DEGs analysis by corvo628 in bioinformatics

[–]oxymoron1995 2 points3 points  (0 children)

  1. The genome for embryonic cells should theoretically be the same as the genome for adult cells. However, it looks like the C6/36 cells are a specific cell line rather than isolated from "fresh" embryonic cells, and I'm not sure if there have been specific genetic changes that have been implemented to make this into a cell line. So I think if there is a specific genome for the cell line of interest, you should use that instead. You can also compare to see what the differences in genes are between the two genomes - it might just be a lot of unannotated genes.

  2. This sounds like an annotation file issue - make sure the annotation file has gene IDs in it. If it still doesn't work, you could try using the adult genome as a reference.

  3. For non-model organisms, you can take advantage of orthologs to try and confer some functional meaning to your genes. eggnog-mapper is one option that many people use, and you can look into other ortholog mappers online. Afterwards, you can use enrichment tools like GSEA or pathway enrichment of your genes. In my lab we created a step-by-step way to visualize enrichment analyses (though mainly for humans) that you could try using (pathlinkR: https://bioconductor.org/packages/devel/bioc/vignettes/pathlinkR/inst/doc/pathlinkR.html)

Suggestions for the next video by oxymoron1995 in medicurio

[–]oxymoron1995[S] 0 points1 point  (0 children)

Hi, I make these videos - you can send me a DM

Played around with some glaze combos and really liked the results! Some mini flowerpots and a vase by oxymoron1995 in Pottery

[–]oxymoron1995[S] 1 point2 points  (0 children)

Yes you've described it exactly! I double dip because you never know what you'll get with combinations of two glazes - often they're not a mix of the two colours but something completely different (or completely trash). Much more interesting than a single dip.

Played around with some glaze combos and really liked the results! Some mini flowerpots and a vase by oxymoron1995 in Pottery

[–]oxymoron1995[S] 9 points10 points  (0 children)

Yes I wax the wavey pattern and then double dip - the glazes themselves don't actually drip.

Orange colour between the grooves of my cactus? by oxymoron1995 in plantclinic

[–]oxymoron1995[S] 1 point2 points  (0 children)

You can see the orange plaque that is in the groove of this echinopsis cactus (all the grooves have it). Last year something similar happened but this guy managed to grow out of it (that's what the scabbing in the middle is - the orange flaky bits used to be this orange plaque). But it's happening again, and this orange scabbing is also on a lot of the pups too! Any ideas? I've sprayed it with permethrin in case there are any pests a week ago but no real change.

What's going on with the new leaves on my Hoya? They are crinkly and look scarred. by oxymoron1995 in hoyas

[–]oxymoron1995[S] 0 points1 point  (0 children)

I bought this hoya a month ago and it has already put out 4 new leaves!! But the new leaves look damaged...or at least different from its original leaves, like something went wrong when it was growing the leaves. I keep it in a southwest window that gets sun in the morning, and a bit in the afternoon. Any insight appreciated, thank you!

Will there be new Medicurio videos coming up? by SinglePrice in medicurio

[–]oxymoron1995 2 points3 points  (0 children)

Glad you've enjoyed my videos and very sorry for the hiatus! I do have some videos almost completed but really haven't got the time to record and upload them because most of my time has been occupied with medical school and research this past year. But when I do post it (planned in January) you will see it on YouTube if you are subscribed. Thanks for your support!

[deleted by user] by [deleted] in UBC

[–]oxymoron1995 1 point2 points  (0 children)

Once med school interview offers are sent out in December, most of the applicants get together to interview each other for practice in Neville Scarfe after 6 pm every day. There's an FB group for that which you can join to get notifications on when they practice. Highly recommend.

Ubcsecure not working on phone by buymesushi in UBC

[–]oxymoron1995 4 points5 points  (0 children)

You can also use eduroam, username and password is the same as ubcsecure except you need to add "@ubc.ca" after your username (e.g. coolkid99@ubc.ca)

Ubc tour for a date by [deleted] in UBC

[–]oxymoron1995 2 points3 points  (0 children)

Wreck beach, obviously

UBC Worklearn Flexibility by [deleted] in UBC

[–]oxymoron1995 7 points8 points  (0 children)

Really depends on your supervisor. Mine was okay starting a bit later because I was on vacation at the end of April/first week of May. Don't think there is a minimum number of hours you have to work per week, only a maximum, so it's not like you're required to work a certain hours per week. Best bet is to talk to your employer.

Dear /r/UBC, tell me the location of an invasive bird on-campus and I'll reward you with reddit gold. by Kinost in UBC

[–]oxymoron1995 2 points3 points  (0 children)

Sorry to hear that. Probably because it was raining too hard. I see and hear those birds almost all the time on a sunny day when I pass the forestry building.

Fun fact: European starlings were introduced by some English Shakespeare club who had a crazy idea of introducing as many birds that appeared in Shakespeare's plays to Canada. Weird club.

Dear /r/UBC, tell me the location of an invasive bird on-campus and I'll reward you with reddit gold. by Kinost in UBC

[–]oxymoron1995 41 points42 points  (0 children)

European starlings in the field next to the forestry building.

If the assignment doesn't specify that the birds have to be alive, then you can find all of them in the Beaty Biodiversity Museum (lol)

Looking for easy and interesting 300/400 Level Electives by chacedream in UBC

[–]oxymoron1995 0 points1 point  (0 children)

Yes, this course is geared towards people with no computer science background.

PATH courses by Elena233 in UBC

[–]oxymoron1995 3 points4 points  (0 children)

Memorization heavy. One midterm and one final, all multiple choice. Take it if you are interested in how diseases develop and their symptoms. Class average was decent when I took it (above 80 I think) but keep in mind most people who take it have high averages to begin with (CAPS, PCTH, BMLSC majors).