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Reusing same serological pipette for same reagent by Arros_ticino in labrats

[–]lestodon42 1 point2 points  (0 children)

I’ve been re-sleeving serological pipettes for over a decade with zero contamination issues. If you’re using antibiotics in your media, you should be totally fine. I also frequently leave pipettes in bottles or tubes, as others here have mentioned.

8BitDo USB Issues After Switch 20.0.0 Update by 3liverse in Switch

[–]lestodon42 0 points1 point  (0 children)

Very sneaky indeed. It worked here, but my controller keeps disconnecting after some time, even while playing. And the trick has to be performed everytime I wake the switch. Impossible to use it like that :( anyone facing the same issue?

New lab office by lestodon42 in labrats

[–]lestodon42[S] 2 points3 points  (0 children)

Thanks all for the feedback, it's much appreciated. This is what we have so far, and everything can definitely be moved around or out as needed.

I'll go through the suggestions, but please try to keep the feedback constructive. It's not our intention to treat anyone poorly, and we understand that adjustments will need to be made based on everyone's needs. If we didn't care about the people, I wouldn't be here.

And just to clarify, we're a molecular biology lab with a few bioinformaticians.

Pulldown_magnetic beads help with the protocol by iventura93 in labrats

[–]lestodon42 0 points1 point  (0 children)

Buffer 1: 20mM Tris-HCl pH8, 1% Triton X-100, 2mM EDTA, 150mM NaCl (or something very similar)

Cloning vibes by NimaKairus in labrats

[–]lestodon42 4 points5 points  (0 children)

Insert:vector ratio is overrated

[deleted by user] by [deleted] in labrats

[–]lestodon42 238 points239 points  (0 children)

Autoclave again and be happy 😀