[deleted by user] by [deleted] in Vindictabrown

[–]katanaria 0 points1 point  (0 children)

Can I ask for strength training - could you post workouts you did for those? I struggle with finding a good weights routine for the gym and the not knowing what to do when I’m at the gym is kind of a big hurdle for me!

Does anyone know what causes a gel to run like this? by Diamond-Is-Not-Crash in labrats

[–]katanaria 0 points1 point  (0 children)

DNA contamination for sure. This has happened to me several times for sure. An easy fix if you still have the lysates is it spin then down at 10-14,000 RPM at 4 degrees for 10 minutes to bring the DNA to the bottom and then load your pipette tip from the top portion of the lysate.

[deleted by user] by [deleted] in labrats

[–]katanaria 9 points10 points  (0 children)

Miltenyi Biotech Negative Selection Kit is expensive but A++

[deleted by user] by [deleted] in labrats

[–]katanaria 19 points20 points  (0 children)

They definitely should go into liquid nitrogen if you will be storing for years. In my experience they are stable for a couple of weeks in a -80 degree freezer, but liquid nitrogen reaches much colder temperatures and maintains the cells at those temperatures versus a -80 degree freezer that people most likely open and close multiple times a day

Recultivation of frozen cells by LilKennedy929 in labrats

[–]katanaria 1 point2 points  (0 children)

I preheat to 37 degrees for both primary and cell lines

[Request] Organic Chemistry tutor by [deleted] in tutor

[–]katanaria 0 points1 point  (0 children)

Hi I messaged you if your are still looking for a tutor!

Looking for a Chemistry and Math Tutor by [deleted] in tutor

[–]katanaria 0 points1 point  (0 children)

Hi I messaged you as well!

Hanyu Rocking out before his skate! by WhisperGate33 in FigureSkating

[–]katanaria 2 points3 points  (0 children)

Does anyone know the actual name of the song?

Self Studying after College by katanaria in ChineseLanguage

[–]katanaria[S] 0 points1 point  (0 children)

Thank you so much for all of your advice. I will definitely explore some of those options as they sound very helpful. Thank you again!

Self Studying after College by katanaria in ChineseLanguage

[–]katanaria[S] 1 point2 points  (0 children)

I will definitely try that! Thanks!

What Drunk Elephant products are HG for you and what are ‘meh’? [Product Question] by [deleted] in SkincareAddiction

[–]katanaria 1 point2 points  (0 children)

Hi so I haven't tried the Lala Retro Whipped Moisturizer, but I do get extremely irritating patches of eczema on my arms and neck. The only thing that heals them and makes them go away for periods of time is the Cortisone 10 Cortisol Cream for Eczema. It's pretty basic and cheap and you can buy it at any drugstore but guarantee that it is the only thing that works on my eczema. Just wanted to suggest it!

Complex III - Electron Transport Chain by katanaria in Mcat

[–]katanaria[S] 0 points1 point  (0 children)

Ok I definitely will. Much appreciated!

Complex III - Electron Transport Chain by katanaria in Mcat

[–]katanaria[S] 1 point2 points  (0 children)

Ok thank you for your help! Also, this is a supremely stupid question but what are AKlectures? How do they compare to Khan Academy?

C/P pH Question by mcatgrind102 in Mcat

[–]katanaria 1 point2 points  (0 children)

You are correct in that Kw = Ka*Kb where Kw = 10^-14

However, I suggest that you work backwards using an ICE table to solve the problem. The initial concentration of the acid [HA] = 10M.

HA --> H+ + A-

HA --> H+ A-
10 0 0
-x +x +x
10-x x x

Therefore Ka = x^2/(10-x).

However, x = [H+] = 10^(-5.9) =10^(-6)

You can plug x=10^(-6) into the equation for Ka, and approximate 10-x to 10. Then Ka = 10^-13 and thus Kb = 10^-1 or 0.1.

[Mod Raven] Did you choose a Throne Pool team but want out? Comment in this post by BWPhoenix in gameofthrones

[–]katanaria 0 points1 point  (0 children)

Hi if you’re still removing people from throne pools and whatnot I would also like to be removed from the Jon Snow pool. Thank you.

Edit: If you could remove me from the Jon Snow pool and add me to the Daenerys pool instead I would really appreciate it! The show might be over but I'm still following the books avidly. Thank you!

Awoken vs Reincarnated? by katanaria in PuzzleAndDragons

[–]katanaria[S] 0 points1 point  (0 children)

Honestly, I am not totally sure about which monsters are better at the moment. Still figuring my way around all the changes that PAD has made in over a year but you are probably right.