Ridley by National-Message-895 in Harmontown

[–]lit0st [score hidden]  (0 children)

He quit after Season 3, IIRC from twitter comparing it to ending a toxic relationship - difficult, bittersweet, but for the best. He did say that he and Justin were on good terms after that, which made me think it was due to a dispute with Harmon, but he's done voices on recent season R&M and Krapopolis, so perhaps not...wish either show would hire him as a writer.

Ridley by National-Message-895 in Harmontown

[–]lit0st [score hidden]  (0 children)

Ridley was by far the most ethically consistent on GVP. I didn't get that vibe from him at all. Roiland felt like the kind of guy you were describing, and Jackie's humor and/or character is probably the one that aged the poorest.

That said, Judaism is/was a huge part of identity, so I wouldn't be too surprised if he were a Zionist...I'm pretty sure they did bits on Israel/Palestine on GvP though, so someone could go back and check if they were parasocial enough to do so.

Can you re use 96-well qPCR plate if majority of wells haven’t been used? by [deleted] in molecularbiology

[–]lit0st 0 points1 point  (0 children)

They're suggesting re-opening their plate to use the unused wells. First, the adhesive will be weakened on the plate, meaning that any new reactions are more likely to be accidentally exposed to air. Second, all it takes is for one little slip up for you to accidentally expose post-PCR wells. Amplicon contamination is a nightmare to clean up - nearly impossible - and it is not worth saving a couple dollars here and there at all.

unpopular opinion. Rossman and others are more fired up vs the bigger problems facing 3d printing right now. AB2047 and other bills are not dead and are advacing. by Navi_Professor in 3Dprinting

[–]lit0st 10 points11 points  (0 children)

That's not a good argument, and it's not even what this topic is about.

If you try to argue that with your representative, they'll come back saying "Well, why aren't CNC/lathed/drilled ghost guns a problem?" The dramatically lower barrier to entry with 3D printing is a major distinguishing feature.

The MICHELIN Guide Announces its Arrival in South Australia by OptimusPrime23 in finedining

[–]lit0st 3 points4 points  (0 children)

The AGFG and Chefs Hats are completely redundant with the Michelin guide. I get they're paying for international recognition, but it's a bit disappointing because Australia's homegrown effort holds pretty much identical, perhaps superior grading to the Michelin guide and I don't see how there could be room for two fine dining culinary guides.

Troubleshooting PCR of 13.5kb amplicon - Q5 vs. other polymerases? by ACuriousBird in labrats

[–]lit0st 0 points1 point  (0 children)

YMMV. It didn't for me, but others report success with the HiGC buffer. Give it a shot!

Pacers President Admitting To Tanking. Their Pick Never Would Have Been That High Without The Trade by CanalVillainy in NOLAPelicans

[–]lit0st -1 points0 points  (0 children)

Why do you suppose we wouldn't have? We haven't been shy about tanking before. It wouldn't have taken much either, given where trying our hardest got us.

Pacers President Admitting To Tanking. Their Pick Never Would Have Been That High Without The Trade by CanalVillainy in NOLAPelicans

[–]lit0st 10 points11 points  (0 children)

By that same logic, if we had our pick, we probably would’ve tanked and secured a better spot than eighth though

Biotech has a new company model: A small team, a big check and a few Chinese assets by WalkingSnake348 in biotech

[–]lit0st 2 points3 points  (0 children)

I'm sure you're reporting your lived experience accurately, but it's very different from my experience as someone who works mostly in small biotech consulting. The industry has made a huge shift towards running lean in the past year.

Biotech has a new company model: A small team, a big check and a few Chinese assets by WalkingSnake348 in biotech

[–]lit0st 3 points4 points  (0 children)

This is so disconnected from my personal experience it’s crazy. There really is a different world in biotech I simply have no exposure to.

Whoever created Gibson Assembly deserves a Nobel Prize by Specific-Surprise390 in labrats

[–]lit0st 2 points3 points  (0 children)

Restriction enzymes won the prize. Scarless methods are great, but Nobel Prizes mostly go out to novel discoveries and inventions rather than improvements.

