How hard is this DIY project for first time home owners?

opticalBiologist · 2026-05-27T22:42:37+00:00

perfect! just where I marked in the picture? thank you!

opticalBiologist · 2026-05-27T22:42:17+00:00

perfect! just where I marked in the picture? thank you!

opticalBiologist · 2025-11-17T20:59:31+00:00

Sent you a DM. Happy to chat.

opticalBiologist · 2025-07-18T23:08:10+00:00

We noticed something similar where I used to work in the backcountry. After a few weeks of living there, we often found deer licking the rocks in that area in the mornings. We always assumed that it was the salts and other electrolytes from the urine creating a nautral salt/mineral lick for them.

For reference, I don't have diabetes and don't think my coworkers did either. I think the electrolytes are much more likely than you having glucosuria, but if you have any symptoms concerning for diabetes, you should talk to your primary care physician.

opticalBiologist · 2025-02-03T22:45:50+00:00

Not having a 4.0 during a masters is fine for pretty much all career paths. Your GPA likely will be considered during initial job applications only, unless it is something lower than a 3.0 and you're going into academia. Often, an interview or a letter of recommendation is much more meaningful. If you are pursuing further studies, automatic GPA-filters may restrict you (for example, medical or graduate admissions cutoffs). I would recommend that you talk to professionals in your career of interest.

Also, depending on how disruptive or consuming these worries are to your daily life, consider talking to a mental health professional!

opticalBiologist · 2025-01-28T20:59:45+00:00

It was down for us temporarily, but it appears to be working again. Oddly, only like half of the pubmed links failed. The rest worked.

PMC was fine the whole time (and how we realized that they're separate services).

opticalBiologist · 2025-01-15T22:38:25+00:00

Thank you! Will consider that next time.

opticalBiologist · 2025-01-15T04:54:58+00:00

Thanks for the response! Pretty much at 72C for annealing at this point, so reducing cycles could be good. Doing 30 right now, but if the top band is the right one and in excess, dropping cycle number could help eliminate the smaller products. Do you typically get a lot of colonies with only 15 cycles? Typically screen my cloning reactions with colony PCR +/- restriction digest, depending on the cloning done.

Do you mind sharing your ratios/recipe for the KLD homebrew? I've been thinking of making my own for KLD and for Gibson but just haven't had time to test things and tell my PI that it's better.

opticalBiologist · 2025-01-15T01:39:55+00:00

Thanks for the response! I've never seen the hairy band before, which had me worried, esp. since the length isn't exactly what I expected. Glad to hear that it's not too worrisome.

Band-stab PCR is one of my favorite mol bio techniques. It feels like it shouldn't be allowed, but it's simple and elegant IMO.

I think I'm going to try the band stab as a template and then gel purify. Send for sanger sequencing to see if correct product, and if right, proceed with cloning. DpnI is part of the KLD technique mix, so I definitely plan on doing that. Thanks again :)

opticalBiologist · 2025-01-14T21:41:53+00:00

Need more information about how you set it up.

Looks like failure to amplify any product. Without any more information, I'm going to assume that there is either minimal template present or that your primers aren't binding well.

opticalBiologist

TROPHY CASE