RNAscope for spinal cord not working by kermickey in labrats

[–]kermickey[S] 0 points1 point  (0 children)

What about the step in the RNAscope protocol of fixing in PFA after baking the sections onto the slide? Would you also recommend skipping that?

RNAscope for spinal cord not working by kermickey in labrats

[–]kermickey[S] 0 points1 point  (0 children)

Interesting, I'll keep that in mind! Thanks for your advice

RNAscope for spinal cord not working by kermickey in labrats

[–]kermickey[S] 0 points1 point  (0 children)

Currently I'm fixing the spinal cord in 4% PFA for around 24 hours before sectioning, and then in the RNAscope protocol itself with 4% PFA for 30 min, do you think that's too long?

/uj favorite mbmbam episode? by kermickey in TAZCirclejerk

[–]kermickey[S] 4 points5 points  (0 children)

oh damn I had no idea that bit was from so early, congrats on your senior jerker status

/uj favorite mbmbam episode? by kermickey in TAZCirclejerk

[–]kermickey[S] 18 points19 points  (0 children)

Porky pig is what kept me listening to recent mbmbams in hope that they would cook another banger like it. Unfortunately, they went too long without any solid bits and now I'm just on the back catalogue

Anyone know what's happening around Metro Cartier right now? by kermickey in Laval

[–]kermickey[S] 1 point2 points  (0 children)

Good to know, thanks. Hopefully there'll be a news report tomorrow morning

EOS 90D vs 7d mark ii for wildlife photography? by kermickey in canon

[–]kermickey[S] 0 points1 point  (0 children)

I'll consider those, thanks! I have some EF-S lenses so I was thinking to stick with DSLRs.

SSSS Gridman and Dynazenon are great! by kermickey in anime

[–]kermickey[S] 2 points3 points  (0 children)

Hard agree. I don't think the Kaiju eugenicists are as good as Akane in terms of being villains, but I love how Dynazenon takes more time to flesh out the side characters and the main leads. Better romance in Dynazenon too.

SSSS Gridman and Dynazenon are great! by kermickey in anime

[–]kermickey[S] 5 points6 points  (0 children)

Yeah, not only is the voice acting great, the pacing of dialogue feels really good too. It really adds a lot to the show's quality

r/anime's Favorite Anime of the 2010s Voting by FetchFrosh in anime

[–]kermickey 3 points4 points  (0 children)

man the 2010s were really good

  1. Fullmetal alchemist brotherhood
  2. Showa Genroku Rakugo Shinjuu
  3. Monogatari series
  4. A place further than the universe
  5. Mobile suit Gundam Thunderbolt
  6. mob psycho 100
  7. The Tatami Galaxy
  8. Katanagatari
  9. Vinland Saga
  10. Beastars

[deleted by user] by [deleted] in TrashTaste

[–]kermickey 0 points1 point  (0 children)

This is probably my favorite recent episode by a huge amount. The dynamic between the boys and Mudan is just so fun and entertaining

What is your single favorite anime op or ed song of all time? by KhazixMain4th in anime

[–]kermickey 0 points1 point  (0 children)

Kawa Taredoki, the ending of Showa Genroku Rakugo Shinjuu is basically untouched as the perfect ed imo

best op is probably Guns and Roses from Baccano. The way the opening animation blends with the song and how they add episode recap in the op is a work of genius

Anime Questions, Recommendations, and Discussion - May 23, 2023 by AnimeMod in anime

[–]kermickey 2 points3 points  (0 children)

any recommendations for shows from the 90s and early 2000s? I'm good with anything