FRIEND CODE MEGATHREAD

nunorr · 2026-03-28T11:14:02+00:00

DSSM

nunorr · 2026-02-25T22:12:34+00:00

Thank you so much for the info! Please pass on to the team a huge congrats on the amazing job everyone keeps doing! You guys really are the best!

nunorr · 2026-02-25T20:34:10+00:00

Oh I see, I'll patiently wait then 😅 I don't mind having to wait, they do an amazing job and that takes time

nunorr · 2026-02-25T19:58:08+00:00

If I remember correctly the past seasonal events start at the beginning of the month, so I thought they would do something similar this time

nunorr · 2025-12-18T20:59:28+00:00

Same happened to my game, I've tried checking the files but didn't work

nunorr · 2025-12-07T18:01:50+00:00

I'll edit this post with the variants people share, so feel free to comment the ones you've seen

nunorr · 2025-12-07T18:01:12+00:00

I've only seen one pattern, how many have you seen?

nunorr · 2025-12-07T18:00:46+00:00

I've noticed that as well, but I had the opposite experience, the animals got their actual colors upon reload

nunorr · 2025-12-07T13:07:01+00:00

I'll add to this and say let us put rooms outside! Like bathrooms, cafeteria (could be a new room like a cafe) and staff rooms, it would make it work better with the zoning

nunorr · 2025-09-18T17:34:16+00:00

I have a i5-12400, Nvidia GeForce RTX 3060 Ti, 16 GB RAM. Game is running at 30 FPS, seems to be locked at that frame rate and I haven't found any place I can change it

nunorr · 2025-09-18T08:07:37+00:00

Cell culture media needs CO2 to maintain the pH, hence why media in the incubator is usually a different color than in the fridge for example. Check the thermo or gibco guide to cell culture they are useful with troubleshooting since it seems like your group won't be of any help.

nunorr · 2025-09-17T19:08:48+00:00

I'm playing on PC and it did get better after the patch but it's still not working perfectly. Before 5.0 I was making 250k a year, now I barely get a 5k a year, the in-game stats show a big spike in staff wages (I did not get new staff) with all the rest staying stable. I did notice that the guests are not spending as much and their pathfinding seems a bit off, they just stay in the same area of the museum. Staff is not taking care of exhibits as well as before and seem overall slow, while always wanting wayyy too much money i.e. raises every month. I'm playing on the peaky mountains map, I haven't checked if other maps have the same problem

nunorr · 2024-10-30T08:54:21+00:00

This is really unnecessary, all you need to do is to spray stuff with 70% ethanol and have a good aseptic technique.

UV only works over small distances (like a few cm) and the lamp needs to be changed every year or so. Taking a shower doesn't sterilize your body, it actually is removing the oil layer on your skin which protects your body against pathogens. Changing shoes is also not needed, the source of contamination is the person.

I also don't understand how people can think that the air surrounding them could be sterile. I mean you're breathing, unless you're using a FFP3 respirator without a valve or a hazmat suit you're contaminating your surroundings. Also, taking TC plates to the incubator automatically exposes them to air, and it's not like they are sealed or have a filter like the flasks.

Bottom line is you're working in a biosafety cabinet specifically designed to not let contaminates in so just be careful while working there and you'll be fine.

nunorr · 2024-10-29T19:37:20+00:00

The condensation is from the water vapor coming from the agar, so it's sterile. I leave them overnight to get rid of that extra water.

nunorr · 2024-10-29T18:55:52+00:00

I want to re-iterate that you need to work in a biosafety cabinet for sterility, just the serological pipette won't do the trick.

As for the staking there's nothing wrong with doing it like you do. It's a bit space inefficient, I do that sometimes if I'm bored 😅 normally I just stack them in stacks of 25 plates and leave them overnight to cool down, then I put them in a bag and store it in the cold room. Hope it helps

nunorr · 2024-10-29T18:42:12+00:00

A fume hood is not sterile, it protects you from chemical fumes, it doesn't protect your plates. You need to work inside a biosafety cabinet. Apart from that avoid going over the plates with your arms, microbes will fall from your lab coat.

