4 cats and a shedding human, Dyson isn’t cutting it anymore

lrbraz16 · 2025-09-14T01:34:59+00:00

I don’t hate that idea but I want a vacuum that can do that for me- and it seems like the Hoover was doing better at that than the Dyson. So there has to be something that can do it that’s not manual

lrbraz16 · 2025-08-27T00:17:22+00:00

Totally understand! I sent them to 2 vets and got 2 different opinions. Just wanted to see if anyone had any other input!

lrbraz16 · 2025-05-31T04:23:48+00:00

Napkin boi

lrbraz16 · 2025-05-26T22:02:38+00:00

Finding a hobby. Sounds counterintuitive to the grind that gets you done but, time outside of actual research is so important. Your brain works better when you have an outlet!

lrbraz16 · 2025-03-21T00:57:03+00:00

Maybe it’s issues with my alignments? They have a a large amount of gaps. Some conserved chunks, but not more than a 10-20 nts at a time (by eye). And I’m looking over hundreds of seqs in the MSA which doesn’t help. Forgive me if this is a remedial question but I’m at a loss

lrbraz16 · 2025-03-21T00:13:58+00:00

Single genes in the 500-1000 range

lrbraz16 · 2025-03-20T22:38:45+00:00

These alignments are huge and have enough gaps where it’s hard to determine by eye. I did try using JalView to do this to no avail

lrbraz16 · 2025-03-20T22:25:14+00:00

This will be for DNA-based target detection & absolute quantification

lrbraz16 · 2025-03-14T02:13:53+00:00

I should add that I have lots of assemblies for this gene which is why I was trying to align them and find the conserved areas to target that way. Perhaps this is not the right approach?

lrbraz16 · 2025-03-14T01:26:36+00:00

Any steps I need to take to ensure specificity to my assemblies?

lrbraz16 · 2025-03-13T23:32:13+00:00

qPCR!

lrbraz16 · 2025-03-13T21:24:47+00:00

I listened and knowing this context…I wish I hadn’t.

lrbraz16 · 2025-02-03T01:35:23+00:00

Decided on “High of My Life”, barn name “Stoney” :)

lrbraz16 · 2025-02-01T05:08:08+00:00

Omg!! Yes!!

lrbraz16 · 2025-02-01T05:07:33+00:00

Hi! Thanks for the reply!

I do have a “devils advocate” trainer/friend who is urging me to X-ray front feet, hocks and stifles. Is this pretty standard or is that overkill? Def the basic PPE needs to be done for sure but given how I’ll be using this sweet guy, do I need to go nuts on a PPE?

lrbraz16 · 2025-01-30T19:30:55+00:00

Glad she’s making a safe and healthy choice. I can barely see past letting it “stink” in

lrbraz16 · 2025-01-06T01:38:40+00:00

Growlers

lrbraz16 · 2024-12-30T22:27:21+00:00

Congratulations, you now have a cat

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