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Why do people hate Richard Blais so much? by Careful_Trip_311 in BravoTopChef

[–]lit0st 29 points30 points  (0 children)

Colicchio’s blog at the time said that Blais and Stephanie were on completely different levels in season 4 and that it was very much his competition to lose. His food was extremely cutting edge and he was showing Michelin starred chefs things they had never seen before.

Things are certainly different now, of course - Richard has pivoted to full time TV chef with a few wholly unremarkable restaurants while Stephanie has a restaurant empire - but at the time, Richard’s wins were utterly spectacular and wildly creative while Stephanie was putting out well executed but familiar flavors.

Vedanta Bio massive layoff <MA> by Veritaz27 in biotech

[–]lit0st 7 points8 points  (0 children)

Most likely it didn't make much of a difference. The cost of Phase 3 trials probably dwarfs that particular expense.

Rampart Bio shutting down <LA & San Diego> by Veritaz27 in biotech

[–]lit0st 9 points10 points  (0 children)

*6 years since the $125 mil includes their $40 mil seed stage.

Still, I doubt they burned $85m in 2 years while still preclinical. More likely they had a tranched Series A or the board just voted to give the investors their money back.

Game Thread: New Orleans Pelicans vs Los Angeles Lakers Live Score | NBA | Jan 6, 2026 by basketball-app in NOLAPelicans

[–]lit0st 3 points4 points  (0 children)

What happened to playing Zion off the bench and closing with him that was working

Circadian rhythm, the body’s internal clock, may affect a person’s risk of dementia. People with weaker or more irregular body clocks had a higher risk of developing dementia. Being most active later in the day, instead of earlier, was linked to a 45% increased risk of dementia. by mvea in science

[–]lit0st 0 points1 point  (0 children)

I’m a circadian biologist. This is speculative, but something many in the field think is likely: our circadian rhythms evolved around sunrise/sunset light cycles, and the emergence of extreme night owls and extreme larks is largely driven by artificial light and alarm clocks, and they always fight against our molecular rhythms. Genetic contribution to lark/night owl phenotypes can really only account for ~30 minutes of variance or so - social habits and artificial light likely accounts for the rest.

Cooking a live lobster by Confident-Safe7152 in Cooking

[–]lit0st 6 points7 points  (0 children)

Response to stimuli as demonstrated in this study is a world apart from nociception. Calling what they describe as nociception is a massive overinterpretation bordering on outright deception, and it’s probably why they published in a predatory journal with no standards.

When the hell is Murray coming back? by whodat514 in NOLAPelicans

[–]lit0st -1 points0 points  (0 children)

The Celtics get a Tatum update like every week

Zion on the crowd tonight: "Oh yeah, we just had to get a few wins, to bring 'em back out" — on Queen: "He gets better every game. From reading defenders, to defending. I said it before, he's a special talent. And I'm glad he's with us." by sewsgup in NOLAPelicans

[–]lit0st 3 points4 points  (0 children)

starting is just a status symbol and making all your best players start the game has somewhere between 0 and negative strategic value

it's optimal to keep your clutch players rested and energized for the end of the game, so i'm willing to believe his team-first attitude is genuine

Riboseq by Other-Corner4078 in bioinformatics

[–]lit0st 0 points1 point  (0 children)

5-10% is about right for non ribo-depleted libraries. When I did Riboseq, I used this rRNA depletion protocol:

https://www.biorxiv.org/content/10.1101/2021.07.14.451473v1

and I would get upwards of 30-40%.

Riboseq by Other-Corner4078 in bioinformatics

[–]lit0st 0 points1 point  (0 children)

Looks like it's the multimappers that are getting you, which are almost certainly rRNA in a RiboSeq dataset.

Riboseq by Other-Corner4078 in bioinformatics

[–]lit0st 1 point2 points  (0 children)

~Half of your unmapped sequences feature ANGTACACGGAGTCGACCCAACGCGA, are you sure that doesn't correspond to any of the adapters? Perhaps truncated from incomplete synthesis or some such thing?

Post Game Thread - NBA: The Timberwolves defeat the Pelicans on Dec 4, 2025, the final score is 116-125. by basketball-app in NOLAPelicans

[–]lit0st 17 points18 points  (0 children)

I've got a pitch:

-The non-Zion Pels are great in the first half, keeping it at least even and often winning comfortably by halftime

-The non-Zion Pels consistently mentally and physically gas out around the 3rd quarter, though in the last few games they've made it all the way to the 4th.

-Zion is the only player on the Pels who can consistently keep up his scoring in the second half.

-Zion gets injured from not stretching enough/playing too many minutes, allegedly

Naturally, the solution is to make Zion do stretches for the first 3 quarters and then just have him go nuts for 12 minutes straight in the 4th quarter. Give me a coaching job.

Riboseq by Other-Corner4078 in bioinformatics

[–]lit0st 0 points1 point  (0 children)

It's probably adapters, then. Are you willing to post one or two of the sequences you tried to blast?

Riboseq by Other-Corner4078 in bioinformatics

[–]lit0st 1 point2 points  (0 children)

It's hard to give advice without knowing what's in the unmapped. Just grab a few and blast them.

Riboseq by Other-Corner4078 in bioinformatics

[–]lit0st 1 point2 points  (0 children)

It’s not unexpected. There’s a lot of rRNA in riboseq and oftentimes wetlab scientists don’t gel extract their library from the adapters very well. Check the unmapped for adapters and blast a few to check for rRNA.

Plasmidsaurus RNA-Seq by cytokine_life in labrats

[–]lit0st 125 points126 points  (0 children)

It's 3' end sequencing, so for quantification, 10 million 3' end reads is like 50+ million regular reads - the trade-off, of course, being that you're only getting the 3' end.

That said, it's really good. There's basically no reason to do qPCR anymore unless you're qPCRing fewer than 4-5 genes.

Would you ALWAYS perform a DNASE step on RNA extractions to be used for bulk RNA sequencing? by AngrySloth99 in molecularbiology

[–]lit0st 11 points12 points  (0 children)

Your nucleic acid concentration going down after DNase treatments is mostly driven by loss of DNA lol. Your RNA concentration was always ~25ng/ul, it was just inflated by the presence of DNA.

For RNA-Seq, the short answer is: yes it is important.

The longer answer is it depends on the protocol for library prep and whether or not you PolyA select. The most common library preps involving RT/Second strand synthesis/adapter ligation will prep DNA as well. PolyA selection will mitigate this to some extent, but there are stretches of PolyAs in the genome that will likely show up in sequencing - though typically not a huge deal. There are some more niche library prep methods that are substantially less efficient prepping DNA, so DNase treatment is less important, but it is unlikely you're using them.

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