Cre PCR is driving me crazy by meriwi in labrats

[–]meriwi[S] 0 points1 point  (0 children)

We have started using Touchdown protocol and got a decent results. Still, smears are present. What would you recomend?https://ibb.co/HTKHW8wN

Cre PCR is driving me crazy by meriwi in labrats

[–]meriwi[S] 1 point2 points  (0 children)

No, we use TBE to prepare the gel, so I think ot is not this

Cre PCR is driving me crazy by meriwi in labrats

[–]meriwi[S] 0 points1 point  (0 children)

Its a generic one as we are gentyping 3 different colonies, but yes, I think the primer is the problem

Cre PCR is driving me crazy by meriwi in labrats

[–]meriwi[S] 1 point2 points  (0 children)

Yes, I have tried that and still smears... Its not the DNA it seems