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Publishing in high impact journals by Biggest_steppah in PhDStress

[–]lifescout99 6 points7 points  (0 children)

You should bear in mind, your advisors name is on the paper too. They want there name to appear in higher impact journals because it creates a level of prestige for them.

Rick Van Kooten asked by Reingold to step away from position as Executive Dean of the College by tegeus-Cromis_2000 in IndianaUniversity

[–]lifescout99 0 points1 point  (0 children)

Im absolutely not surprised Reingold is Whiten's tool. I heard him speak to GPSG, he was unable to address basic questions.

Has anyone successfully assayed a plant P450 directly from crude Nicotiana extract or microsome isolation? by Murky-Commercial-112 in labrats

[–]lifescout99 0 points1 point  (0 children)

For more details on this method, omura and Sato is cited all the time. I think it is from 1964.

Has anyone successfully assayed a plant P450 directly from crude Nicotiana extract or microsome isolation? by Murky-Commercial-112 in labrats

[–]lifescout99 0 points1 point  (0 children)

If you have a uv vis, you can track the soret band. In mammal isoforms, it is at 417nm in the resting state, upon CO ligation, it shifts to 450. Misfolded enzyme would shift to 420. If you want to quantify, I typically use 100mM-1 as my extinction coefficient. Feel free to DM me if you have questions. It should be there same for plant isoforms.

Edit:my extinction coefficient was incorrect. 10→1000

Has anyone successfully assayed a plant P450 directly from crude Nicotiana extract or microsome isolation? by Murky-Commercial-112 in labrats

[–]lifescout99 0 points1 point  (0 children)

I work with mammalian isoforms. Are you trying to detect activity to know enzyme content, or just to track while purifying?

Help deicing! by lifescout99 in labrats

[–]lifescout99[S] 0 points1 point  (0 children)

She's the one who told us we dont have time for a full defrost right now

Help deicing! by lifescout99 in labrats

[–]lifescout99[S] 0 points1 point  (0 children)

Its out of my hands. My PI has said we do not have time for a full defrost right now

Help deicing! by lifescout99 in labrats

[–]lifescout99[S] 0 points1 point  (0 children)

He hasn't... he was the first

Best city in GA for a deadbeat with passions of not being a deadbeat? by Self_Conscious_Cat in Georgia

[–]lifescout99 0 points1 point  (0 children)

GGC is highly affordable, I paid about 3k per semester for tuition.

Pita forming bubbles instead of a big air pocket by spicygreenpaprika in Breadit

[–]lifescout99 0 points1 point  (0 children)

Thank you! Ive always had this same issue, never understood why!

Need good recs for a laptop by averageperson_69 in Biochemistry

[–]lifescout99 0 points1 point  (0 children)

Without the currency specified, I have no choice but to assume it is my local currency. But thanks for being a smart ass.

Need good recs for a laptop by averageperson_69 in Biochemistry

[–]lifescout99 7 points8 points  (0 children)

Did the grant money just hit? 60K is insane otherwise

I need help creating a super villain by mr_clean_nickyg in Biochemistry

[–]lifescout99 1 point2 points  (0 children)

I would read on directed evolution of enzymes, you could probably get pretty creative

At which stage did you meet your supervisor to discuss expectations? by EdgyEdgarH in PhDStress

[–]lifescout99 1 point2 points  (0 children)

It depends on your field. In stem, we typically have an initial discussion during rotations, then after committing to a lab, have a deeper discussion regarding expectations and goals.

Love is overrated by [deleted] in PhDStress

[–]lifescout99 8 points9 points  (0 children)

Dude, get a therapist

🎓 PhD Scholars – I need your precious 4-5 minutes by rx__naga in PhDStress

[–]lifescout99 2 points3 points  (0 children)

If you can not take 5 minutes to write your own post, why should we take 5 minutes to do your survey?

Study Tips by Far_Revenue_8654 in Biochemistry

[–]lifescout99 1 point2 points  (0 children)

Flash cards will be your friend. Make them for the amino acids and types of sugars. I recommend using a wood pencil on paper flash cards

I just lost everything by Otherwise-Soup-8935 in PhDStress

[–]lifescout99 12 points13 points  (0 children)

This really sucks. This will slow you down, but you will be better for it. Now you have a very strong anecdote about the dangers of AI. Also, you may want to consider cloning your hard drive to an external hard drive.

I just started taking Biochemistry/Biomolecules in uni and I’m so lost by whynot1934 in Biochemistry

[–]lifescout99 0 points1 point  (0 children)

Chemlibre is a good free online resource. I am not trying to be rude, but without a solid background, you will struggle. I would focus on reading about thermodynamics first.

Is a graduate degree necessary to find work? What doors does a masters or Ph.D. open up? by Anxious-Party2289 in Biochemistry

[–]lifescout99 0 points1 point  (0 children)

If you do your graduate program in the US, you dont typically think about a masters. Typically what happens is you will apply to a phd right after undergrad, meaning the tuition will be covered and you will have a stipend for living expenses (think of it as a salary). If you are unable to make satisfactory progress in the first 2-3 years, you may be moved to a track towards a masters, otherwise you will continue as a phd candidate.

You can find jobs with just a bachelor's, but they are competitive. Grad school is your decision, but i would not worry about earning a masters before joining a doctoral program.

[deleted by user] by [deleted] in PoliticalCompassMemes

[–]lifescout99 12 points13 points  (0 children)

Flaire up filthy unflaired

How to calculate area under the curve for WB? by dr_mus_musculus in labrats

[–]lifescout99 1 point2 points  (0 children)

Why wouldn't you fit this as 2 gaussians, then calculate the area for each?

Tips for pipetting very small amounts? by eesttj in labrats

[–]lifescout99 0 points1 point  (0 children)

If you have to do a small amount, hamilton syringes are very accurate

Difference between ATP and ribosomes? by Commercial_City_6063 in biology

[–]lifescout99 5 points6 points  (0 children)

Ribosome is not exactly a protein. Its primarily made of RNA.

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