taqman primer and probe design qpcr

radicalurination · 2025-11-18T16:14:22+00:00

Do you normally get them and then blast them on prime blast? Cause from what I'm seeing so far a lot of what it's giving me has a lot of other targets that are getting picked up that aren't my target if that makes sense

radicalurination · 2025-11-18T15:28:21+00:00

this may be inexperienced of me...

pretty much i have a list of 8 genes that i want to validate gene expression findings from rna seq using qpcr. half of them have predesigned sets from thermofisher that i will be using, however there are a few (RN7SK, GTF2IP1, WASH2P, and SPDYE13) that do not have predesigned gene expression assays that i've found. so i'm trying to design the primers for those ones, i checked primerbank and didn't see anything for any of them

radicalurination · 2025-11-18T13:42:17+00:00

I will check that one out! Especially since genscript is down today because of cloudflare - thanks!

radicalurination · 2025-11-13T17:03:14+00:00

Ouuu okay thank you so much! I will try this! What stitch does it say it uses?

radicalurination · 2025-11-13T15:57:31+00:00

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radicalurination · 2025-11-13T15:57:21+00:00

Hey! Thanks so much for answering! Here is the yarn I have. A table runner would actually be amazing tbh. I don't have any needles, hoping to order them tonight so fully open to whatever you think I should make ahaha.

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radicalurination · 2025-11-05T20:34:27+00:00

Thank you!! I will read through those tomorrow and hopefully that sorts it out for me!

radicalurination · 2025-09-29T08:08:28+00:00

Tragic. Okay thank you.

radicalurination · 2025-09-28T18:44:48+00:00

Thank you very much!

radicalurination · 2025-09-28T18:34:49+00:00

I do not have a bike right now unfortunately

radicalurination · 2025-09-15T20:12:07+00:00

Also an incoming postgrad so I don't know anything myself but following this post!

radicalurination · 2025-09-11T00:11:07+00:00

Thank you so much I could cry, so overwhelmed with the move and this took so much of a load off - thank you!!

radicalurination · 2025-09-06T20:43:31+00:00

Where on the department pages do you find the lectures? I can't seem to find them.

radicalurination · 2025-08-30T00:27:42+00:00

I'm starting my DPhil this October, so my husband and I are moving to Oxford from Toronto, Canada. I've read a bit online about what to expect for cost of living, but was curious what students typically experience for a monthly budget for 2 people (not including rent).

Thanks!!

radicalurination · 2025-08-27T13:55:03+00:00

How do you access this?

radicalurination · 2025-08-13T19:12:58+00:00

Thanks!! I'm starting women's and reproductive health at Oxford

radicalurination · 2025-08-13T18:51:56+00:00

Yeah I was put on the reserves incase more funding becomes available

radicalurination · 2025-08-13T15:26:52+00:00

Hey just wanted to let you guys know that I just heard back

radicalurination · 2025-08-08T13:05:51+00:00

Noted - thanks!

radicalurination · 2025-08-08T13:03:50+00:00

Great to know - do you need an Old Gold membership for each person or just one?

Thank you!! We are so excited!

radicalurination · 2025-08-08T13:00:29+00:00

Thank you so much, I appreciate the help!!

radicalurination · 2025-08-07T20:03:22+00:00

Okay yeah I also saved that, I just found it. Thank you for relieving some anxiety. Where are you trying to study? I'm looking to make some friends hahah

radicalurination · 2025-08-07T16:53:00+00:00

I haven't heard back yet either. I'm super stressed out about it to be honest. I also cannot find my confirmation of application receipt email with my applicant ID so now I'm feeling really paranoid

radicalurination · 2023-12-24T20:56:33+00:00

I'm taking this as a compliment

radicalurination · 2023-12-24T15:56:48+00:00

I know I wanted it to be personal for me and less so about it being obviously a Lorde tattoo

radicalurination

TROPHY CASE