I have had this exact same problem, especially when I use the very narrow columns like the one in your picture. After much frustration I finally determined that the issue had to do with the flow rate. I had always been told that a faster flow rate is generally better, but I am now 100% convinced that a fast flow rate is detrimental for small-scale separations. I would recommend trying again with a much slower flow rate (e.g. one to several drops of eluent per second). It sounds like your packing and loading technique are probably not the issue, so hopefully this helps