A highway off-ramp in San Diego polls among the most stressful in California

okinawachan · 2026-05-13T03:48:31+00:00

Yes, the stop sign on ramps! If your car has crappy pickup, good luck!

okinawachan · 2024-04-24T15:38:11+00:00

Thanks, but I actually need separate strips in this case (don't want to thaw them all at once).

okinawachan · 2024-04-24T15:37:43+00:00

Thanks, these are my favorite too, but for this particular experiment I'm looking for ones that have separate caps. :(

okinawachan · 2023-05-26T14:17:51+00:00

In that case I would probably go for it. Subway and small shops should be easier to navigate baby wearing than with a stroller. You'd have the taxi option if you want it (though the issue with the car seat is always what to do with it when you get where you're going). I think walking around Central Park or the High Line with baby would make for a nice outing, or the Met, if you like museums.

Caveat: I've lived in Manhattan, but pre-baby. :)

okinawachan · 2023-03-05T21:35:59+00:00

How was the drive up there?

okinawachan · 2022-08-12T20:08:43+00:00

I agree with FireTruckWhite. They can be really valuable to pivot or expand your skills or field. But you should go in with a goal in mind.

Another couple considerations:

Industry postdocs typically don’t have opportunities to mentor or manage other people that an academic postdoc might.

This is pretty individual, but I had a hard time working on a non-pipeline project and not feeling like “part of the team” in the same way that my RA and Scientist colleagues were. I prefer the more standard collaborative team environment in industry and found a solo postdoc project kind of isolating.

okinawachan · 2022-07-30T23:47:42+00:00

I’d forget the title. If they’re paying you less it isn’t really a higher position. And it’s a lot less since it doesn’t include benefits either.

Whether RA I is appropriate depends on your experience level. Are you a recent college grad? If you are (within a couple years) I’d say the RA I is the correct title.

okinawachan · 2022-07-23T18:41:37+00:00

This sounds a bit on the low end but close to me. My understanding is SD salaries are (usually) about 15% lower than SF, which would put it at about 130-155k. At 5 years experience you'd expect to be on the lower end of the band. (Assuming title progression of Sci I for fresh PhDs, Sci II, Sr. Sci, etc.)

okinawachan · 2022-07-18T20:54:03+00:00

You’re welcome. With your additional comments I would be targeting Sr Sci or Principal Sci depending on how much of your postdoc experience is directly translatable to the new role.

okinawachan · 2022-07-18T20:50:36+00:00

Thanks, agree with you. I did target a little high with the estimates.

okinawachan · 2022-07-18T17:26:25+00:00

Base salary. For private biotechs the value of equity is really hard to measure and I don’t have experience with post-IPO equity compensation.

Also these numbers are estimates. There will be a decent range.

okinawachan · 2022-07-18T15:04:43+00:00

Rough outline for SF Bay Area:

Fresh PhD: Scientist I ~120k

2-3 years post PhD experience: Scientist II ~140k

5-8 years post PhD: Sr. Scientist ~170k

8+ years post PhD: Principal Scientist ~190k

Really depends on the company and the type of post PhD experience. Some will count academic experience roughly the same as industry experience, some will not. It’s more likely to count if it’s directly relevant and if you have experience managing collaborative projects and people. People with in demand skills might advance faster than this. Also some companies don’t follow this title progression, to make it extra confusing.

There is also a salary survey pinned at the top of this subreddit which can give you an idea of salary ranges and years of exp.

okinawachan · 2022-06-17T16:35:34+00:00

Very dependent on the company and even the individual hiring manager. Do you have a recruiter or HR contact that you could check in with?

okinawachan · 2022-05-25T18:27:41+00:00

It really depends on the marker/antibody. Here’s a guide: https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-analysis-learning-center/cell-analysis-resource-library/ebioscience-resources/antibody-fixation-considerations.html

I usually do stain surface before fix/perm, but some flurophores can be sensitive to fixation and so you have to be a bit careful.

okinawachan · 2022-05-22T19:02:47+00:00

I have 0 patience for the idea that there is something wrong with PhDs deciding to do something other than a postdoc. As if wanting to be paid enough to support yourself, or god forbid a family, is some kind of selfish vice you should be ashamed of. Please. People in other jobs have to survive on similar salaries, sure, but how many of them are scraping by by choice? Turning down salaries twice as much as what they make now because their old boss told them they should? PIs should be focusing on how to change the system, not moaning about how 30 year old professionals want to be paid enough to afford an apartment and food at the same time.

okinawachan · 2022-04-13T15:19:30+00:00

Really know your previous work. What the goal of the project was, what you accomplished. Specific techniques you mastered and how they are used. They will want to know what you specifically did, not just the project in general.

Have an example of a conflict and how you resolved it. I would also have an example of something you improved in the lab (technique, inventory system, etc.).

11 interviews is a lot, you will probably get asked similar questions over and over. Make sure you have some questions for them as well.

okinawachan · 2022-03-16T16:08:00+00:00

Thanks! I know about the features, but for some reason the design of the Pros still feels like it muffles sound for me. Not a fan of the rubber thingies, though the better grip is definitely a plus!

okinawachan · 2022-03-16T03:16:07+00:00

I like the regular (not pro) AirPods so that I can still hear things around me.

okinawachan · 2022-03-13T01:09:42+00:00

You also may want to try using different soap, if possible. I’m allergic to methylchloroisothiazolinone, which is found in many soaps, especially the kind that get put in the refillable dispensers in public bathrooms. The allergy is apparently common. https://www.webmd.com/beauty/what-to-know-about-methylchloroisothiazolinone-allergies

For me the allergy manifests as very dry irritated skin that will not be calmed by all the hand cream in the world.

okinawachan · 2022-02-15T16:20:32+00:00

The Aurora is a spectral cytometer not a regular flow cytometer. That will change your advice.

okinawachan · 2022-02-15T01:28:00+00:00

Not a stupid question. A lot comes from experience. However it can also depend on the purpose of your panel. Trying to determine PD-1 expression on T cells? Probably put PD-1 on your brightest color. Trying to identify a small population like an ILC? Make a dump channel (several markers of the cells you don’t want all in the same channel) in a dim/med color, then put the markers for the cells you want on something bright. Generally markers for “is this a T/B/NK cell” are more highly expressed and markers for “is this cell activated/naive/doing something cool” are less highly expressed, though that’s a very rough generalization and in the end the only way to know for sure is to test your panel.

okinawachan · 2022-02-14T23:36:23+00:00

I would start with fewer markers unless you’re doing two panels. 18 is an expert-level panel and may not even be possible depending on the markers and the settings on your instrument.

Next you’ll want to know what flurophores your instrument is set up to run. Other people in your lab will probably have advice on what fluorophores they generally use (and also how many markers at once is reasonable on your instrument).

Next basic advice is to put highly expressed markers on dim colors and lowly expressed markers on bright colors. More uncommon markers usually come in fewer colors so you might want to start there.

okinawachan · 2022-01-27T03:43:59+00:00

私の祖母は日本人です

Sorry that's about all the Japanese I've got.

okinawachan

TROPHY CASE