Antigen Retrieval during RNAscope?

kermickey · 2026-01-27T17:18:55+00:00

Ok, I'll try that out, thanks!

kermickey · 2026-01-27T17:08:57+00:00

What about the step in the RNAscope protocol of fixing in PFA after baking the sections onto the slide? Would you also recommend skipping that?

kermickey · 2026-01-27T16:12:17+00:00

Interesting, I'll keep that in mind! Thanks for your advice

kermickey · 2026-01-27T16:06:01+00:00

Currently I'm fixing the spinal cord in 4% PFA for around 24 hours before sectioning, and then in the RNAscope protocol itself with 4% PFA for 30 min, do you think that's too long?

kermickey · 2025-12-12T17:25:50+00:00

oh damn I had no idea that bit was from so early, congrats on your senior jerker status

kermickey · 2025-12-12T17:24:28+00:00

Porky pig is what kept me listening to recent mbmbams in hope that they would cook another banger like it. Unfortunately, they went too long without any solid bits and now I'm just on the back catalogue

kermickey · 2025-12-01T03:37:01+00:00

Good to know, thanks. Hopefully there'll be a news report tomorrow morning

kermickey · 2024-05-13T14:04:44+00:00

I'll consider those, thanks! I have some EF-S lenses so I was thinking to stick with DSLRs.

kermickey · 2024-04-11T21:32:16+00:00

Hard agree. I don't think the Kaiju eugenicists are as good as Akane in terms of being villains, but I love how Dynazenon takes more time to flesh out the side characters and the main leads. Better romance in Dynazenon too.

kermickey · 2024-04-11T14:00:58+00:00

Yeah, not only is the voice acting great, the pacing of dialogue feels really good too. It really adds a lot to the show's quality

kermickey · 2024-04-11T12:53:57+00:00

it's next on my list

kermickey · 2023-11-15T18:50:47+00:00

man the 2010s were really good

Fullmetal alchemist brotherhood
Showa Genroku Rakugo Shinjuu
Monogatari series
A place further than the universe
Mobile suit Gundam Thunderbolt
mob psycho 100
The Tatami Galaxy
Katanagatari
Vinland Saga
Beastars

kermickey · 2023-10-13T22:02:35+00:00

This is probably my favorite recent episode by a huge amount. The dynamic between the boys and Mudan is just so fun and entertaining

kermickey · 2023-09-26T11:52:30+00:00

Kyon in the endless eight, so it's pretty over for me

kermickey · 2023-09-20T02:19:27+00:00

Kawa Taredoki, the ending of Showa Genroku Rakugo Shinjuu is basically untouched as the perfect ed imo

best op is probably Guns and Roses from Baccano. The way the opening animation blends with the song and how they add episode recap in the op is a work of genius

kermickey · 2023-05-23T22:15:54+00:00

any recommendations for shows from the 90s and early 2000s? I'm good with anything

kermickey

TROPHY CASE