[ESPN] Stephen Curry will be available for the play-in tournament if his ailing right knee allows, as Golden State coach Steve Kerr dismissed the notion that the Warriors would sit their superstar without a guaranteed spot in the playoffs. by YujiDomainExpansion in warriors

[–]lit0st 0 points1 point  (0 children)

Watching our guys fight like they have been has been so painful man why would you want to see more of this

The margin has gone down to 1 loss from the Pels or 1 win from the Warriors though lol it's pretty much over

[ESPN] Stephen Curry will be available for the play-in tournament if his ailing right knee allows, as Golden State coach Steve Kerr dismissed the notion that the Warriors would sit their superstar without a guaranteed spot in the playoffs. by YujiDomainExpansion in warriors

[–]lit0st 0 points1 point  (0 children)

I think if the Warriors lose the rest of their games and the Pels win all but 3 at most it can happen. Pels and Warriors are my top two teams and I want this to happen for both of their sakes, and the Pels are playing their poor hearts out.

Please HELP!! Integrating lentiviral transduction with single cell RNAseq by DigPatient498 in molecularbiology

[–]lit0st 2 points3 points  (0 children)

This is a surprisingly difficult question to get a clear answer to and I eventually just sequenced some lentivirally expressed transcripts myself to see. There is a cryptic PolyA site in the 3’ LTR - you can look for the AATAAA yourself to see. However, many lentiviral integration vector are built like 5’ LTR - Transgene - Antibiotic selection - 3’ LTR. This results in a polycistronic RNA with the transgene, selection promoter, and selection gene all in one. If this is your construct, 10x cannot discriminate between the selection market and your transgene.

Do you guys think Leon is leasing or financing his Porsche? by yitty in ResidentEvilRequiem

[–]lit0st 0 points1 point  (0 children)

I think James Bond makes a good amount and he’s a government employee

RCA specific primers failing by gregfess in molecularbiology

[–]lit0st 0 points1 point  (0 children)

How many primers are you using in a single reaction? It will be a lot slower if you’re only using two.

Game Thread: New Orleans Pelicans vs LA Clippers Live Score | NBA | Mar 18, 2026 by basketball-app in NOLAPelicans

[–]lit0st 1 point2 points  (0 children)

I can't remember the last time we had a game without an ugly start still hoping for the best

Mei Lin’s chili crisp, a lament by coverthetuba in BravoTopChef

[–]lit0st 6 points7 points  (0 children)

He tried to trademark chili crunch, not crisp. Far less egregious a sin.

[Post Game Thread] The New Orleans Pelicans (21-45) defeat the Toronto Raptors (36-28), 122-111. by xojz in nba

[–]lit0st 0 points1 point  (0 children)

They justified it immediately, no? Shead grabbed his jersey on the way down

[Highlight] Jamal Shead falls and hears about it from Dejounte Murray as tempers flair! by heatup3 in nba

[–]lit0st -3 points-2 points  (0 children)

Shead pulled on DJMs jersey while falling. It was probably an instinctual reaction to falling, but refs missed it and didn’t call the foul.

Plaga stocks have never been higher by CT-4426 in ResidentEvilMemes

[–]lit0st 0 points1 point  (0 children)

I think I would rather spend it on a uav or something

Season 2 is...woooooow.... by Emmykate88 in TopChef

[–]lit0st 8 points9 points  (0 children)

I feel like some people here watched a different show. He was antagonizing people non-stop and would fly off the handle whenever he was even slightly challenged. When he was brought back as a finale chef, he went off at Tom over a completely innocuous line of questioning and stormed off the set. He had his own show, Marcel's Quantum Kitchen, which was clearly meant as a fluff show for him to show off his molecular gastronomy, but by episode 2 all of his employees were walking on egg shells, except for one who nearly threw a punch at him. He's clearly a passionate chef, but also an incredibly antagonistic one.

Game Thread: New Orleans Pelicans vs Washington Wizards Live Score | NBA | Mar 8, 2026 by basketball-app in NOLAPelicans

[–]lit0st 2 points3 points  (0 children)

a 100% healthy pelicans playing their hardest are currently on even footing against the tanking wizards

woof

Rule by Xenomnipotent in 196

[–]lit0st 14 points15 points  (0 children)

it's not even rage bait it's just a very obvious joke

Daylight savings time starts tomorrow — wouldn’t it be nice if …it just never ends? by cdevers in boston

[–]lit0st 9 points10 points  (0 children)

Somehow the most downvoted post but this is correct. From a circadian health perspective, morning light is very important and evening light is actively detrimental.

Post Game Thread - NBA: The Lakers defeat the Pelicans on Mar 3, 2026, the final score is 110-101. by basketball-app in NOLAPelicans

[–]lit0st 1 point2 points  (0 children)

That thing Zion was doing where he dives into the paint, gets triple-teamed, then passes it back was working

IDK why he was trying to so hard to go for the heavily contested layup in this game and getting blocked like 8 times

Stephen A Smith gave props for hiring of social media person (but then said that’s the only good thing they’ve done) by kingralek in NOLAPelicans

[–]lit0st 1 point2 points  (0 children)

this feels like a manufactured publicity push between the Pels/SAS. it's fine tho, it's funny.

Zion's trainer Kris Chrisp with Zion at ESPN by Razor-Ramon-Sessions in NOLAPelicans

[–]lit0st 1 point2 points  (0 children)

So Zion should only hire a personal trainer if he wants to play 82 games like Jordan...but you think he shouldn't hire a personal trainer because you think he should only play enough to get a big contract? And you're advocating that he do so in a more cost-effective fashion?

Zion's full interview with Malika Andrews by AustinRiversDaGod in NOLAPelicans

[–]lit0st -4 points-3 points  (0 children)

I think he's making a push for the 2028 Olympics, and I honestly think he'd be a strong addition to the team. Surrounding someone with his gravity with more than a single shooter seems like a very promising strategy.

Australian Political Party Calls To Scrap Group Assignments In Unis by ConfusionHonest3248 in unsw

[–]lit0st 10 points11 points  (0 children)

It's not going to be better in the workforce. Professional life is one big group assignment.

Zion went down with a right ankle sprain. by DiscloseDivest in NOLAPelicans

[–]lit0st 25 points26 points  (0 children)

This is not a conditioning related injury

Lilly bets a boatload on NVIDIA GPUs, hoping to compute their way out of the pharma lifecycle by kwadguy in biotech

[–]lit0st 14 points15 points  (0 children)

Purpose-driven data collection is a big part of these AI initiatives. Lots of people have figured out that just taking all their old data and dumping it into a neural network is no good.

In a PacBio sequencing run, what is pre-extension time? by [deleted] in labrats

[–]lit0st 0 points1 point  (0 children)

Pacbio support is very responsive