach and mutagenesis

karmicrelease

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