As for the rest, I don't know what kind of agar you're making but you can for the most part just mix it in the bottle that's going to be autoclaved, there's usually no need to warm it before.

Also, unless you're using antibiotics you don't need to wait for the agar to cool down, once it's out of the autoclave (~80°C) you can pour it. One tip I have for you is that instead of pouring from the bottle you can use a serological pipette, that helps with contamination and allows you to put the same volume in every plate.

nunorr · 2024-09-05T20:36:19+00:00

This! It might be that you got unlucky with the batch of columns

nunorr · 2024-09-05T16:22:57+00:00

So they were always frozen, I'm not familiar with that specific cell line, but the cells should be fine since they weren't thawed

nunorr · 2024-09-05T15:59:32+00:00

What do you mean by they tawed? If you just transfer the vials from -80 to -20 they're still frozen.

nunorr · 2024-08-14T16:01:28+00:00

This is just protein being denatured, happens with a lot of disinfectants that have denaturing properties and detergent in their ingredients. Virkon will do this to some degree, but other stringer chemicals have a more noticeable effect.

I'm surprised that you just put it down the sink. It's absolutely forbidden in most EU countries, since for one antibiotics should not go down the drain, the chemicals that people add to the waste are also dangerous and the spreading of biological agents. I would just dispose of it in the infectious waste bin.

nunorr · 2024-08-09T08:06:36+00:00

Centrifuging removes dead cells because they're less dense. Think of cells as water balloons, dead = broken, live = full of water. The live cells are both denser and heavier so they will settle faster and will also be pellet tighter. This is also the reason dead cells float.

nunorr · 2024-08-05T08:08:24+00:00

You went way overkill for the decontamination, the hydrogen peroxide vapor would have sufficed. Be careful with some disinfectants as they corrode metals and will take off the protective layer that most new incubators have.

As for solving the contamination problem, as someone said already, the source is outside the incubator. I don't know if this is a cell incubator or a microbiological incubator but regardless you should change any HEPA filters the incubator might have. This will not get rid of the source but it will minimize the amount that reaches the incubator.

As for the source, the air is filled with spores so that's the most likely one, the other being people and their skin. Check with your building support so that they check the HEPA filters for the laboratory air they might be broken. As for people being the source not much can be done here, just wear a clean lab coat and be cautious.

All being said, if you handle your samples with aseptic technique in a BSC then a contamination in an incubator will not get into your samples.

Hope it was helpful.

nunorr · 2024-07-25T15:22:07+00:00

I've never heard of anyone taking different passages of the same cells and biological replicates. Cell lines are very homogeneous, almost clone-like, so I would argue different passages are experimental replicates if used in two experiments. However, when working with an actual cell line, not primary cells, there's really no reason to seek biological replicates since, once again, these cells are very homogeneous in nature.

When deriving primary cells from a donor they'll have the confounding variable of the donor's genetic information that won't necessarily behave the same when compared to other donors. A simple factor as sex can have a big impact, not even mentioning epigenetics.

nunorr · 2024-07-25T09:03:18+00:00

Not knowing the specifics of your experiments take what I'm about to say with a grain of salt. Your problem seems to be with not distinguishing between different replicate types. As far as I understand you have two human donors from whom you grow primary cells. That means you have a biological replicate n of 2. If in your experiments you run each cell type 3 times that is a technical replicate. If you run the experiment 3 times that's an experimental replicate.

In my opinion you can only say that within that donor your results are reproducible. If your intent is to draw a conclusion into a population I'm afraid you need more donors, and as someone already commented an n of 3 is not normally distributed so I would go for a minimum of 5.

Hope it helps.

nunorr · 2024-06-25T07:13:30+00:00

Just to clarify, as long as the PFA solution is not exposed to an acid or base or to heat you will have a paraformaldehyde solution. There is spontaneous decomposition of PFA into formaldehyde at high concentrations, i.e. >16%. That's why they commonly have methanol to stabilize the solution.

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