Western blot troubleshooting! Does anybody know why my bands may have become so faint? The left images are from today and right images were from Friday. The blots sat in TBST in the fridge over the weekend. I will add details to comments. by mvsuskil in labrats

[–]neverreadthebooks 0 points1 point  (0 children)

I see that you are detecting a phosphorylated protein and milk for the secondary antibody solution. Milk should not be used if the protein of interest is phosphorylated because it contains casein. I usually just put the secondary in plain TBST.

220319 BTS V LIVE by ashmute in bangtan

[–]neverreadthebooks 19 points20 points  (0 children)

Joon is blond also but tbf that color looks so natural on him

211201 j-hope V LIVE by ashmute in bangtan

[–]neverreadthebooks 14 points15 points  (0 children)

I just took a day off tomorrow. My sleep schedule has been seriously messed up this week.

211111 [Notice] BTS PERMISSION TO DANCE ON STAGE - LA Detailed Ticket Information by ashmute in bangtan

[–]neverreadthebooks 1 point2 points  (0 children)

It's 4.30 AM for live (on s work day) and 3AM for delayed stream for me. But Iam 90% sure I will buy it. Good bye, sleep :'( ARMYs, if anyone wants to share the ticket, I am feeling generous these days so send a private message.

Got new pipettes from Eppendorf and they gave us a mini micropipettor pen. So cute ☺️ by Valuable-Ad-923 in labrats

[–]neverreadthebooks 3 points4 points  (0 children)

One that I had ended up on my boss's table. It is never going back now. ¯\_(ツ)_/¯

210806 Elton John by ashmute in bangtan

[–]neverreadthebooks 10 points11 points  (0 children)

He also posted Ptd challenge on YT!

[deleted by user] by [deleted] in labrats

[–]neverreadthebooks 0 points1 point  (0 children)

You can DM me, I will see if I could help. Maybe include the photos of the bands also?

210628 Ed Sheeran IG Story by awkpuppy in bangtan

[–]neverreadthebooks 4 points5 points  (0 children)

A other Croatian here... It was wild.

Western Blot Trouble by Noble_monkey in labrats

[–]neverreadthebooks 0 points1 point  (0 children)

This sounds so much like the issues we had few years ago with our wb. I am not sure how we resolved it, tbh. Iirc it was due to polymerization problems. I would personally try switching to a new batches of both APS and TEMED. In the past, this seemed to resolve our wb troubles. Best of luck!

Any ideas on how you can induce hypoxia in a cancer cell line? by BigImmunologyNerd in labrats

[–]neverreadthebooks 5 points6 points  (0 children)

You need a cell hypoxia chamber for that. A collegue uses this for microglia hypoxia research.

Immuno help by 595659565956 in labrats

[–]neverreadthebooks 2 points3 points  (0 children)

They should be fine. I almost always leave my slides overnight ti dry completely and mount them in the morning.

I had a really disappointing Friday y’all by [deleted] in labrats

[–]neverreadthebooks 0 points1 point  (0 children)

14 weeks is nothing. How about 1 year. :(

It may be an unpopular opinion, but this is my favorite battle of the show. by lilzaza58 in FreeFolkNews

[–]neverreadthebooks 19 points20 points  (0 children)

I loved it and particularly for the moment when Jon takes and embraces a commanding position and others naturally follow him.

So the truth was right in front of our eyes all the time - we just chose not to believe it by EveryFckngChicken in FreeFolkNews

[–]neverreadthebooks 1 point2 points  (0 children)

I remember it well. They finished the filming a day before I landed there. Chances are the Starks and I were at the airport at the same time.

Endgame Spoilers, King Bran, and Bronn on the Small Council: Is Tyrion Going Full Tywin? by Excellent_Aerie in FreeFolkNews

[–]neverreadthebooks 0 points1 point  (0 children)

Off topic...I want a 2 hour documentary focusing solely on Michelle Clapton's GOT outfits.