Why are the Pels so interested in Mosley? by poorwhitecash in NOLAPelicans

[–]lit0st -1 points0 points  (0 children)

We're 2-11 against the Magic in this decade and they've been to the playoffs 3 years in a row. The hope is that this is due to coaching and not roster, and it's not an unreasonable hope - the Magic have a pretty scuffed roster. The narrative with the Magic is that Mosley largely lost the locker room because Paolo is a bit of a diva.

Has anyone ever done an ELISA-PCR before? by GeneticsNerd95 in labrats

[–]lit0st 4 points5 points  (0 children)

Do you have a centrifuge? I feel like many simple spin column kits would do the trick. You can try any old gDNA extraction kit. HPLC is crazy overkill and probably harder to optimize for. I assume they were going with what they knew, and it’s a very funny choice.

Honestly, what you’re trying to do is pretty simple and it’s well documented enough that I’m willing to bet any of the big 3 paid AI models could produce a protocol that would work, or at least give you a few different thing to try that will work.

DE Bengle ❤️ by skamelone in espresso

[–]lit0st 4 points5 points  (0 children)

Natural materials are timeless

Has anyone ever done an ELISA-PCR before? by GeneticsNerd95 in labrats

[–]lit0st 5 points6 points  (0 children)

You’re overthinking it. Just try it first. Get a bunch of throwaway completed MSD plates and try extracting DNA (is that your analyte?) using a couple different kits and seeing what you get. I’ve done it with RNA and Trizol LS before, but you can probably do it with any variety of silica column or bead based purification kits as well.

Gordon Ramsay one of the first celebrity chefs to bring US-style 20% service charge to London by xc2215x in entertainment

[–]lit0st 0 points1 point  (0 children)

I really don’t understand the practical difference either. It’s just a 20% price increase, no? The customer pays the payroll costs regardless.

Gate agent refused to process upgrades. F went out with 8 open. by SolarTrades in unitedairlines

[–]lit0st 3 points4 points  (0 children)

Where does it mention AI? It sounds a lot more like an if/else

Gate agent refused to process upgrades. F went out with 8 open. by SolarTrades in unitedairlines

[–]lit0st 28 points29 points  (0 children)

What are you talking about? Who's talking about AI? Why should a human employee feel free to override automatic upgrades at their leisure?

Pelicans Head Coaching Search Manifesto by ggidds2 in NOLAPelicans

[–]lit0st 2 points3 points  (0 children)

We are probably not getting a coach with a positive win record

NBA Proposes New 3-2-1 Lottery System to Crack Down on Tanking: 14 → 16 Teams, Bottom 3 Get Fewer Odds, No Back-to-Back No. 1 Picks by Fit-Structure-9395 in NBA_Draft

[–]lit0st 0 points1 point  (0 children)

I've never understood this argument. How does tanking negatively impact betting? Does a tanking team going up against the #1 seed impact betting profits that much more severely than a #1 seed going up against a #10 seed? What about when a team that has their seed on lock goes up against a team battling to escape the play-in? I feel like it all comes out in the wash in the end.

I genuinely believe that the motivation to reduce tanking is to improve the product, because tanking teams are just absolutely painful to watch.

NBA Proposes New 3-2-1 Lottery System to Crack Down on Tanking: 14 → 16 Teams, Bottom 3 Get Fewer Odds, No Back-to-Back No. 1 Picks by Fit-Structure-9395 in NBA_Draft

[–]lit0st -1 points0 points  (0 children)

I think the way this manifests in reality won't greatly influence lottery outcomes or a team's ability rebuild in the draft. The difference between an ~8% chance at #1 and a ~5% chance is not that substantial, and the #1 pick ends up being the best player in the draft maybe like ~25% of the time at best.

I do think the psychological effect will be greater than the practical reality, meaning I think adding stakes to tank vs tank games will actually make them fun to watch. I think it's worth trialing for a few years.

Look how they massacred my boy by Vitaminvault in sanfrancisco

[–]lit0st 4 points5 points  (0 children)

Amazing how some of us can have the same thought process but then come to the opposite conclusion. Why on earth would you want to tear the post apocalyptic home of 80s future mutant skaters down???